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Artificial blood vessel and preparation method thereof

A technology of artificial blood vessels and solutions, applied in blood vessels, devices of human tubular structures, anticoagulant treatment, etc., can solve the problems of inability to prepare implantable artificial blood vessel repair materials, etc., and achieve improved mechanical strength and excellent anticoagulation The effect of the function

Inactive Publication Date: 2016-10-05
刘畅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing engineering techniques, such as mold casting and 3D printing, cannot produce implantable artificial vascular repair materials that can be directly connected to human arteriovenous vessels

Method used

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  • Artificial blood vessel and preparation method thereof
  • Artificial blood vessel and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0034] The invention provides a method for preparing the artificial blood vessel, which is characterized in that the method comprises the following steps:

[0035] 1) First prepare the natural macromolecule solution with a mass percentage of 0.1-20%, and add an anticoagulant factor with a mass percentage of 0.01-1% in the natural macromolecular solution; Add endothelial cells, smooth muscle cells, fibroblasts, stem cells, or a mixture of stem cells and growth factors to prepare a natural polymer solution containing different cells, wherein the density of cells in the natural polymer solution is 1×10 2-7 a / mL;

[0036] 2) Prepare a series of hollow cylindrical molds with different diameters, put a second hollow cylindrical mold with a larger diameter on the outside of the first hollow cylindrical mold, leave a gap between the two molds, and use a dropper or syringe to put the endothelial cells, or The mixture of stem cells and endothelial cells, or the natural polymer solution...

Embodiment 1

[0047] Example 1: A small-diameter blood vessel and its preparation method

[0048] a) Prepare 0.1% sodium alginate and 20% gelatin solutions by mass percentage, mix the sodium alginate and gelatin solutions uniformly at a volume ratio of 1:1, add 1% anticoagulant heparin and 10% Cell cryopreservation factor dimethyl sulfoxide; mix endothelial cells, smooth muscle cells, and fibroblasts with the mixture, and the density of cells in the mixture of sodium alginate and gelatin is 1×10 2 a / mL;

[0049] b) Load various cell-containing solutions in step a) into the nozzle assembly of the 3D printing device, and print layers with a lumen diameter of 0.1mm, which are endothelial cell layer, smooth muscle cell layer and fibroblast cell layer from inside to outside. Hollow cylinder; use 1% calcium chloride solution to cross-link sodium alginate molecules to stabilize the three-dimensional structure;

[0050] c) Spraying a layer of polyethylene solution with a mass volume percentage of 3...

Embodiment 2

[0052] Example 2: A large-diameter blood vessel and its preparation method

[0053] a) Prepare a series of hollow cylindrical molds with a diameter greater than 6mm, respectively prepare fibrinogen solutions containing bone marrow stem cells, endothelial cells and fibroblasts, wherein the mass percentage of the fibrinogen solution is 5%, and the amount of cells in the fibrinogen solution Density is 1×10 7 cells / mL, add 1% smooth muscle cell growth factor, 1% anticoagulant heparin and 10% cell cryopreservation factor dimethyl sulfoxide to the bone marrow stem cell solution;

[0054] b) Put a second hollow cylinder mold with a larger diameter on a first hollow cylinder mold with a smaller diameter, leave a gap in the middle of the two molds, inject the fibrinogen solution containing endothelial cells and heparin into the two molds with a dropper In the gap between the moulds, 10% thrombin is used to polymerize the fibrinogen to form an endothelial cell layer;

[0055] c) Remov...

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Abstract

The invention relates to an artificial blood vessel and a preparation method thereof and belongs to the technical field of organ preparing and biological materials. The artificial blood vessel comprises an outer cortex layer, a fibroblast layer, a smooth muscle cell layer, an endothelial cell layer and an inner cavity. The orderly distribution of the endothelial cell layer, the smooth muscle cell layer, the fibroblast layer and the outer cortex layer in the three-dimensional space is achieved through the comprehensive technology of plasma spraying, electric spraying, electrospinning, reverse molding and 3D printing, the anti-coagulation performance of the artificial blood vessel is enhanced through anti-coagulation factors, step-by-step induced differentiation of stem cells in the artificial blood vessel is achieved through a growth factor controlled-release method, the artificial blood vessel is cultured through a pulsation reactor and made to simulate natural animal blood vessels on the structure and the function, and a corresponding substitute is provided for blood vessel transplanting and repairing. The artificial blood vessel is also applicable to other tubular tissue and organs such as the oesophagus, the trachea, the ovarian duct, the bile duct, the urethra and the bladder.

Description

technical field [0001] The invention relates to an artificial blood vessel and a preparation method thereof, belonging to the technical fields of organ manufacturing and biomaterials. Background technique [0002] According to the statistics of the World Health Organization, cardiovascular diseases are one of the diseases with the highest morbidity and mortality in the world, and more than 17 million people die of heart diseases every year in the world. Cardiovascular diseases have become the largest cause of death in developed countries in Europe, causing at least 2 million deaths each year, accounting for about 40% of the total number of deaths. More than 1.4 million vascular grafts are performed each year in the United States alone. In my country, with the increase of contemporary cardiovascular and cerebrovascular diseases, the incidence of lower extremity venous disease and coronary artery bypass surgery are also increasing year by year. Only Beijing Fuwai Hospital per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/54A61L33/14A61F2/06
CPCA61F2/06A61F2/062A61F2210/0076A61F2240/001A61L27/16A61L27/18A61L27/20A61L27/222A61L27/225A61L27/24A61L27/3804A61L27/3808A61L27/3826A61L27/3891A61L27/507C08L5/08C08L5/04C08L23/06C08L23/12C08L67/04C08L69/00C08L75/04A61L27/3604A61L27/44
Inventor 刘畅
Owner 刘畅
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