Method for conducting bioleaching treatment on heavy metal contaminated soil through composite filamentous fungi by taking straw as carbon source

A filamentous fungus, contaminated soil technology, applied in the restoration of contaminated soil, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of high treatment cost, limited, single maximum acid production capacity, etc. The effect of low consumption and less reaction equipment

Active Publication Date: 2016-12-07
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the relatively single type of acid production of a single bacteria and the limited maximum acid production capacity (in most cases, it can only reduce the soil pH to about pH3.5), it is often impossible to ensure the different types and forms of soil after bioleaching treatment. Heavy metals can be removed in time and effectively (for example, the effective removal of Pb, Cd, and Ni generally requires pH<2.5)
In addition, adding a large amount of commercial organic carbon sources also has the disadvantage of high processing costs

Method used

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  • Method for conducting bioleaching treatment on heavy metal contaminated soil through composite filamentous fungi by taking straw as carbon source
  • Method for conducting bioleaching treatment on heavy metal contaminated soil through composite filamentous fungi by taking straw as carbon source
  • Method for conducting bioleaching treatment on heavy metal contaminated soil through composite filamentous fungi by taking straw as carbon source

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Isolation and cultivation of embodiment 1 Penicillium simplicissimum NAU-12 and Aspergillus niger A80:

[0038] Weigh 10g of soil collected from a copper mine in Tongling, Anhui Province, pour it into a triangular flask filled with a certain volume of sterile water, at 28°C and 120r / min, shake and cultivate for 1 day, then draw 2mL of bacterial suspension from the triangular flask coated on solid growth medium (NaNO 3 : 1.5g / L, K 2 HPO 4 : 0.5g / L, KCl: 0.025g / L, MgSO 4 : 0.025g / L, yeast extract: 1.5g / L, sucrose: 35g / L, agar: 20g / L), cultured at 28°C for 4 days. Pick the suspected strains with well-developed hyphae and streak them on the solid growth medium containing 3% bromocresol green (acid production indicator). Two times, two purified strains were obtained, named NAU-12 and A80 respectively. Referring to the "Handbook of Fungal Identification" and "Mycology of China", the strains NAU-12 and A80 were initially identified as Penicillium and Aspergillus, respecti...

Embodiment 2

[0043] Embodiment 2 handles single heavy metal Cu polluted soil

[0044] Composite filamentous fungi bioleaching process the method of Cu-contaminated soil, the steps are as follows:

[0045] Step (1), preparation of bacteria agent: according to the method of embodiment 1, obtain bacterial density respectively and be 10 8 Spores / mL of NAU-12 and 10 7 The A80 microbial agent of spore / mL is mixed in a volume ratio of 1:1 to obtain a composite bacterial agent for inoculation;

[0046] Step (2), preparing wheat straw carbon source: mechanically pulverize air-dried wheat straw (total solid content 92.5%) to 1 cm, soak in 1.5% NaOH solution at 80° C. for 2 hours for alkali-heat pretreatment, and set aside;

[0047] Step (3), biological removal of Cu from soil: the soil sample was collected from a copper mining area in Tongling, Anhui Province, the soil sample was yellow-red soil, and the soil pH value was 6.22. After testing, the soil sample was mainly polluted by a single Cu, an...

Embodiment 3

[0055] Embodiment 3 handles composite heavy metal Pb-Zn-Cd polluted soil

[0056] Composite filamentous fungus bioleaching process composite heavy metal Pb-Zn-Cd contaminated soil method, the steps are as follows:

[0057] Step (1), preparation of bacterial agent: with embodiment 2;

[0058] Step (2), preparing rice straw carbon source: mechanically pulverize the air-dried rice straw (total solid content 90.2%) to 1 cm, soak in 1% NaOH solution at 100° C. for 1.5 h, carry out alkali-heat pretreatment, and set aside;

[0059] Step (3), biological removal of Zn, Pb and Cd in the soil: the soil sample was collected from the polluted soil around a lead-zinc mine in Dexing, Jiangxi. The soil sample was Quaternary red soil with a pH value of 5.7. After testing, the contents of Zn, Pb and Cd in the soil sample were 1520mg / kg, 570mg / kg and 28mg / kg respectively, exceeding the national soil environmental quality standard (GB15618-1995).

[0060] Get 250g of Pb-Zn-Cd composite contamin...

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Abstract

The invention discloses a filamentous fungi strain NAU-12 which is classified and named as Penicillium simplicissiumum and preserved in the China General Microbiological Culture Collection Center on June 25, 2015, and the preservation number is CGMCC NO.10990. The invention further discloses a composite filamentous fungal inoculant formed by mixing isometric Penicillium simplicissiumum NAU-12 bacterium (1*10<8> spores/mL-2*10<8> spores/mL) and aspergillus niger A80 bacterium (1*10<7> spores/mL-2*10<7> spores/mL). The invention further discloses a method for conducting bioleaching treatment on heavy metal contaminated soil through composite filamentous fungi which grow rapidly in soil, high in heavy metal resistance and capable of utilizing straw subjected to alkali-heat pretreatment as a growth carbon source and highly produce multiple small molecular organic acids. The method is adopted for treating the heavy metal contaminated soil, the removal efficiency of heavy metal is high , and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering, and relates to a method for treating heavy metal-contaminated soil by bioleaching of composite filamentous fungi using straw as a carbon source. Waste straw is used as a carbon source for growth, and composite filamentous fungi (Penicillium simpleum and Aspergillus niger) that produce a variety of small molecular organic acids are an efficient and low-cost method for removing heavy metals in soil. Background technique [0002] High-speed industrialization, urbanization and agricultural intensification have led to increasingly serious soil heavy metal pollution. According to the calculations of the Ministry of Agriculture, the area of ​​cultivated land polluted by heavy metals such as Pb, Cd, and Cu in my country has reached 10 million hectares at present, and the survey by China Environmental Monitoring Center has approached 20 million hectares, and the grain contaminated by hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/80C12R1/685
CPCB09C1/105C12N1/14C12N1/145C12R2001/685C12R2001/80
Inventor 方迪周立祥李洁陶能
Owner NANJING AGRICULTURAL UNIVERSITY
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