Preparation method of exogenous easily extractable glomalin soil conditioner and use method thereof
The technology of soil conditioner and glocycin, which is applied in the agricultural field, can solve the problems of single preparation raw material, unstable product quality, effect and performance, affecting soil environment and water environment, etc., so as not to pollute the environment and promote soil nutrients. Recycling and eco-economic benefits have obvious effects
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[0015] The preparation method of a kind of exogenous easy-to-extract glomycin soil conditioner of the present invention comprises the following steps:
[0016] 1), the preparation of sodium citrate extraction agent: adopt citric acid solution and disodium hydrogen phosphate solution to mix uniformly with the ratio of 13:87 and obtain sodium citrate extraction agent;
[0017] 2) Preparation of exogenous exogenous exogenous glomycin soil conditioner: add air-dried soil to sodium citrate extraction agent, sterilize, centrifuge and collect supernatant to obtain glomycin solution.
[0018] 3) The solution prepared in step 2) is diluted with sodium citrate buffer solution, which is the exogenous glomycin soil conditioner that is easily extracted.
[0019] Preferably, in step 1), the preparation of the citric acid solution is: take 0.42~4.2g C 6 h 8 o 7 ·H 2 O was dissolved in 100-1000mL distilled water to prepare a 0.02mol / L citric acid solution;
[0020] Preferably, in step 1)...
Embodiment 1
[0031] 1. Preparation of exogenous easy-to-extract glomycin
[0032] The 0-20cm soil (yellow soil) was collected in the citrus orchard of Yangtze University, mixed evenly, air-dried, and passed through a 4mm sieve to remove roots, garbage and stones in the soil. Then extract the exogenous easy-to-extract glomycin according to the following method:
[0033] 1), the preparation of sodium citrate extractant:
[0034] The preparation of citric acid solution is: take 4.2g C 6 h 8 o 7 ·H 2 O was dissolved in 1000mL distilled water to prepare a 0.02mol / L citric acid solution;
[0035] The preparation of disodium hydrogen phosphate solution is: take 5.365g Na 2 HPO 4 ·7H 2 O was dissolved to 1000mL to prepare a 0.02mol / L disodium hydrogen phosphate solution;
[0036] Get 26g citric acid solution phosphorus, 174g disodium hydrogenate solution and mix to obtain the sodium citrate extractant of 20mmol / L (pH=7.0) respectively;
[0037] 2), take 25g of air-dried soil samples in a...
Embodiment 2
[0074] 1. Preparation of exogenous easy-to-extract glomycin
[0075] Preparation method is the same as example 1
[0076] 2. Materials and methods
[0077] The experiment was carried out in 2014 at No. 1 Wenzhou tangerine tree in Zhizhen National Day in the Orangery of the West Campus of Changjiang University in Jingzhou City. The experiment was designed as a single factor experiment. A total of 2 treatments, including:
[0078] 1) Zero-intensity exogenous easy-to-extract glomycin solution as a control (denoted as 0): 500mL zero-intensity exogenous easy-to-extract glomycin extract, watering treatment on the east and west sides within the drip line of citrus trees;
[0079] 2) 1 / 2 strength of exogenous easy-to-extract glomycin solution (denoted as 1 / 2): 500mL 1 / 2 strength EE-GRSP extract, watering treatment on the east and west sides within the drip line of citrus trees.
[0080] Each treatment was repeated 4 times, each tree was 1 plot, a total of 8 trees were randomly arr...
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