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A Strain of Bacillus arborii j5 and Its Application

A technology of Bacillus aristolochia and strains, applied in the field of microbial resources, can solve the problems of limited laboratory and pot experiment stage, etc., and achieve the effect of good pot experiment effect, increase of ear thickness, and huge market application prospect.

Active Publication Date: 2019-05-17
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As a dominant strain of Bacillus arseni as a new microbial agent, the current research is still limited to the stage of laboratory and pot experiments

Method used

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  • A Strain of Bacillus arborii j5 and Its Application
  • A Strain of Bacillus arborii j5 and Its Application
  • A Strain of Bacillus arborii j5 and Its Application

Examples

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Effect test

Embodiment 1

[0038] The present invention will be further described in detail below in conjunction with the accompanying drawings of the specification and specific embodiments. The embodiments are only used to explain the present invention and are not used to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used, unless otherwise specified, are commercially available reagents and materials. Example 1 Isolation of strains

[0039] S1. Separation: Take the vegetable garden soil of Yuejin North Farm of South China Agricultural University, Tianhe District, Guangzhou City, Guangdong Province as the screening soil sample, and accurately weigh 10.0g of the screening soil sample by flat coating method and put it into a 250mL triangle filled with 90mL sterile water In the bottle (add glass beads), shake on a shaker for 30 minutes to fully disperse the cells, and let stand for 20-30 ...

Embodiment 2

[0041] Example 2 Characterization of J5 strain

[0042] Morphological characteristics of the bacteria: Gram-positive bacteria with spores. The bacteria are rod-shaped, sporty, obligate and aerobic. See photos of spore staining figure 1 .

[0043] Colony morphology characteristics: The colonies formed after 24 hours of culture on beef extract peptone medium are round or irregular. After 48 hours, the colonies are all round, slightly yellow, with a diameter of about 2-3mm, with irregular edges, flat and moist.

[0044] Growth characteristics: in a liquid medium of 3.0 g beef extract, 10.0 g peptone, 5.0 g sodium chloride, and 1L water, at a speed of 113 rpm, a temperature of 30°C, and an initial pH value of 7.0, cultured for 18 hours, measured The number of viable bacteria is (1.94±0.60)×10 8 cfu·mL -1 .

[0045] Physiological and biochemical characteristics: Refer to the routine experimental methods of "Berger's Bacteria Identification Manual" to perform some physiological and bioch...

Embodiment 3

[0049] Example 3 J5 bacteria produce long prime effect

[0050] Qualitative determination of auxin: J5 bacteria were inoculated into 50mL LB liquid medium containing L-tryptophan (100 µg / mL), and cultured on a shaker at 30°C and 150r / min for 24h. Take 300 μL of culture solution drop on the white ceramic plate, and add 300 μL of Salkowski colorimetric solution at the same time, set 300μL of sterile LB medium as a negative control, 10, 20, 30, 50 (μg / mL) auxin colorimetric solution As a positive control. Place the white ceramic plate at room temperature and protected from light for 30 minutes and observe. If the color turns red, it means that long pigment can be produced. See the test results Figure 4 .

[0051] Quantitative determination of auxin: J5 bacteria were inoculated into 50mL LB liquid medium containing L-tryptophan (100 µg / mL), and cultured in three replicates at 30°C and 150r / min shaker for 24h. Centrifuge the culture solution at 8000×g for 10 min, add 4 mL of the sup...

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Abstract

The invention belongs to the field of microbial resources, and specifically discloses a strain of Bacillus aristolochia J5 and its application in corn growth promotion. Bacillus alba J5 was deposited in China General Microorganism Culture Collection and Management Center (CGMCC) on October 15, 2015, and the deposit number is CGMCC No.11508. The Bacillus adenii J5 of the present invention has the functions of auxin production, siderophore production, ammonia production, protease production, salt tolerance, antagonism and phosphate solubilization; the strain grows normally under the condition of the medium containing 11% of salt, showing It has a good potential to eliminate soil salinization; the content of siderophore reaches 53.7%, which has high disease prevention characteristics. The strain can improve the germination rate of corn seeds by 12.5%, and has a good effect of promoting growth. After the inoculum prepared by the strain was applied, the yield of corn was increased by 37.1%, and the 100-grain weight was increased by 35%. The strain is expected to be developed as a biological inoculant, and has broad application prospects in improving soil nutrients, eliminating salinization, promoting plant growth and preventing diseases.

Description

Technical field [0001] The present invention relates to the field of microbial resources, and more specifically, to a strain of Bacillus Ascheri J5 and its application. Background technique [0002] Plant growth-promoting rhizobacteria (PGPR) refers to microorganisms that live in the rhizosphere and root surface of plants and can directly or indirectly promote or regulate plant growth. Plant rhizosphere growth-promoting bacteria generally have the ability to produce long hormone, ammonia, siderophore, antagonism, phosphorous solution, salt tolerance, etc. or at least one of them. Inoculation of plant rhizosphere growth-promoting bacteria can make soil nutrients effective and promote plant roots to absorb water and nutrients in the soil to improve plant growth. Bacillus produces spores that are highly resistant to heat, ionizing radiation and certain chemicals. Therefore, the bacillus inocula with rhizosphere growth promoting effect has simple production process, long shelf life...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 蔡燕飞曹禺李永涛江亚雄王玉琪王琰张慧敏
Owner SOUTH CHINA AGRI UNIV
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