Alkaline beta-mannase, encoding genes thereof and application of encoding genes
A mannanase, alkaline technology, applied in the field of genetic engineering, can solve the problems of low enzyme activity, low expression, poor stability, etc., and achieve the effect of high activity, high activity and strong stability
Active Publication Date: 2016-07-13
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Abstract
The invention discloses alkaline beta-mannase as shown in (a) or (b).The alkaline beta-mannase as shown in (a) is formed by the amino acid sequence as shown in SEQ ID No.1 or SEQ ID No.3. (b) is protein derived from (a) with unchanged enzyme activity, and is obtained by carrying out substituting, deleting or adding one or more amino acids on the amino sequence shown in SEQ ID No.1 or SEQ ID No.3 , or b is protein with amino sequence of SEQ ID No.1 or SEQ ID No.3 with tags on the amino terminal and/or carboxyl terminal. The invention further discloses genes capable of encoding the alkaline beta-mannase, a recombinant vector containing the genes, a recombinant strain containing the recombination vector and application of the genes, the recombinant vector and the recombinant strain.The invention also discloses a method for preparing the alkaline beta-mannase and a composition for degrading the mannase.The alkaline beta-mannase is high in heat resistance, activity and stability.
Application Domain
FungiBacteria +5
Technology Topic
Amino terminalEnzyme +7
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PUM
Property | Measurement | Unit |
Specific vitality | 330.0 ~ 1800.0 | U/mg |
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