Special complex enzyme system for kelp comprehensive utilization and application thereof

A composite enzyme system and composite enzyme technology, applied in the field of composite enzyme systems, can solve the problems of immature technical system, low application efficiency, and poor extraction effect of functional products, so as to improve the comprehensive utilization rate and reduce the use of acid and alkali The effect of improving food safety and environmental protection

Inactive Publication Date: 2016-10-12
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current shortcomings of low application efficiency of biological enzyme preparations in the field of kelp processing, poor extraction effect of functional products, and immature technical system, the present invention solves the above problems through a large number of researches, and provides a method that can simultaneously extract fucoidan, High-efficiency compound enzyme system for comprehensive utilization of kelp with four functional products of alginic acid, mannitol and kelp dietary fiber

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A special composite enzyme system for the comprehensive utilization of kelp that can simultaneously extract fucoidan, alginic acid, mannitol and kelp dietary fiber, including enzyme A and enzyme B, the enzyme A is composed of the following components by mass fraction: cellulose 40% enzyme, 30% hemicellulase, 25% pectinase, 0.5% amylase, 4% xylanase, 0.5% pentosanase; the enzyme B is composed of the following components by mass fraction: Alkaline protease 80%, papain 20%. The enzyme activity of the components in the enzyme A is as follows: cellulase: 500,000 U / g; hemicellulase: 100,000 U / g; pectinase: 300,000 U / g; amylase: 10,000 U / g; Xylanase: 100,000 U / g; Pentosanase: 100,000 U / g. The enzyme activities of the components in the enzyme B are as follows: alkaline protease: 500,000 U / g; papain: 800,000 U / g.

[0032] The application method of the compound enzyme system is as follows:

[0033] (1) Using fresh kelp as raw material, cut the kelp into 5-7cm kelp pieces, add ...

Embodiment 2

[0040] A special composite enzyme system for the comprehensive utilization of kelp that can simultaneously extract fucoidan, alginic acid, mannitol and kelp dietary fiber, including enzyme A and enzyme B, the enzyme A is composed of the following components by mass fraction: cellulose 30% enzyme, 50% hemicellulase, 1% amylase, 14% xylanase, 5% β-glucanase; the enzyme B is composed of the following components in mass fraction: alkaline protease 70% %, neutral protease 30%. The enzyme activity of the components in the enzyme A is as follows: cellulase: 500,000 U / g; hemicellulase: 100,000 U / g; amylase: 10,000 U / g; xylanase: 100,000 U / g ; β-glucanase: 200,000 U / g. The enzyme activities of the components in the enzyme B are as follows: alkaline protease: 500,000 U / g; neutral protease: 100,000 U / g.

[0041] The application method of the compound enzyme system is as follows:

[0042] (1) Using dried kelp as raw material, cut the kelp into kelp pieces of 2-5 cm, add 5-10 times the ...

Embodiment 3

[0049] A special composite enzyme system for the comprehensive utilization of kelp that can simultaneously extract fucoidan, alginic acid, mannitol and kelp dietary fiber, including enzyme A and enzyme B, the enzyme A is composed of the following components by mass fraction: cellulose Enzyme 25%, cellulase 45%, pectinase 14.5%, amylase 0.5%, xylanase 15%; the enzyme B is composed of the following components in mass fraction: acid protease 100%. The enzyme activity of the components in the enzyme A is as follows: cellulase: 500,000 U / g; hemicellulase: 100,000 U / g; pectinase: 300,000 U / g; amylase: 10,000 U / g; Xylanase: 100,000 U / g. The enzyme activities of the components in the enzyme B are as follows: acid protease: 800,000 U / g.

[0050] The application method of the compound enzyme system is as follows:

[0051] (1) Use kelp slag after extracting iodine or mannitol as raw material, add 5 to 7 times of water, adjust the pH to 4.8, add 0.15% of compound enzyme A (by dry weight...

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PUM

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Abstract

The invention discloses a special complex enzyme system for kelp comprehensive utilization and application thereof. The complex enzyme system comprises an enzyme A and an enzyme B, wherein the enzyme A comprises any four or more than four kinds of materials from cellulose, hemicellulase, pectinase, amylase, xylanase, alkaline cellulose, pentosanase, glucanase and feruloyl esterase; the enzyme B comprises one or any two kinds of materials from alkaline protease, neutral protease, papain, trypsin, acid proteinase and pepsin. The complex enzyme system has the prominent beneficial effects that after the complex enzyme system is used, four kinds of products of fucoidin, alginic acid, mannitol and functional dietary fiber can be simultaneously extracted from kelps; the extraction ratio of four kinds of active ingredients reaches more than 90 percent (through being metered by the kelp dry weight); the comprehensive utilization rate of kelp resources is obviously improved; the product additional values of kelp processing industry are greatly increased; the technological level of relative products is improved.

Description

technical field [0001] The invention relates to a compound enzyme system specially used for the comprehensive utilization of kelp and its application, and belongs to the technical field of deep processing of marine resources. Background technique [0002] Kelp is a brown algae plant that can be used for both food and medicine. It is rich in nutrition and contains a variety of active ingredients that are beneficial to the human body, such as iodine, mannitol, alginic acid, fucoidan, dietary fiber, multivitamins, calcium, iron, etc. . my country is a big country for kelp resources and comprehensive utilization. Kelp is a very important marine plant resource in my country and one of the main raw materials for the brown algae industry. At present, a brown algae industrial product system with alginic acid, mannitol, and iodine as the main products has been formed. But because my country's current kelp processing industry is mainly based on rough processing, intensive processing ...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/24C12N9/88C12N9/26C12N9/46C12N9/18C12N9/50C12N9/76C12N9/64C12P19/16C12P19/14C12P19/04C07C29/74C07C31/26A23L33/21
CPCC12N9/2437A23V2002/00C07C29/74C12N9/18C12N9/2402C12N9/2411C12N9/2445C12N9/2454C12N9/2477C12N9/248C12N9/2482C12N9/50C12N9/63C12N9/6427C12N9/6481C12N9/88C12P19/04C12P19/14C12P19/16C12Y301/01073C12Y302/01004C12Y302/01008C12Y302/01011C12Y302/01015C12Y302/01021C12Y302/01067C12Y302/01091C12Y302/01099C12Y304/21004C12Y304/22002C12Y402/0201A23V2250/202A23V2250/5116C07C31/26
Inventor 董学前赵林张永刚王伟杨晓雪张艳敏吉武科刘建军
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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