Phospholipid-polyaniline nanoparticles for photoacoustic imaging and/or photothermal therapy and preparation method

A photothermal therapy and photoacoustic imaging technology, applied in the field of medicine, can solve the problems of complex preparation process, low biocompatibility, low yield, etc., and achieve the effects of simple preparation method, strong near-infrared absorption, and low energy consumption.

Inactive Publication Date: 2016-05-04
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported polyaniline nanoparticles used for photothermal therapy use surfactants such as poloxamer, polyethylene glycol, etc. as stabilizers, which have low biocompatibility, and the preparation process is complicated and the yield is low.
In addition, there are few reports about polyaniline nanomaterials as contrast agents for photoacoustic imaging.

Method used

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  • Phospholipid-polyaniline nanoparticles for photoacoustic imaging and/or photothermal therapy and preparation method
  • Phospholipid-polyaniline nanoparticles for photoacoustic imaging and/or photothermal therapy and preparation method
  • Phospholipid-polyaniline nanoparticles for photoacoustic imaging and/or photothermal therapy and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of intrinsic state polyaniline: (adopt oxidation polymerization method, this method is reported in the literature)

[0038] Add 0.2mol aniline monomer and 0.3mol concentrated hydrochloric acid to 300mL water to obtain solution a; add 0.1mol ammonium persulfate and 0.2mol concentrated hydrochloric acid to 200ml water to obtain solution b; under stirring, add solution b dropwise to the solution In a, stir at 4°C for 6 hours to obtain a polymer salt solution, which is filtered; disperse all the obtained green precipitates into 500 ml of 1M aqueous sodium hydroxide solution for dedoping reaction, filter, and remove all the obtained purple The precipitate was dispersed into 500 ml of acetone, filtered, and dried under vacuum for 20 hours to obtain purple intrinsic polyaniline powder, which is called EBPANI for short. Gel chromatographic column detection molecular weight 5000.

Embodiment 2

[0040] A method for preparing phospholipid-polyaniline nanoparticles for photoacoustic imaging and / or photothermal therapy, comprising the steps of:

[0041] (1) the intrinsic state polyaniline (the intrinsic state polyaniline is called for short EBPANI) that molecular weight is 5000 is prepared by oxidative polymerization method (see embodiment 1);

[0042] (2) Synthesis of polyaniline nanoparticles wrapped in phospholipids: 5 mg of the intrinsic polyaniline obtained in step (1) and 50 mg of sodium dodecylbenzenesulfonate were dissolved in 8 mL of chloroform; stirred at room temperature for 18 hours to obtain a green Conductive polyaniline solution (conductive polyaniline referred to as ESPANI), 8mL conductive polyaniline solution, 20mL chloroform, 35.2mgDPPC, 5mgDSPE-PEG2000 and 1.4mgFA-DSPE-PEG2000 were added to the container, ultrasonically dispersed for 4 minutes; Evaporate under reduced pressure at 50°C and 120rpm for 25 minutes to remove chloroform, and let stand in vac...

Embodiment 3

[0044] A method for preparing phospholipid-polyaniline nanoparticles for photoacoustic imaging and / or photothermal therapy, comprising the steps of:

[0045] (1) the intrinsic state polyaniline that molecular weight is 5000 is prepared by oxidative polymerization method (see embodiment 1);

[0046](2) Synthesis of polyaniline nanoparticles wrapped in phospholipids: 5 mg of the intrinsic polyaniline obtained in step (1) and 35 mg of sodium dodecylbenzenesulfonate were dissolved in 5 mL of chloroform; stirred at room temperature for 12 hours to obtain a green Conductive polyaniline solution (conductive polyaniline referred to as ESPANI), 5mL conductive polyaniline solution, 20mL chloroform, 35.2mgDPPC, 4mgDSPE-PEG2000 and 1.2mgFA-DSPE-PEG2000 were added to the container, ultrasonically dispersed for 2 minutes; Evaporate under reduced pressure at 40°C and 100rpm for 20 minutes to remove chloroform, and stand in vacuum for 6 hours to remove residual chloroform, add 5mL of water, u...

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Abstract

The invention discloses phospholipid-polyaniline nanoparticles for photoacoustic imaging and / or photothermal therapy and a preparation method. The preparation method comprises the steps that 1, eigenstate polyaniline is prepared; 2, phospholipid-coated polyaniline nanoparticles are synthesized, wherein eigenstate polyaniline and sodium dodecyl benzene sulfonate are dissolved into chloroform, stirring is performed to obtain a green conductive polyaniline solution, the conductive polyaniline solution, chloroform, DPPC, DSPE-PEG2000 and FA-DSPE-PEG2000 are added into a container, ultrasonic dispersion is performed, chloroform is removed through evaporation, water is added, ultrasonic dispersion and centrifugation are performed, supernate is a clear and bright green solution, and then freeze drying is performed. The preparation method has the advantages of being simple, high in reaction controllability, low in energy dissipation and capable of easily achieving scalization and easily obtaining the raw materials; the prepared nanoparticles show the good water solubility and biocompatibility, the high near-infrared absorption performance and the good light and thermal stability and can be simultaneously used for photothermal therapy, photoacoustic imaging and infrared thermal imaging.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a polyaniline nanoparticle used for photoacoustic imaging and photothermal therapy and a preparation method. Background technique [0002] Photothermal therapy is a minimally invasive tumor treatment technology developed in recent years. It mainly kills tumor cells by directly irradiating light energy to the tumor site to increase its local temperature, which greatly reduces systemic toxicity. Therefore, photothermal therapy It is regarded as one of the techniques for treating tumors with great potential to replace surgery. To improve the efficiency and tumor selectivity of laser-induced photothermal therapy, photothermal therapeutic agents with light-absorbing properties are usually introduced into tumor sites. Since water and protein in biological tissues absorb near-infrared light relatively weakly, so near-infrared light has the best penetrability to tissue, an ideal photothermal thera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/22A61K47/24
CPCA61K41/0052A61K47/24A61K49/221A61K49/225
Inventor 王金平过芳于梦谭丰苹李楠
Owner TIANJIN UNIV
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