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Antiheparin serum total bilirubin (vanadate oxidation method) detection kit

A detection kit and a technology for total bilirubin, applied in the field of serum total bilirubin detection reagents, can solve problems such as inaccurate detection results, troubles in popularization and use, and abnormal detection results, so as to improve clarity and accuracy degree, the effect of improving specificity

Active Publication Date: 2017-02-15
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Later, with the emergence of the vanadate method for detecting bilirubin in blood and its gradual promotion in China, there are many clinical evaluations. Its owner has good specificity and stability, and has been well received by clinical users. However, with the gradual promotion of clinical use, it was found that when using the vanadate method to detect total bilirubin in the plasma samples obtained by using heparin in the clinic, the detection results were inaccurate, the response curve was abnormal and the detection results were abnormal. , it is easy to lead to low or even negative test results, which causes great troubles in clinical promotion and use

Method used

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  • Antiheparin serum total bilirubin (vanadate oxidation method) detection kit
  • Antiheparin serum total bilirubin (vanadate oxidation method) detection kit
  • Antiheparin serum total bilirubin (vanadate oxidation method) detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Disodium hydrogen phosphate-benzoic acid buffer (pH2.9) 0.1mol / L

[0037] Fatty alcohol polyoxyethylene ether 5g / L-10 g / L

[0038] Alkylphenol polyoxyethylene ether (OP) 4 g / L -8g / L

[0039] Polyethylene glycol 8000 1%-5.0%

[0040] Triton-305 2.5 g / L - 5g / L

[0041] Sodium fluoride 1.0g / L-2.0 g / L

[0042] Tetrasodium EDTA 1g / L-2 g / L

[0043] Reagent R2:

[0044] Phosphate buffer (pH7.0) 10mmol / L

[0045] Disodium EDTA 5g / L-10g / L

[0046] Tetrasodium EDTA 1g / L-2g / L

[0047] Sodium metavanadate 4mmol / L

[0048] The usage method of this embodiment reagent:

[0049] The serum total bilirubin (vanadate oxidation method) detection reagent described in this embodiment adopts a fully automatic biochemical analyzer during use, such as Hitachi 7180 fully automatic analyzer, etc., and utilizes a two-point endpoint method for determination. Set the ratio of sample and reagent to 9:240:60, the detection wavelength is 450nm, place distilled water, standard and sample in th...

Embodiment 2

[0051] Interference test: Take fresh mixed serum, divide it into 2 equal parts, then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches Figure 4 requirements. Then respectively use the reagent obtained in Example 1 to compare and measure the content of serum total cholesterol with the common and recognized serum total cholesterol reagent in the market simultaneously. The measurement results of the control group and the measurement results of each group after adding different interfering substances are shown in Figure 4 . Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0052] Depend on Figure 4 It can be seen that the conditions of ascorbic acid ≤ 20mmol / L, heparin ≤ 0.5%, hemoglobin ≤ 200mg / L, and triglyceride ≤ 22.6mmol / L did not significantly interfere with the test results of the ...

Embodiment 3

[0054] Correlation experiment: use the formula of Example 1 to prepare reagents, and conduct a control test with the serum total bilirubin (vanadate oxidation method) kit of a company approved by the State Food and Drug Administration, which is common in the market, and detect 20 samples simultaneously. Clinical serum samples, test results such as Figure 5 shown. And obtained the correlation curve of the two reagents (such as figure 1 As shown), the test results show that the correlation coefficient of the two kits is 0.999, indicating that there is a great correlation between the two.

[0055] The calibrators and quality controls used in the test were:

[0056] Calibrator: RANDOX926 serum total bilirubin content is 32.2 μmol / L.

[0057] Quality control: RANDOX1005 serum total cholesterol target value is 29.3 μmol / L, target value range: 23.2~35.4 μmol / L.

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Abstract

The invention belongs to the technical field of serum total bilirubin (vanadate oxidation method) detection, and especially relates to a serum total bilirubin detection reagent. The serum total bilirubin detection reagent comprises a reagent R1 and a reagent R2; the reagent R1 contains a disodium hydrogen phosphate-citric acid buffer solution, hexadecyl trimethyl ammonium bromide, fatty alcohol-polyoxyethylene ether, alkylphenol ethoxylates (OP), polyethylene glycol 8000, and lauryl sodium sulfate; and the reagent R2 contains a phosphate buffer solution, ethylenediamine tetraacetic acid disodium salt, and sodium metavanadate. The serum total bilirubin detection reagent is suitable to be used together with a plurality of fully automatic biochemical analyzers, and detection results are not influenced by heparin.

Description

technical field [0001] The invention relates to the technical field of serum total bilirubin (vanadate oxidation method) detection, in particular to a serum total bilirubin (vanadate oxidation method) detection reagent suitable for an automatic biochemical analyzer. Background technique [0002] Total bilirubin is Direct bilirubin and the sum of indirect bilirubin. The main sources of total bilirubin are: ①80%~85% of bilirubin comes from the disintegration of aging red blood cells; Formed; ③ A small amount comes from the destruction and decomposition of hemoproteins, such as myoglobin, peroxidase, and cytochrome. [0003] Total bilirubin is mainly used to diagnose whether there is liver disease or whether there is abnormality in the biliary tract. Between / L is mild jaundice; 170-340μmol / L is moderate jaundice; greater than 340μmol / L is severe jaundice. [0004] The commonly used clinical methods for detecting serum total bilirubin include the diazo method, the bilirubin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/72
CPCG01N21/31G01N33/728
Inventor 甘宜梧胡晓飞罗维晓
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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