Plant antibacterial method implemented by using methylotrophic bacillus nkg‑1

A methylotrophic, bacillus technology, applied in the fields of botanical equipment and methods, plant protection, plant growth regulators, etc., can solve problems such as endangering human health, destroying ecological balance, pesticide environmental pollution, etc., and achieving a pollution-free environment. , No effect of pesticide residues

Active Publication Date: 2017-03-22
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and large-scale application of chemical pesticides leads to pesticide residues and environmental pollution, which eventually endangers human health and destroys the ecological balance. At the same time, it also makes pathogens highly resistant to various chemical fungicides

Method used

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  • Plant antibacterial method implemented by using methylotrophic bacillus nkg‑1
  • Plant antibacterial method implemented by using methylotrophic bacillus nkg‑1
  • Plant antibacterial method implemented by using methylotrophic bacillus nkg‑1

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0093] Preparation of Methylotrophic Bacillus Fermentation Culture Filtrate Methylotrophic Bacillus was inoculated in LB medium at a ratio of 1:100, shaken at 37°C and 220r / min for 24h. The fermentation broth was collected, centrifuged (10000r / min, 4°C), and the supernatant was sterilized by filtration with a 0.22 μm filter membrane to obtain a sterile fermentation broth.

[0094] Using the mycelial growth rate method to detect the effects of antagonistic bacillus on tomato gray mold, tomato leaf mold, rice blast, pepper anthracnose, apple ring spot, apple rot, cucumber anthracnose, wheat sheath blight, wheat gibberella Blight, asparagus stem rot, corn small spot, cotton wilt, grape black rot, tobacco red spot, peach rhizopus soft rot, and cotton seedling blight.

[0095] Under sterile conditions, take 10 mL of the prepared Bacillus sterile fermentation broth and 90 mL of the melted PDA medium and mix them evenly, and make a plate culture medium containing fermentation broth i...

Embodiment 1

[0104] Embodiment 1: Grape black rot antibacterial method

[0105] Picking the marginal hyphae of confrontation culture, microscopic observation found that the antibacterial effect of NKG-1 on grape black rot mainly manifested as the effect on mycelia and spores. Various metabolites of NKG-1 (including antibacterial proteins, bacteriocins, antibiotics, various cell wall degrading enzymes, etc.) destroy the mycelial wall structure of pathogenic bacteria, causing mycelial expansion, distortion, distortion, and mycelial wall digestion, etc. The middle of the spore constricts, the cell wall dissolves, and the protoplasm leaks out.

[0106] The antibacterial effect of methylotrophic bacillus NKG-1 on grape black rot fungus is as follows: Figure 10 shown.

[0107] Table 2. Mycelia inhibitory effect of methylotrophic Bacillus NKG-1 on grape black rot

[0108]

[0109] (1) Determination results of the effect of methylotrophic bacillus NKG-1 strain fermentation liquid on control...

Embodiment 2

[0126] Embodiment 2: Bacteriostasis method for asparagus stem rot

[0127] The antibacterial effect of methylotrophic bacillus NKG-1 on asparagus stem rot fungus is as follows: Figure 17 shown.

[0128] Table 5. Mycelia inhibitory effect of methylotrophic Bacillus NKG-1 on stem rot of asparagus

[0129]

[0130] Determination of the Control Effect of Methylotrophic Bacillus NKG-1 Strain on Stem Rot of Asparagus in Greenhouse

[0131] Three treatments were set up in the field test: A. biocontrol agent NKG-1500 times liquid; B. 25% metalaxyl wettable powder 600 times liquid; C. blank control. 14m per cell 2 , 3 rows of isolation areas were set up between the plots, and each treatment was arranged in a completely randomized block group, repeated 3 times, and a total of 9 plots were used in the whole experiment. Before the test, divide the plots according to the test plan, open the isolation ditch, and use 15000mL of 1×10 per hectare. 7 cfu / mL biocontrol agent NKG-1. The...

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Abstract

The invention discloses a plant bacteriostasis method adopting the bacillus methylotrophicus strain NKG-1. Pesticide is prepared from bacillus methylotrophicus strain NKG-1 bacteria liquid, and the prepared pesticide methylotrophicus agentia is applied in the growing period of plants to inhibit germs on the plants. By the adoption of the plant bacteriostasis method adopting the strain, various pathogenic fungi and bacteria can be inhibited, a good control effect is realized, and therefore application prospects are broad.

Description

technical field [0001] The present invention relates to a plant antibacterial method implemented by methylotrophic bacillus NKG-1, in particular to a plant antibacterial method implemented by methylotrophic bacillus (Bacillus methylotrophicus strain NKG-1), which can effectively Inhibit the infection of pathogenic bacteria. Background technique [0002] Plant diseases can cause serious economic losses to agricultural production. At present, pesticides are usually used to control plant diseases and insect pests at various growth stages. Among them, chemical pesticides are mainly applied, which have the characteristics of convenient fertilization and quick results. However, the long-term and large-scale application of chemical pesticides leads to pesticide residues and environmental pollution, which eventually endangers human health and destroys the ecological balance. The use of living microorganisms for biological control has been widely valued by countries all over the wor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P1/00A01P3/00A01G13/00
CPCA01G13/00A01N63/00
Inventor 葛蓓孛张克诚刘炳花刘艳赵文珺辛辛诺
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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