Lepista nuda culture medium based on yellow wine lees and preparation method of lepista nuda liquid strain

A technology of lilac mushroom and liquid strain, which is applied in the preparation of lilac mushroom liquid strain and the field of lilac mushroom culture medium, can solve the problems of large strain damage, less mycelium, and weak penetrating power, etc., and achieve The effect of low price cost, uniform distribution and suitable size

Inactive Publication Date: 2016-10-26
安徽天明生态林农科技开发有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating power is not strong. If the compost is too thick, mycelium is not easy to penetrate the compost, and the mycelia will follow the compost. The surface grows rapidly, and the amount of mycelium inside the compost is very small, which seriously affects the yield and quality of the strain; 2. Although the conventional liquid strain solves some problems existing in the solid strain, it can significantly shorten the cultivation cycle and enhance the growth rate of the strain. Species vigor and stress resistance, reducing the contamination rate of strains, and improving the uniformity of strain quality, but there are still some deficiencies such as the density, size, and uniformity of the bacterial balls in the liquid strains obtained by fermentation are not very ideal. If the bacteria balls are crushed by stirring and shearing, the uniformity of the obtained strains will be more ideal. However, the shear force of high-speed stirring, crushing and beating will greatly damage the strains and have a serious impact on the overall quality of the strains.
Publication No. CN105309194A "New Technology for the Production of Edible Mushroom Microparticle Liquid Strain" provides a kind of edible fungus microparticle liquid strain with a mycelium ball particle diameter of 0.5mm, which increases the ventilation rate of the edible fungus liquid strain culture solution, so that the ventilation rate It is suitable at 1:0.6—0.9V / V·min. At the same time, a stirrer is installed in the aerated fermenter to make the eddy current of the culture liquid move and prolong the technical operation method of the air bubble. The method for producing the microparticle liquid strain is from the production process There is no improvement in the aspect of liquid culture medium, and the implementation is technically difficult and demanding; Publication No. CN105130516A "Method for Making Molecular-level Biological Medium" mentions that the nutrients of molecular-level biological medium can be absorbed quickly and comprehensively. Promotes the growth of liquid strains, but does not mention the effect of molecular-grade biological media on the diameter of liquid strain mycelium balls

Method used

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Embodiment Construction

[0014] A lilac mushroom culture medium based on yellow distiller's grains, comprising the following components in parts by weight: 140 parts by weight of rice distiller's grains, 40 mint leaves, 3 onion extracts, 120 enzymatically hydrolyzed fish scale powder, 9 molasses, 1 sodium naphthalene acetate, and yeast extract 4. Soybean isoflavones 1.5, zinc lactate 0.9, adenine 0.8, defoaming oil 1, magnesium sulfate 1.2, gypsum 0.6, potassium dihydrogen phosphate 0.5, superphosphate 0.7, nano tourmaline powder 5.6, bacterial nanocellulose 6.2, Cellulase 3, glucoamylase 2.8, appropriate amount of water.

[0015] A kind of method adopting above-mentioned culture medium to prepare lilac mushroom liquid spawn, comprises the following steps:

[0016] (1) Mix nano-scale tourmaline powder and water at 0.1g / L, and electrify to prepare sterile water for use; mix the total amount of defoaming oil, gypsum, magnesium sulfate, potassium dihydrogen phosphate, superphosphate and sterile Mix and ...

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Abstract

The invention discloses a lepista nuda culture medium based on yellow wine lees. The lepista nuda culture medium is prepared from, by weight, the yellow wine lees, mint leaves, onion extract, enzymatically-hydrolyzed fish scale powder, yeast extract, soy isoflavone, zinc lactate, adenine, nanoscale tourmaline powder, bacterial nano cellulose and the like. The culture medium is prepared from the yellow wine lees, the mint leaves, the enzymatically-hydrolyzed fish scale powder, molasses and the like and used for preparing a lepista nuda liquid strain, the adopted culture medium material is low in price cost, certain fragrance is provided for the culture medium through treatment of the raw materials, and a certain effect is achieved for improving the fragrance of lepista nuda; meanwhile, nutrient substances of the bacterial nano cellulose with the infectious microbe restraining effect are added into the culture medium, the nutrient substances provide good growth and development space for lepista nuda liquid strain mycelium pellets, along with degradation and absorption use of the bacterial nano cellulose, the onion extract with the infectious microbe restraining effect exerts effects, and a good environment is provided for lepista nuda fruiting bodies.

Description

technical field [0001] The invention relates to the technical field of liquid strains, in particular to a culture medium for lilac mushrooms based on rice distiller's grains and a preparation method for liquid strains of lilac mushrooms. Background technique [0002] In the production process of Lilac mushroom, liquid strains have incomparable advantages over solid strains. Liquid strains have short production cycle, short and consistent bacterial age, high purity, strong vitality, simple and quick inoculation, and easy implementation of industrialization and large-scale production. The advantages of modernization and standardized production. Now, more and more people are researching and using liquid strains, and the promotion and use of liquid strains is also an inevitable trend to realize industrialization, scale and standardization of edible fungi. However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 储荣生
Owner 安徽天明生态林农科技开发有限公司
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