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Novel reagent for detecting blood total bilirubin through synthetic chemistry oxidation method

A technology of chemical oxidation and total bilirubin, applied in measuring devices, scientific instruments, biological testing, etc., can solve problems such as expensive, unstable diazo reagents, and inaccurate results, so as to improve specificity and ensure accuracy Effect

Inactive Publication Date: 2017-01-25
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are diazo reagent method, HPLC method, direct method, ELISA immunoassay, multi-layer dry film method, enzymatic method and chemical oxidation method to detect blood total bilirubin, among which the three most common methods are diazo reagent method, enzymatic method and chemical oxidation method, the diazonium method is a recognized detection method, but its shortcomings are also very obvious, its diazo reagent is unstable, and after many optimizations, the result has not been very ideal; However, because the bilirubin oxidase used in the reagents is mostly imported, the price is expensive, which increases the cost of use; the chemical method entered our country in the 1990s, and in recent years, the domestic chemical There are also many researches on oxidation methods, most of which use vanadate oxidation method or nitrite oxidation method, but the accuracy and specificity of these two methods are highly controversial, often leading to inaccurate results, and most of the reagents are not resistant to heparin Therefore, a new type of chemical oxidation method was invented to detect blood total bilirubin for these problems.

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  • Novel reagent for detecting blood total bilirubin through synthetic chemistry oxidation method
  • Novel reagent for detecting blood total bilirubin through synthetic chemistry oxidation method
  • Novel reagent for detecting blood total bilirubin through synthetic chemistry oxidation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A recognized assay reagent scheme for enzymatic detection of total blood bilirubin:

[0048] 1) Reagent R1:

[0049] Tris-HCL PH=8.2 100mmol / L

[0050] Sodium cholate 4mmol / L

[0051] SDS 15mmol / L

[0052] Reagent R2:

[0053] TRIS-HCL PH=8.2 100mmol / L

[0054] Bovine serum albumin 1g / L

[0055] Bilirubin oxidase 4KU / L.

[0056] The usage method of this embodiment reagent:

[0057] The oxidase method total bilirubin detection reagent described in this embodiment adopts a full-automatic biochemical analyzer with dual reagent functions during use, such as Hitachi 7180 full-automatic analyzer, etc., and utilizes the two-point endpoint method to measure; R1 and R2 is placed on the corresponding reagent position according to the ratio of 3:1, and the distilled water, standard and sample are placed on the corresponding position of the sample plate. The operation is as follows: image 3 .

Embodiment 2

[0059] A novel comprehensive chemical oxidation method detection reagent for total blood bilirubin, characterized in that it includes reagent R1 and reagent R2,

[0060] Reagent R1:

[0061] Potassium hydrogen phthalate buffer 100mmol / L

[0062] Dodecyltrimethylammonium bromide 20mmol / L

[0063] Alkyl glucoside 3ml / L

[0064] Triton-405 3ml / L

[0065] PC-300 0.5ml / L

[0066] Sodium sulfate 5g / L

[0067] Reagent R2:

[0068] Phosphate buffer 10mmol / L

[0069] Sodium perbenzoate 4mmol / L

[0070] Sodium persulfate 2mmol / L

[0071] Disodium EDTA 20g / L

[0072] PC-300 0.5ml / L

[0073] Triton-405 3ml / L.

[0074]The usage method of this embodiment reagent:

[0075] The HCY detection reagent described in this embodiment adopts a full-automatic biochemical analyzer with dual reagent functions during use, such as Hitachi 7180 full-automatic analyzer, etc., and uses the two-point endpoint method to measure; R1 and R2 are determined according to the ratio of 4:1 Place the rat...

Embodiment 3

[0077] This example describes a new comprehensive chemical oxidation method for detecting total blood bilirubin after the addition of key raw materials

[0078] Reagent R1:

[0079] Potassium hydrogen phthalate buffer 100mmol / L

[0080] Dodecyltrimethylammonium bromide 40mmol / L

[0081] Alkyl glucoside 6ml / L

[0082] Triton-405 5ml / L

[0083] PC-300 1ml / L

[0084] Sodium sulfate 10g / L

[0085] Reagent R2:

[0086] Phosphate buffer 10mmol / L

[0087] Sodium perbenzoate 8mmol / L

[0088] Sodium persulfate 5mmol / L

[0089] Disodium edetate 40g / L

[0090] PC-300 1ml / L

[0091] Triton-405 5ml / L.

[0092] 2) The method of using the reagents in this example:

[0093] The HCY detection reagent described in this embodiment adopts a full-automatic biochemical analyzer with dual reagent functions during use, such as Hitachi 7180 full-automatic analyzer, etc., and uses the two-point endpoint method to measure; R1 and R2 are determined according to the ratio of 4:1 Place the rat...

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Abstract

The invention relates to a novel reagent for detecting blood total bilirubin through a synthetic chemistry oxidation method. The novel reagent is characterized by comprising a reagent R1 and a reagent R2, the reagent R1 is mainly composed of buffering liquid, accelerator, surfactant, preservative and ion balancer, and the reagent R2 is mainly composed of buffering liquid, sodium perbenzoate, sodium persulfate, masking agent, preservative and surfactant. Sodium perbenzoate is adopted preferentially as a main oxidant, sodium persulfate is used as an auxiliary oxidant, and proper accelerator and surfactant are selected properly, so that the novel reagent has the advantages of high accuracy, high interference resistance and wide linearity range. The novel reagent is quite low in cost and highly conducive to popularization and application on market.

Description

technical field [0001] The invention relates to a novel comprehensive chemical oxidation method for detecting blood total bilirubin detection reagent. Background technique [0002] Most of the bilirubin in the serum is derived from the hemoglobin produced after the destruction of aging red blood cells. The glucuronidated bilirubin in the liver is called direct bilirubin, and the glucuronidated bilirubin in the liver is called indirect bilirubin. The sum of the two is total bilirubin; total bilirubin is widely used in clinical practice, and it is mainly used to diagnose liver disease and biliary tract obstruction in clinical practice. When serum total bilirubin is greatly increased, human The skin, eye sclera, urine and serum are yellow, so it is called jaundice; when the liver is damaged by inflammation, necrosis, poisoning, etc., jaundice can be caused, and biliary tract diseases and hemolytic diseases can also cause jaundice; direct bilirubin increase It is mainly seen in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72
CPCG01N33/728
Inventor 甘宜梧谭柏清罗维晓李静陆盼
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD