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A preparation method of temperature-sensitive self-polishing resin covalently immobilized antifouling enzyme

A technology of self-polishing resin and covalent immobilization, which is applied in antifouling/underwater coatings, biocide-containing paints, coatings, etc., and can solve the problems of single immobilization method of antifouling enzymes, poor environmental adaptability, and unsatisfactory performance , to achieve the effect of good antifouling performance, low production cost and improved stability

Active Publication Date: 2017-10-27
725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent with publication number CN102137900A discloses an antifouling composition in which bioactive agents and enzymes are embedded in airgel; the patent with publication number CN102083919B discloses an antifouling composition comprising a first enzyme and an encapsulated second enzyme. The pollution composition uses β-galactosidase, peptidase, glucoamylase and their mixture as the first enzyme, and selects the autooxidase encapsulated in silicate as the second enzyme; the patent publication with the publication number CN102307956B An enzyme-based self-polishing coating composition comprising a binder phase in the form of a binder system and a pigment phase comprising a polysaccharide and an enzyme that can assist in the hydrolysis of the polysaccharide, the enzyme being glucoamylase, And polysaccharide can be starch; Publication number is that the patent of CN103819983A discloses a kind of antifouling paint that is made up of antifouling agent such as polymer resin, capsaicin, osthole, algae alcohol and protease; Publication number is CN101284956B The patent discloses a preparation method for protease-producing bacteria obtained from separation and purification of barnacles and mussels and protease-based antifouling coatings; in addition, the Danish company Biolocus has developed a variety of enzyme preparations for marine antifouling and cooperated with JOTUN The company has cooperated to produce enzyme coatings, which have been popularized and applied on speedboats; GENECORE and HEMPEL have jointly developed antifouling coatings based on the joint production of hydrogen peroxide by hexose enzyme and catalase, but these are based on Enzyme antifouling coatings have the defects of single antifouling enzyme immobilization method, poor environmental adaptability and performance that cannot meet the marine fouling environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The preparation method of the temperature-sensitive self-polishing resin for covalently immobilizing antifouling enzymes involved in this example includes two steps: preparation of temperature-sensitive self-polishing resins and covalently immobilizing antifouling enzymes:

[0013] (1) Preparation of temperature-sensitive self-polishing resin: Add 2L of ultrapure water into a three-necked bottle with a capacity of 5L, place the three-necked bottle on a stirrer and stir at a speed of 500rpm, and gradually add 20g to the three-necked bottle while stirring Methyl methacrylate and 30g of trimethylsilyl methacrylate, under nitrogen protection, after the stirring, add 500 μl of n-dodecanethiol into the three-necked flask, heat the three-necked flask to 80°C, and add 50ml of 0.03g / ml ammonium persulfate aqueous solution, put the three-necked bottle on the stirrer and stir at a speed of 400rpm for 3.5h. After the stirring, add 5g of glycidyl methacrylate dropwise to the three-ne...

Embodiment 2

[0018] The preparation method of the temperature-sensitive self-polishing resin for covalently immobilizing antifouling enzymes involved in this example includes two steps: preparation of temperature-sensitive self-polishing resins and covalently immobilizing antifouling enzymes:

[0019] (1) Preparation of temperature-sensitive self-polishing resin: Add 2L of ultrapure water into a three-necked bottle with a capacity of 5L, place the three-necked bottle on a stirrer and stir at a speed of 500rpm, and gradually add 20g to the three-necked bottle while stirring Methyl methacrylate and 30g of trimethylsilyl methacrylate, under nitrogen protection, after the stirring, add 500 μl of n-dodecanethiol into the three-necked flask, heat the three-necked flask to 80°C, and then add 50ml of 0.03g / ml ammonium persulfate aqueous solution, put the three-necked bottle on the stirrer and stir at a speed of 400rpm for 3.5h. After the stirring is completed, add 5g of glycidyl methacrylate dropwi...

Embodiment 3

[0022] The preparation method of the temperature-sensitive self-polishing resin for covalently immobilizing antifouling enzymes involved in this example includes two steps: preparation of temperature-sensitive self-polishing resins and covalently immobilizing antifouling enzymes:

[0023] (1) Preparation of temperature-sensitive self-polishing resin: Add 2L of ultrapure water into a three-necked bottle with a capacity of 5L, place the three-necked bottle on a stirrer and stir at a speed of 500rpm, and gradually add 45g to the three-necked bottle while stirring Methyl methacrylate and 5g of trimethylsilyl methacrylate, under nitrogen protection, after the stirring, add 800 μl of n-dodecanethiol into the three-necked flask, heat the three-necked flask to 80°C, and then add 50ml of 0.03g / ml ammonium persulfate aqueous solution, put the three-necked bottle on the stirrer and stir at a speed of 400rpm for 5.5h. After the stirring is completed, add 30g of acetoacetate methacrylate dr...

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PUM

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Abstract

The invention belongs to the technical field of marine biological fouling protection, and relates to a method for preparing a temperature-sensitive self-polishing resin covalently immobilized with an antifouling enzyme, comprising two steps of preparing the temperature-sensitive self-polishing resin and covalently immobilizing the antifouling enzyme. The molecular segments of the resin synthesized by the oil-in-water emulsion polymerization method contain glycidyl groups, acetoacetate groups, acrylate silicon ester components and long-chain alkyl acrylates, and the glycidyl groups and acetoacetate groups in the buffer solution It forms a covalent bond with the amino group of the antifouling enzyme to improve the stability of the antifouling enzyme. The silicon acrylate component has polishing properties and is continuously renewed on the surface of the material. The long-chain alkyl acrylate is temperature sensitive and changes with seawater temperature Regulating the release of antifouling enzymes, the synthesis process is based on the water phase and does not contain heavy metal pollutants; the process is simple, the production cost is low, the environment is friendly, and the antifouling performance is good, which can be widely used on the surface of marine ships and structures.

Description

Technical field: [0001] The invention belongs to the technical field of protection against marine biological fouling, and relates to a method for preparing a temperature-sensitive self-polishing resin covalently immobilized with an antifouling enzyme. The synthesis process is based on an oil-in-water emulsion polymerization method, and the prepared resin contains acrylic acid long-chain alkyl groups. esters, glycidyl, acetoacetate acrylates and hydrolyzable silicon acrylates. Background technique: [0002] Marine fouling causes serious harm to ships and marine artificial facilities, affects their navigation speed, fuel economy and safety, and increases maintenance and repair costs. Therefore, effective measures must be taken to prevent marine organisms from fouling and attaching to them. Traditional organotin antifouling coatings have been banned due to serious harm to the marine ecological environment; currently widely used antifouling coatings containing cuprous oxide and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D5/16C08F275/00C08F265/06C08F220/18C08F220/32C08F220/28C08F2/24
CPCC08F265/06C08F275/00C09D5/1637C08F220/283C08F220/1804
Inventor 王利蔺存国
Owner 725TH RES INST OF CHINA SHIPBUILDING INDAL CORP