New polysaccharide-ester biological material
A technology of polysaccharide esters and new materials, applied in the biological field, can solve problems such as reproductive obstacles in breeding pigs, unremarkable polysaccharide effects, and unstable effects
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Embodiment approach 1
[0017] Strain preparation: Cultivate and purify Taikang Trichoderma harzianum strains on the following medium: 200g of potatoes, 20g of glucose, 18-20g of agar, 1000mL of water, natural pH, sterilized at 121°C for 20min, inoculated, at 28°C After culturing for 6 days, a large number of green conidia were produced.
[0018] Liquid culture: Prepare a combination with 25g of soluble starch, 5g of calcium carbonate, 2g of potassium dihydrogen phosphate, and 5g of ammonium sulfate, add 4.0mL of corn oil, the pH is natural, add 1000mL of water to make a liquid medium, and sterilize at 121°C for 20min. Use an inoculation loop to transfer the conidia of Trichoderma to a test tube containing sterile water, stir to disperse the conidia from the mycelium, filter the mycelium with sterile gauze, and adjust the concentration of the spores to 10 7 cfu / mL, according to the inoculum size of 1%, inoculated into the liquid fermentation medium, cultivated at 28°C and 150r / min for 48 hours, at th...
Embodiment approach 2
[0020] Select 20 pigs with virus symptoms. Divided into a treatment group and a control group, the age, clinical manifestations and disease severity of pigs in the two groups were basically the same. There were 10 cases in the treatment group and 10 cases in the control group. In the treatment group, 5ml of polysaccharide ester was added to 500g of feed and mixed evenly. The control group was kept in isolation and managed routinely. After the treatment, the mental state, body temperature and feed intake of the pigs were observed every day. After 5 days of medication, the clinical symptoms disappeared, and those with normal body temperature, appetite, and mental state were judged cured; after 5 days of medication, the sick pigs’ appetite and mental state improved, and those with normal body temperature were judged as improved; both improved and cured were judged effective; After 10 days, still lack of energy, anorexia or death will be judged as invalid.
[0021] Table 1 The ...
Embodiment approach 3
[0025] Healthy female mice were divided into 3 groups, 10 mice / group, each with an average weight of 20 g. Take well-growing mouse transplanted liver cancer H22 cells and adjust to 2×10 with normal saline 6 The cell suspension at a concentration of 1 / ml was inoculated subcutaneously in the right axillary of mice at a volume of 0.2 ml / mouse. The negative control group and the test group were given N.S. simultaneously. The test group was divided into two subgroups: high-dose polysaccharide ester (70mg / Kg) and low-dose polysaccharide ester (35mg / Kg). Intragastric administration (ig) of 0.3ml / 10g was administered continuously for 10 days, and the tumor was weighed the next day after the administration and the tumor inhibition rate was calculated.
[0026] Table 2 Effects of polysaccharide esters on the growth of mouse liver cancer H22
[0027]
[0028] Note: intragastric administration; the average tumor weight of mice in each group is measurement data.
[0029] Gavage admi...
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