Method for preparing bacillus subtilis traditional Chinese medicine feed additive

A technology for Bacillus subtilis and feed additives, which is applied in the field of preparation of bacteria powder, can solve the problems of uneven quality of Bacillus subtilis, unscientific composition of ingredients, backward equipment and technology, etc., and achieves accelerated animal growth rate and broad market application prospects. , the effect of promoting rapid growth

Inactive Publication Date: 2016-09-21
河南省岳氏精忠科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are many Bacillus subtilis production enterprises in the market, but most of them are small-scale enterprises, workshop-style production, backward equipment and technolog

Method used

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  • Method for preparing bacillus subtilis traditional Chinese medicine feed additive

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Effect test

Embodiment 1

[0018] A preparation method of bacillus subtilis traditional Chinese medicine feed additive, comprising the following steps:

[0019] Step 1, inoculate Bacillus subtilis for feed on the slant seed medium, and cultivate at 35°C for 48 hours; the mass percentage content of each component in the slant seed medium is: 0.5% glucose, 0.2% peptone, 0.5% beef extract, Yeast extract 0.5%, sodium chloride 0.5%, agar 1.5%, the balance is water;

[0020] Step 2. Pick 2-4 rings of bacterial lawn from the freshly cultured slant and inoculate it in a 250ml Erlenmeyer flask equipped with 50ml liquid expansion medium, and cultivate it on a shaker at 35°C and 240rpm for 16h to obtain a seed solution for later use;

[0021] The mass percentage content of each component in the liquid expansion medium is: 0.5% glucose, 0.2% peptone, 0.5% beef extract, 0.5% yeast extract, 0.5% sodium chloride, and the balance is water;

[0022] Step 3, according to the ratio of mass ratio of 2:1:0.2, take Pueraria...

Embodiment 2

[0031] A preparation method of bacillus subtilis traditional Chinese medicine feed additive, comprising the following steps:

[0032] Step 1, inoculate Bacillus subtilis for feed on the slant seed medium, and cultivate at 37° C. for 48 hours; the mass percentage content of each component in the slant seed medium is: glucose 1.5%, peptone 2%, beef extract 1.5%, Yeast extract 1.5%, sodium chloride 0.5%, agar 2%, the balance is water;

[0033] Step 2. Pick 2-4 rings of bacterial lawn from the freshly cultivated slant and inoculate it in a 250ml Erlenmeyer flask equipped with 50ml of liquid expansion medium, shake and culture at 37°C and 240rpm for 24h to obtain seed liquid, and set aside;

[0034] The mass percentage content of each component in the liquid expansion medium is: glucose 1.5%, peptone 2%, beef extract 1.5%, yeast extract 1.5%, sodium chloride 0.5%, and the balance is water;

[0035] Step 3, according to the ratio of mass ratio of 2:1:0.2, take Pueraria root, Rhizom...

Embodiment 3

[0043] A preparation method of bacillus subtilis traditional Chinese medicine feed additive, comprising the following steps:

[0044] Step 1, inoculate Bacillus subtilis for feed on the slant seed medium, and cultivate at 36° C. for 48 hours; the mass percentage content of each component in the slant seed medium is: glucose 1.0%, peptone 1.5%, beef extract 1.0%, Yeast extract 1.2%, sodium chloride 0.5%, agar 1.8%, the balance is water;

[0045] Step 2. Pick 3 rings of bacterial lawn from the cultivated fresh slant and inoculate it in a 250ml Erlenmeyer flask equipped with 50ml of liquid expansion medium, and cultivate it at 36°C and 200rpm on a shaker for 20h to obtain a seed solution for later use;

[0046] The mass percentage content of each component in the liquid expansion medium is: glucose 1.2%, peptone 1.6%, beef extract 1.2%, yeast extract 1.2%, sodium chloride 0.5%, and the balance is water;

[0047] Step 3, according to the ratio of mass ratio of 2:1:0.2, take Puera...

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Abstract

The invention relates to a method for preparing a bacillus subtilis traditional Chinese medicine feed additive. The method comprises the following steps: activating bacillus subtilis for feed, and performing expanded culture so as to obtain a seed solution; performing superfine grinding on kudzu vine roots, polygonatum sibiricum and medical stone, and performing sterilization so as to obtain sterilized traditional Chinese medicine powder A; inoculating the seed solution into a culture liquid, and culturing for 8 hours so as to obtain a bacterial liquid; adding the sterilized traditional Chinese medicine powder A into the bacterial liquid, and continuously culturing so as to obtain a secondary seed solution; performing superfine grinding on dried tangerine or orange peel, houttuynia cordata and hawthorn, and performing sterilization so as to obtain sterilized traditional Chinese medicine powder B; inoculating the secondary seed solution into a liquid fermentation culture medium, and culturing so as to obtain fermentation broth; adding the sterilized traditional Chinese medicine powder B into the fermentation broth, and continuously fermenting for 10-15 hours so as to obtain final fermentation broth; performing vacuum concentration on the final fermentation broth, performing atomization, performing parallel flow drying by using hot air, leading out dried materials by using a cyclone separator, and recycling, thereby obtaining the bacillus subtilis traditional Chinese medicine feed additive. The bacillus subtilis traditional Chinese medicine feed additive is small in water content, good in bacterium activity and wide in market prospect, and the viable count is 700-1800 billion/g.

Description

technical field [0001] The invention relates to a method for preparing bacterial powder, in particular to a method for preparing a bacillus subtilis traditional Chinese medicine feed additive. Background technique [0002] Bacillus subtilis is a commonly used bacterium in microbial preparations. After Bacillus subtilis enters the intestinal tract of animals in the form of spores, it can quickly germinate from a dormant state. , A large amount of free oxygen in the digestive tract is consumed, resulting in an anoxic environment; and harmful bacteria are generally aerobic bacteria, so their growth is inhibited, and anaerobic beneficial bacteria become the dominant flora. At the same time, Bacillus subtilis also has strong protease, amylase and lipase activities, which can activate the zymogen in the body into active enzymes, and can also secrete a series of extracellular enzymes, such as pectinase, dextranase, Cellulase, etc., can help decompose non-starch polysaccharides and...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/12
Inventor 岳占州王新昌安建军杨雪莲岳朝鹏张亚飞
Owner 河南省岳氏精忠科技有限公司
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