Preparation method for rare ginsenoside C-K and F1 and four kinds of isomer ginsengenin

A technology for rare ginseng saponins and isomers, which is applied to the preparation of F1 and four isomers of ginsenosides, and the field of rare ginseng saponins C-K, can solve the problems of many by-products, low conversion rate, great difficulty, etc. High yield and purity, simple operation and low cost

Active Publication Date: 2016-06-08
金凤燮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0026] Therefore, the enzymatic conversion method disclosed in the prior art hydrolyzes panaxadiol saponins Rb1, Rb2, Rb3, Rc, Rd to prepare C-K saponins or the enzyme conversion method hydrolyzes panaxadiol saponins Re, R1, Rg1 to prepare F1 saponins, both exist There are many by-products, the conversion rate of rare

Method used

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  • Preparation method for rare ginsenoside C-K and F1 and four kinds of isomer ginsengenin
  • Preparation method for rare ginsenoside C-K and F1 and four kinds of isomer ginsengenin
  • Preparation method for rare ginsenoside C-K and F1 and four kinds of isomer ginsengenin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] a. Using ginseng as a raw material, extracting a ginseng diol saponin mixture and a panaxatriol saponin mixture:

[0050] Ginseng (with fibrous root) 1 kg, cut into slices (2-4mm thick), soak in 8 liters of methanol, stir and extract at 35-40°C for 6-12 hours, separate the extract, repeat three times; filter, combine the extract, reduce Concentrate under pressure (recover methanol) to a specific gravity of Baumé 20, add 500 ml of petroleum ether, stir and degrease for 1 hour, separate the grease layer, and repeat 2 times. Dilute the degreased ginseng extract with 3 to 5 times the volume of water, repeatedly absorb saponin in a 1.5 liter volume macroporous resin (AB-8 resin or HP-20 resin) column, and use 7.5 to 10 liters of deionized The water elution column removes sugar and other impurities; the macroporous resin column that absorbs saponin is eluted with ethanol-water gradient: the ethanol concentration gradient is 30% to 84%, the total volume of eluent is 11 liters,...

Embodiment 2

[0063] a. Taking ginseng as a raw material, extracting a mixture of panaxatriol saponins is the same as in Example 1;

[0064] b. Use ginseng triol saponin mixture as raw material, prepare substrate solution with organic solvent and buffer solution, and then react substrate solution with crude enzyme solution obtained from Aspergillus microbial fermentation:

[0065] The crude enzyme solution is prepared according to the following conventional methods:

[0066]Aspergillus oryzae sp.39g (isolated from Daqu; literature, Wang Dongming, Yu Hongshan, etc. Process Biochemistry 2012, 47, 133-138. Dalian University of Technology Culture Collection, preservation number: Aspergillusoryzaesp.39g) was inoculated in a sterilized 1 kg of wheat bran with a water content of 50% (containing 50 g of ginseng powder), cultured at 28-32°C for 4-7 days (stirred 4-6 times a day), and then mixed with 5 liters of 0.02 mole, pH 5. 0 acetic acid buffer to leach the enzyme; the enzyme liquid is centrifu...

Embodiment 3

[0074] Preparation of ginseng self-saponin enzyme complex: take 1 kg of ginseng extract saponin slag described in Example 1, add 6 liters of deionized water, stir at 55-65°C for 2-3 hours, centrifuge to collect supernatant, concentrate in high vacuum (product temperature below 65°C) to Baume degree 25 (about 500 ml), it is the saponinase complex of ginseng itself.

[0075] React the obtained ginseng self-saponinase complex with C-K obtained in Example 1, add formic acid, acetic acid or citric acid with a reaction volume of 0.1 to 50% in the reaction system to reduce the pH reaction, and prepare four isomers of ginseng Diol saponin.

[0076] The details are as follows: Take 20 grams of the product C-K saponins of Example 1, 50 grams of ginseng autosaponinase complex, 350 milliliters of deionized water, and 25 grams of citric acid, mix them, and react at 70-75° C. for 2 hours. Use TLC method to detect, after the reaction is complete, add 400 ml of water-saturated n-butanol to e...

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Abstract

The invention discloses a preparation method for rare ginsenoside C-K and F1 and four kinds of isomer panoxadiol saponin and panaxatriol sapogenin. Ginseng is used as a raw material, panoxadiol saponin (mixture), panaxatriol sapogenin (mixture) and a ginseng saponin enzyme compound are extracted. Panoxadiol saponin and an organic solvent are prepared into a substrate solution, panaxatriol sapogenin and a buffer solution are prepared into a substrate solution, the two substrate solutions are fermented with aspergillus microorganisms to obtain crude enzyme liquid for a reaction, there is almost no other byproduct saponin, and rare ginsenoside C-K or rare ginsenoside F1is obtained; after the reaction, the enzyme can be recovered and used repeatedly. The obtained C-K or F1 saponin reacts with the ginseng saponin enzyme compound (a proper amount of organic acid is added), and then four kinds of isomer panoxadiol saponin and panaxatriol sapogenin are obtained. Operation is easy, yield is high, cost is low, and the method is suitable for mass production; the obtained products can be used for medicine development, ginseng products, health care products and cosmetics.

Description

technical field [0001] The present invention relates to a preparation method of ginseng rare saponins and four isomer ginsenosides, especially a kind of ginseng rare saponins C-K, F1 and four ginsenosides which are simple in operation, low in cost, high in yield and purity, and suitable for mass production. A preparation method of isomer ginsenogenin. Background technique [0002] The ginseng with the highest yield in my country is mainly ginseng (PanaxginsengC.A.Mayer), American ginseng (P.quinguefolusL.) and Panax notoginseng (P.notoginsengburk). The main saponins of ginseng root are Rb1, Rb2, Rb3, Rc, Rd, Re, Rg1; the main saponins of American ginseng are mainly Rb1 and Re, and also contain Rb2, Rc, Rd, Rg1 saponins; the main saponins of Panax notoginseng are Rb1 Mainly and Rg1, also contains Rd, Re, R1 saponins; other saponins content is low. [0003] The main protopanaxadiol (PPD) saponin structure of ginseng is as follows: [0004] [0005] [0006] Note: Glc,...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12P33/00
CPCC12P33/00C12P33/20
Inventor 鱼红闪刘春莹金凤燮
Owner 金凤燮
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