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Method for preparing hulless oat blood sugar reducing polypeptide with hulless oat albumen powder

A technology of hypoglycemic polypeptide and protein powder, which is applied in hydrolyzed protein components, peptide/protein components, protein food processing, etc., can solve problems such as poor high-density lipoprotein content, and achieve polysaccharide metabolism, hypoglycemic health care, safety, toxicity, and side effects. The effect of simple technical process

Active Publication Date: 2016-06-29
内蒙古佟明阡禾食品有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The alloxan diabetic mouse model test revealed that, like Baisupine, both oatmeal polysaccharide and protein can maintain the serum triglyceride content at a relatively low level. Improvement of serum high-density lipoprotein levels is worse than buckwheat protein and glucosamine

Method used

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  • Method for preparing hulless oat blood sugar reducing polypeptide with hulless oat albumen powder
  • Method for preparing hulless oat blood sugar reducing polypeptide with hulless oat albumen powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 Preparation of naked oat hypoglycemic polypeptide

[0023] Weigh naked oats defatted protein powder and pure water, mix the ingredients at a weight ratio of 1:500, stir well, adjust the pH to 1.5 with 1M hydrochloric acid, and then add the enzyme to substrate (naked oats defatted protein) mass ratio of 1:50 into the stomach Protease, incubate at 37°C for 1 hour, carry out enzymolysis reaction; then adjust the obtained enzymolysis solution to pH 8.0 with 1M NaOH; Trypsin, α-chymotrypsin, and elastase were subjected to secondary enzymatic hydrolysis for 1 hour at 37°C; the polypeptide solution after the secondary enzymatic hydrolysis was placed in a water bath at 95°C for 15 minutes to inactivate the oatmeal hypoglycemic polypeptide solution.

[0024] Take naked oat defatted protein, primary enzymatic hydrolysis solution, and secondary enzymatic hydrolysis solution samples respectively, and carry out Tricine-SDS detection. The results are as follows: figure ...

Embodiment 2

[0025] Embodiment 2 Determination of naked oat polypeptide inhibiting α-glucosidase activity

[0026] α-glucosidase assay system: In a 0.5mL reaction system, the concentration of the substrate pNP-α-Glu (4Nitrophenyl-α-D-g1ucopyranosodide) is 3×10 -4 mol / L, the concentration of reduced glutathione is 8.125×10 -5 mol / L, the enzyme concentration is 0.5U / mL; after reacting accurately at 37°C for 10 minutes, add stop solution (0.1mol / LNa 2 CO3 solution) 2.5mL, measure A 410nm .

[0027] Definition of α-glucosidase activity unit: at pH 6.8, 37°C, release 1 μmol of PNP per minute as an enzyme activity unit.

[0028] The naked oat polypeptide prepared in Example 1, acarbose (purchased from Sichuan Luye Baoguang Co., Ltd.), naked oat defatted protein (the protein and polysaccharide components in naked oats have effects on blood sugar and blood lipids in alloxan-induced diabetic mice Comparison, Food Science and Technology, 2013, 38 (1): 66-70), buckwheat polysaccharide (purchased ...

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Abstract

The invention provides a method for preparing hulless oat blood sugar reducing polypeptide with hulless oat albumen powder. The method comprises the steps that degreased hulless oat albumen powder is added into pure water at the material to liquid ratio of 1:(100-500), the pH value is regulated to be 1.0-3.0 with 1M hydrochloric acid, pepsin is added according to E / S of 1:(50-100), enzymolysis is carried out for 1-4 h at 37 DEG C, and primary enzymatic hydrolysate is obtained; the pH value of the primary enzymatic hydrolysate is regulated with 1M NaoH to be 7.0-8.0, then trypsin, alpha-chymotrypsin and elastase are added according to E / S of 1:(50-100), enzymolysis is carried out for 1-4 h at 37 DEG C, and secondary enzymatic hydrolysate is obtained; the secondary enzymatic hydrolysate is heated to 95 DEG C for enzyme denaturalixation for 15 min, spraying drying is carried out, and polypeptide containing alpha glucosidase inhibiting activity is obtained. The process is simple, the production cost is low, and the product is safe and free of toxic or side effects. The hulless oat blood sugar reducing polypeptide prepared through the method can be applied in preparing medicine or health-care products for preventing and treating diabetes.

Description

technical field [0001] The invention relates to a method for deep processing naked oat protein powder, in particular to a method for preparing naked oat hypoglycemic polypeptide with naked oat protein powder. Background technique [0002] Naked oats, the scientific name is naked oats, is a unique food crop in my country, and is also a characteristic small miscellaneous grain in Shanxi. It is one of the world-recognized food crops with high nutritional value. Its protein content is 75% higher than that of corn and 66% of wheat. It is 105.3% and 132.8% higher than that of indica rice and japonica rice respectively. The water-soluble dietary fiber of oatmeal is 3.5 times that of wheat, 1.4 times that of corn, and 7.0 times that of japonica rice. Naked oats have a variety of health effects, such as lowering blood fat and cholesterol, anti-atherosclerosis, controlling and lowering blood sugar, lowering blood pressure, promoting children's growth and delaying the aging process of t...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P3/10A23J3/14A23J3/34A23L33/18A23L33/185
CPCA23J3/14A23J3/341A23V2002/00A61K38/011C12P21/06A23V2200/328A23V2250/55
Inventor 石亚伟焦慧娟
Owner 内蒙古佟明阡禾食品有限责任公司
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