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A temperature-controlled phase change material microcapsule carrier immobilized enzyme and its preparation method

A phase change material, the technology of immobilizing enzymes, applied in the direction of being immobilized on or in an inorganic carrier, immobilized on/in an organic carrier, etc., can solve the problem of poor thermal stability and cycle stability, narrow working temperature range, etc. problems, to achieve the effect of increased activity recovery rate, low equipment requirements, and improved stability

Active Publication Date: 2019-03-01
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of narrow working temperature range and poor storage stability, thermal stability and cycle stability of the traditional carrier immobilized enzyme, and provide a kind of immobilized enzyme with temperature-controlled phase change material microcapsule as the carrier material. The enzyme and its preparation method can significantly improve the stability and activity of the immobilized enzyme by utilizing the characteristic of the microenvironment temperature function of the carrier material, and have great application potential in the field of immobilized enzyme development and application

Method used

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  • A temperature-controlled phase change material microcapsule carrier immobilized enzyme and its preparation method
  • A temperature-controlled phase change material microcapsule carrier immobilized enzyme and its preparation method
  • A temperature-controlled phase change material microcapsule carrier immobilized enzyme and its preparation method

Examples

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Effect test

Embodiment 1

[0039] Step (1): Preparation of temperature-controlled phase change material microcapsule carrier

[0040] First, dissolve 1.0g of ferric chloride hexahydrate and 0.4g of ferrous chloride tetrahydrate in 100mL of deionized water, raise the temperature to 60°C for 5 hours, then adjust the pH to 10 with ammonia water, add 0.1g of dodecane Sodium sulfate, continue to stir for 20min under N 2 Prepare hydrophobic magnetic nanoparticles by reacting under the protection of the atmosphere; add 0.4g magnetic nanoparticles, 5.0g n-eicosane and 5.0g n-butyl titanate into a three-necked flask, and ultrasonicate at 40°C for 30min to make the magnetic nanoparticles and Disperse the titanium source on the surface of the organic phase change material, then add 60mL formamide solution and 0.5g sodium lauryl sulfate and continue to stir for 2h to form a stable emulsion system; prepare a mixture of 1mL deionized water and 40mL formamide, and slowly Add dropwise to the above emulsion system, con...

Embodiment example 2

[0044] Step (1): Preparation of temperature-controlled phase change material microcapsule carrier

[0045] First, dissolve 1.0g of ferric chloride hexahydrate and 0.4g of ferrous chloride tetrahydrate in 100mL of deionized water, raise the temperature to 60°C for 5 hours, then adjust the pH to 10 with ammonia water, add 0.1g of dodecane Sodium sulfate, continue to stir for 20min under N 2 Prepare hydrophobic magnetic nanoparticles by reacting under the protection of the atmosphere; add 0.4g magnetic nanoparticles, 5.0g n-eicosane and 5.0g n-butyl titanate into a three-necked flask, and ultrasonicate at 40°C for 30min to make the magnetic nanoparticles and Disperse the titanium source on the surface of the organic phase change material, then add 60mL formamide solution and 0.5g sodium lauryl sulfate and continue to stir for 2h to form a stable emulsion system; prepare a mixture of 1mL deionized water and 40mL formamide, and slowly Add dropwise in the above-mentioned emulsion s...

Embodiment example 3

[0049] Step (1): Preparation of temperature-controlled phase change material microcapsule carrier

[0050] First, dissolve 1.0g of ferric chloride hexahydrate and 0.4g of ferrous chloride tetrahydrate in 100mL of deionized water, raise the temperature to 60°C for 5 hours, then adjust the pH to 10 with ammonia water, add 0.1g of dodecane Sodium sulfate, continue to stir for 20min under N 2 Prepare hydrophobic magnetic nanoparticles by reacting under the protection of the atmosphere; add 0.4g magnetic nanoparticles, 5.0g n-eicosane and 5.0g n-butyl titanate into a three-necked flask, and ultrasonicate at 40°C for 30min to make the magnetic nanoparticles and Disperse the titanium source on the surface of the organic phase change material, then add 60mL formamide solution and 0.5g sodium lauryl sulfate and continue to stir for 2h to form a stable emulsion system; prepare a mixture of 1mL deionized water and 40mL formamide, and slowly Add dropwise to the above emulsion system, con...

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Abstract

The invention discloses a temperature-regulated phase material microcapsule carrier immobilized enzyme and a preparation method for the same, and relates to the technical field of biology. The preparation method comprises the following steps: preparing a microcapsule carrier obtained by cladding an organic phase change material with a crystalline titanium dioxide / magnetic particle composite material, performing carboxylation modification on the microcapsule carrier, and implementing enzyme immobilization by adopting a chemical coupling method, so as to finally obtain the temperature-regulated phase material microcapsule carrier immobilized enzyme. According to the temperature-regulated phase material microcapsule carrier immobilized enzyme and the preparation method for the same, heat can be stored and released by the microcapsule carrier of the immobilized enzyme through a phase change material capsule core, so that micro-environmental temperature around the carrier can be regulated, the temperature application range of enzymatic reaction can be widened, and the heat stability, the storage stability and the cycling stability of the immobilized enzyme can be enhanced. In addition, the temperature-regulated phase change material microcapsule carrier can be used for immobilizing different types of enzymes, and is wide in application range.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a phase change material microcapsule carrier immobilized enzyme with automatic adjustment of microenvironment temperature and a preparation method thereof. Background technique [0002] Enzyme is a macromolecule with catalytic function and is a catalyst for all metabolic reactions in organisms. It has the advantages of mild catalytic reaction conditions, high catalytic efficiency, strong substrate specificity, less by-products, and convenient adjustment of catalytic activity. It is an ideal catalyst. However, the free enzyme has the following disadvantages. Its structure is easily affected by the external environment and inactivation occurs; it is difficult to separate and recover after use, and it is difficult to realize continuous utilization. With the emergence of immobilized enzyme technology, the above-mentioned shortcomings of free enzymes have been overcome, and the applicati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/02
CPCC12N11/02C12N11/14
Inventor 汪晓东蒋彬彬武德珍
Owner BEIJING UNIV OF CHEM TECH
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