Aromatic heterocyclic derivatives and their application in medicine
A drug and pharmacy technology, applied in the field of medicine, which can solve the problems of limited dose and low therapeutic ratio.
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Embodiment 1
[0476]Example 1: ((3R,5S)-1-(5-((S)-1-aminoethyl)-2-(3-(cyclopropylmethoxy)-4-(difluoromethoxy )phenyl)oxazole-4-carbonyl)-5-(hydroxymethyl)pyrrolidin-3-yl)methyl carbamate hydrochloride
[0477]
[0478] Step 1: Synthesis of compound (2S,4R)-2-methoxycarbonyl-4-((methoxycarbonyl)amino)pyrrolidine hydrochloride
[0479] N-tert-butoxycarbonyl-cis-4-hydroxy-L-proline methyl ester (210mg, 0.86mmol), p-toluenesulfonyl chloride (195mg, 1.03mmol), triethylamine (200mg, 2.14mmol ) and 4-dimethylaminopyridine (12mg, 0.086mmol) were dissolved in dichloromethane (10mL), reacted at room temperature for 10h, then stopped the reaction, added water (20mL), extracted with dichloromethane (10mL×3), combined the organic phases with Anhydrous Na 2 SO 4 Drying, removal of solvent, and the concentrated solution was subjected to column chromatography (eluent: Petroleumether / EtOAc (v / v)=4 / 1) to obtain 110 g of a colorless liquid: (2S,4S)-2-methoxycarbonyl-4 - tert-butyl p-toluenesulfonyloxyp...
Embodiment 2
[0509] Example 2: 4-(((2S,4R)-1-(5-((S)-1-aminoethyl)-2-(3-(cyclopropylmethoxy)-4-(difluoro Methoxy)phenyl)oxazole-4-carbonyl)-4-((methoxycarbonyl)amino)pyrrolidin-2-yl)methoxy)benzoic acid hydrochloride
[0510]
[0511] Step 1: Compound 4-(((2S,4R)-1-(5-((S)-1-((tert-butoxycarbonyl)amino)ethyl)-2-(3-(cyclopropylmethoxy Synthesis of methyl)-4-(difluoromethoxy)phenyl)oxazole-4-carbonyl)-4-((methoxycarbonyl)amino)pyrrolidin-2-yl)methoxy)benzoate
[0512] The compound ((3R,5S)-1-(5-((S)-1-((tert-butoxycarbonyl)amino)ethyl)-2-(3-(cyclopropylmethoxy)-4 -(Difluoromethoxy)phenyl)oxazole-4-carbonyl)-5-(hydroxymethyl)pyrrolidin-3-yl)methyl carbamate (200 mg, 0.32 mmol) was dissolved in THF (10 mL), Add methyl 4-hydroxybenzoate (85mg, 0.55mmol) and triphenylphosphine (126mg, 0.48mmol), add diisopropyl azodicarboxylate (DIAD) (97mg, 0.48mmol) in an ice bath, and react at room temperature Stop the reaction after 12h, add saturated ammonium chloride aqueous solution (10mL), extract ...
Embodiment 3
[0523] Example 3: ((3R,5S)-1-(5-((S)-1-amino-2-methylpropyl)-2-(3-(cyclopropylmethoxy)-4-( Difluoromethoxy)phenyl)oxazole-4-carbonyl)-5-(hydroxymethyl)pyrrolidin-3-yl)methyl carbamate hydrochloride
[0524]
[0525] Step 1: Compound (S)-5-(1-((tert-butoxycarbonyl)amino)-2-methylpropyl)-2-(3-(cyclopropylmethoxy)-4-(di Synthesis of methyl fluoromethoxy)phenyl)oxazole-4-carboxylate
[0526] At room temperature, N,N'-carbonyldiimidazole (CDI) (1.20g, 7.5mmol) was dissolved in anhydrous THF (10mL), and compound (S)-2-((tert-butoxycarbonyl)amino) was added slowly -3-Methylbutanoic acid (1.4g, 6.3mmol), stirred at room temperature for 20min, added 2-(((3-(cyclopropylmethoxy)-4-(difluoromethoxy)phenyl)(methyl Thio)methylene)amino)methyl acetate (1.5g, 4.2mmol), at -78°C, slowly add a THF solution (1.0M, 13mL) of potassium hexamethyldisilazide dropwise, and react for 1.5 Stop after h, add water (25mL) to quench the reaction, then extract with ethyl acetate (25mL×3), combine the o...
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