Preparation method of natural para aminobenzoic acid and derivative of natural para aminobenzoic acid

A technology of para-aminobenzoic acid and derivatives, applied in the field of biomedicine, can solve problems such as environmental pollution, and achieve the effects of being environmentally friendly, having a wide range of sources and being easy to industrialize production.

Active Publication Date: 2016-10-12
FUJIAN NORMAL UNIV
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  • Claims
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Problems solved by technology

Chemical synthesis often uses a large amount of chemical reagents, which will cause environmental pollution

Method used

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  • Preparation method of natural para aminobenzoic acid and derivative of natural para aminobenzoic acid
  • Preparation method of natural para aminobenzoic acid and derivative of natural para aminobenzoic acid
  • Preparation method of natural para aminobenzoic acid and derivative of natural para aminobenzoic acid

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preparation example Construction

[0014] The preparation method of natural p-aminobenzoic acid and derivative thereof provided by the invention, its steps are:

[0015] 1) Liquid culture and fermentation product processing: the fungus Termitomyces albuminosus The slant strains were first inoculated in a Erlenmeyer flask containing 150mL potato liquid medium for activation. The activation conditions were 230 r / min, 28°C, and cultured for 7 days. Then, large-scale liquid fermentation was carried out. The medium formula was: : Each liter of water contains 200 g of potatoes, 20 g of glucose, natural pH, 0.1 Mpa, sterilized at 121 ℃ for 30 minutes, and cultured in a constant temperature shaker at 25-28 ℃ and 180-230 r / min. After 15 days of fermentation, the mycelium was separated from the fermentation broth by centrifugation. The fermented liquid was extracted with an equal volume of ethyl acetate, the obtained extract was dehydrated with anhydrous sodium sulfate, and then concentrated in vacuo with a rotary evapo...

Embodiment 1

[0019] Use potato glucose medium (containing 200g of potato per liter of water, 20g of glucose, natural pH, 0.1Mpa, sterilized at 121°C for 30min), and the activated strain Termitomyces albuminosus 37 L of liquid fermentation culture was cultured on a shaker at 28 °C for 15 days. After the bacterial cells were filtered, the fermentation broth was concentrated to 1 L, and the fermentation broth was extracted with ethyl acetate. The ethyl acetate part was dehydrated with anhydrous sodium sulfate and then concentrated to obtain an organic crude extract (3.1 g).

[0020] The 3.1 g organic crude extract obtained in the previous step was fully dissolved in methanol, and subjected to reverse-phase silica gel (170 g) column chromatography, and eluted with 30% methanol water for 2 L at a flow rate of 15 mL / min. Collect 280 mL, concentrate in vacuo with a rotary evaporator, take samples separately for thin-layer chromatography analysis and combine (developing solvent is chloroform:metha...

Embodiment 2

[0027] Use potato glucose medium (containing 200g of potato per liter of water, 20g of glucose, natural pH, 0.1Mpa, sterilized at 121°C for 30min), and the activated strain Termitomyces albuminosus 37 L of liquid fermentation culture was cultured on a shaker at 28 °C for 15 days. After the bacterial cells were filtered, the fermentation broth was concentrated to 1 L, and the fermentation broth was extracted with ethyl acetate. The ethyl acetate part was dehydrated with anhydrous sodium sulfate and then concentrated to obtain an organic crude extract (3.1 g).

[0028] Dissolve 3.1 g of the crude organic extract obtained in the previous step in methanol, perform reverse phase silica gel (170 g) column chromatography, and elute 2 L with 30% methanol water at a flow rate of 15 mL / min. Collect 280 mL, concentrate in vacuo with a rotary evaporator, take samples in separate tubes for thin-layer chromatography detection and combine (developing solvent is chloroform:methanol=10:1, chro...

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Abstract

The invention relates to a preparation method of natural para aminobenzoic acid and a derivative of natural para aminobenzoic acid. Termitomyces albuminosus is cultured through liquid fermentation, a fermentation product is obtained, and then natural para aminobenzoic acid and the derivative of natural para aminobenzoic acid are separated and purified from the fermentation product. With the adoption of the preparation method of natural para aminobenzoic acid and the derivative of natural para aminobenzoic acid, natural para aminobenzoic acid and the derivative of natural para aminobenzoic acid can be prepared by the aid of the fungus termitomyces albuminosus through liquid fermentation, sources of raw materials of a culture medium are wide, the preparation method is simple, safe and environment-friendly, and industrial production is easy to realize.

Description

technical field [0001] The invention relates to a preparation method of natural p-aminobenzoic acid and derivatives thereof, belonging to the field of biomedicine. Background technique [0002] p-Aminobenzoic acid, also known as PABA, etc., is an important precursor and intermediate in many organic synthesis, used in dyes and pharmaceutical intermediates, can be used to produce reactive red M-80, M-10B, reactive red violet X-2R and other dyes and the production of cyanobenzoic acid to produce p-carboxybenzylamine. P-aminobenzoic acid can be used as a sunscreen, and its derivative, octyl p-dimethylcarbamate, is an excellent sunscreen. Some derivatives containing PABA structural units have anti-cancer, anti-tumor, anti-hepatitis B virus and other pharmacological effects. PABA can also participate in the synthesis of folic acid, and its amino acid derivatives can be used in the synthesis of dihydrofolate reductase, thymidylate synthase, farnesyl transferase and other enzyme i...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12R1/645
CPCC12P13/005
Inventor 郑永标吴雅滨邹先文
Owner FUJIAN NORMAL UNIV
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