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Method for measuring amino acid in mainstream cigarette smoke

A technology for cigarette mainstream smoke and determination methods, applied in the field of physical and chemical testing, to achieve good repeatability and recovery, high accuracy, and overcome the effects of matrix effects

Inactive Publication Date: 2016-08-31
CHINA TOBACCO GUANGXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the detection methods of amino acids in cigarette smoke.

Method used

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  • Method for measuring amino acid in mainstream cigarette smoke
  • Method for measuring amino acid in mainstream cigarette smoke
  • Method for measuring amino acid in mainstream cigarette smoke

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Experimental program
Comparison scheme
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Embodiment 1

[0045] Instruments, reagents, and materials: AB SCIEX 5500LC-MS / MS from the United States; Agilent 1290HPLC from the United States; Agilent ZORBAX Eclipse AAA column (150mm×4.6mm, particle size 5μm), 0.22μm microporous filter membrane, ultrasonic cleaner; ultrapure water (18MΩ), acetonitrile (chromatographically pure, Bailingwei Chemical Reagent Company), 17 kinds of amino acid mixed standards.

[0046] Preparation of the extract: use concentrated hydrochloric acid to prepare a 0.05mol / L dilute HCl solution as the extract;

[0047] Preparation of internal standard solution: weigh a certain amount of leucine-d 3 , use the extract to prepare an internal standard solution with a concentration of 0.1 μmol / mL;

[0048] Preparation of mixed standard solutions: using the extract as a solvent, mix 17 kinds of amino acids into standard solutions of different concentrations, proline, alanine, isoleucine, leucine, arginine, histidine , methionine, phenylalanine, serine, threonine, tyro...

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Abstract

The invention discloses a method for measuring amino acid in mainstream cigarette smoke. The method includes the steps that amino acid in the mainstream cigarette smoke is captured by means of a Cambridge filter; after a cigarette is smoked, the Cambridge filter collecting a mainstream cigarette smoke sample is taken out, after an internal standard is added, ultrasonic extraction is conducted by means of a diluted hydrochloric acid solution, a filtering film enters an efficient liquid chromatogram-tandem mass spectrometer for measuring, and quantitation is conducted through the internal standard method. The method is characterized in that quantitation avoids influences of the matrix effect in the analysis process by means of the isotopic dilution internal standard method; direct sample injection is adopted so that the content of 17 amino acids in the mainstream cigarette smoke can be quickly and accurately detected, repeatability and the recovery rate are good, and the method is suitable for analysis of a large quantity of samples in industry.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical testing, and in particular relates to a method for measuring amino acid content in cigarette mainstream smoke. Background technique [0002] Amino acid is the most basic substance that constitutes the protein of the organism, and is one of the indispensable components in the organism. It not only has a variety of physiological functions, but also is widely used in food additives as a taste agent and a source of nutrition. Amino acids are an important class of compounds in tobacco. During tobacco preparation, alcoholization, fermentation processing, shred drying and burning, there are enzymatic and non-enzymatic brown reactions between free amino acids and reducing sugars (or carbonyl compounds), forming A variety of pyran, pyrazine, pyrrole, pyridine and other heterocyclic compounds with roasted or popcorn aroma characteristics, some amino acids such as phenylalanine amino acid can ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/027G01N2030/8818
Inventor 李小兰范忠刘鸿陈峰黄善松周芸许蔼飞吴晶晶孟冬玲王维刚蒋光辉
Owner CHINA TOBACCO GUANGXI IND