A kind of iota collagen lyotropic liquid crystal three-dimensional gel preparation method

Abstract

The invention discloses a preparation method of type I collagen lyotropic liquid crystal three dimensional gel. The method comprises the following steps: directionally shearing and concentrating an acidic telopeptide-free type I collagen dilute solution in a tangential flow system at a low temperature until the concentration is 1-3 times the concentration of the original dilute solution and the viscosity does not change, wherein the pH value of the acidic type I collagen dilute solution is 2-4; placing the concentrated acidic collagen fluid in a polyethylene glycol solution, and carrying out dialysis dehydration until the concentration increases 10-40 times to obtain a high-viscosity liquid crystal collagen fluid, wherein the mass / volume concentration of the polyethylene glycol solution is 20-50%; and placing the high-viscosity liquid crystal collagen fluid in an alkaline gas generator, carrying out sealed disposing for 10min-24h, hatching to adjust the environment temperature to 4-50DEG C, and regulating microfiber self-assembling and fiber branch cross-linking through acidic and alkaline environment to obtain the type I collagen lyotropic liquid crystal three dimensional gel. The preparation method has the advantages of non-toxicity, no pollution and short time; and the gel has uniform structure and high ordered degree.

Description

technical field [0001] The invention belongs to the technical field of preparation of bionic biology, biomedical materials, tissue engineering scaffolds and the third type of implanted medical device materials, and in particular relates to a preparation method of type I collagen lyotropic liquid crystal three-dimensional gel. Background technique [0002] Type Ι collagen is the main structural protein of extracellular matrix in connective tissue (such as bone, cornea, tendon, ligament and skin, etc.). This structural protein is arranged in a highly ordered manner in connective tissue, and the arrangement of collagen fibers in different connective tissues has a liquid crystal geometric structure. The liquid crystal state of connective tissue not only interacts with cell adhesion, extension, growth, proliferation, and directional differentiation, but also strongly controls the biomechanics and other biophysical properties of connective tissue. Collagen fibers are the main com...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing type I collagen lyotropic liquid crystal three-dimensional gel, aiming to solve the problem that the liquid crystal collagen prepared by the current method for preparing liquid crystal collagen has different concentration gradients, takes a long time, and the prepared Collagen liquid crystal structure is not uniform, it is difficult to obtain three-dimensional gel state liquid crystals, and there are few applications in the fields of biomimetic biology, biomedical materials, tissue engineering scaffolds, and third-class medical devices.

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