Preparation method for iron-dextrin

A technology for iron dextran and dextran, which is applied in the field of fine chemicals, can solve the problems of difficult control of the hydrolysis process of macromolecular dextran, complicated synthesis and purification processes of iron dextran, environmental pollution and the like, achieves controllable hydrolysis process, improved complexation stability, Environmentally friendly effect

Inactive Publication Date: 2017-03-29
RINGPU TIANJIN BIOLOGICAL PHARMA
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems in the existing preparation process of iron dextran that the hydrolysis process of macromolecular dextran is difficult to control, there are many by-products, and the weight average molecular weight distribution range of the generated low molecular weight dextran is wide, the synthesis and purification process of iron dextran is complicated, the cost is high, and it is easy to pollute the environment etc., the present invention provides a method for synthesizing high-quality iron dextran with short reaction time, simple process, low environmental pollution, narrow product molecular weight distribution range and high iron content

Method used

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  • Preparation method for iron-dextrin
  • Preparation method for iron-dextrin

Examples

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Effect test

Embodiment 1

[0024] Obtaining low molecular weight dextran: Weigh 300g of macromolecular dextran (weight average molecular weight 33208, distribution coefficient 1.56), add 1L of distilled water, heat up to 55°C, stir until dissolved; add Chaetomium according to 30U / g substrate ratio α-1,6-dextranase from genus origin, start timing. After the reaction was carried out for 20 minutes, the temperature began to rise, and the temperature was kept at 100° C. for 10 minutes to inactivate the enzyme activity and terminate the reaction. According to the detection method for the molecular weight of dextran in the 2010 edition of Chinese Veterinary Pharmacopoeia, the weight average molecular weight of dextran was detected to be 5160, and the distribution coefficient was 1.22, which met the standard and could be directly used in the next reaction.

[0025] Synthesis of iron dextran: Dilute the above reaction solution to 3L, heat up to 90°C, add 600ml of 20% sodium hydroxide solution dropwise, cool dow...

Embodiment 2

[0028] Obtaining of low molecular weight dextran: Weigh 500 g of macromolecular dextran (weight average molecular weight 1982410, distribution coefficient 1.52), and add 1 L of distilled water. Raise the temperature to 70°C, stir until dissolved; add α-1,6-dextranase from Penicillium at a ratio of 100 U / g substrate, and start timing. After the reaction was carried out for 90 minutes, the temperature began to rise, and the temperature was maintained at 100° C. for 10 minutes to inactivate the enzyme activity and terminate the reaction. According to the detection method for the molecular weight of dextran in the 2010 edition of Chinese Veterinary Pharmacopoeia, the weight average molecular weight of dextran was detected to be 5580, and the distribution coefficient was 1.35, which met the standard and could be used for the next reaction.

[0029] Synthesis of iron dextran: Take the above reaction solution, heat up to 65°C, add 13ml of dimethyl sulfoxide, 40ml of 30% hydrogen pero...

Embodiment 3

[0032]Obtaining low molecular weight dextran: Weigh 100g of macromolecular dextran (weight average molecular weight 7156, distribution coefficient 1.14), add it to 2L distilled water, raise the temperature to 35°C, stir until dissolved; add hair shell according to the ratio of 5U / g substrate α-1,6-dextranase derived from Bacteria, start the timer. After the reaction was carried out for 15 minutes, the temperature began to rise, and the temperature was kept at 100° C. for 10 minutes to inactivate the enzyme activity and terminate the reaction. According to the detection method for the molecular weight of dextran in the 2010 edition of Chinese Veterinary Pharmacopoeia, the weight-average molecular weight of dextran was detected to be 5233, and the distribution coefficient was 1.17, which met the standard and could be directly used in the next reaction.

[0033] Synthesis of iron dextran: take the above reaction solution, heat up to 90°C, then add 50ml of 20% sodium hydroxide sol...

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Abstract

The invention discloses a preparation method for iron-dextrin. The preparation method comprises the following steps: with macro-molecular dextran as a substrate, subjecting macro-molecular dextran to hydrolysis with dextranase originated from a specific bacterial strain so as to obtain low-molecular-weight dextran; activating the active and inert hydroxyl groups of the low-molecular-weight dextran by using an alkalization-oxidation combined method; complexing the activated dextran with iron hydroxide so as to obtain an iron-dextrin reaction solution; and successively carrying out centrifugation for impurity removal, ultrafiltration for removal of heavy metals and impurity ions, and freeze drying or spray drying so as to obtain finished iron-dextrin crystal powder. The finished iron-dextrin prepared in the invention has iron content of 40% or above.

Description

technical field [0001] The invention relates to a preparation method of iron dextran, that is, a preparation process of polysaccharides and derivatives thereof, belonging to the field of fine chemicals. Background technique [0002] Iron dextran is a commonly used anti-anemia drug in veterinary medicine, and it is suitable for the prevention and treatment of iron deficiency anemia in large and small livestock. Iron is the main component of hemoglobin and myoglobin. Hemoglobin is the main oxygen carrier of red blood cells. Myoglobin is the place where muscle cells store oxygen to meet the oxygen supply needs of muscles during exercise. Most enzymes involved in the Krebs cycle contain iron, or function only in the presence of iron. Therefore, after actively supplementing iron supplements to iron-deficient livestock, in addition to accelerating the synthesis of hemoglobin, symptoms related to tissue iron deficiency and iron-containing enzyme activity reduction, such as growt...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 魏微李旭东刘爱玲井丁丁刘桂兰
Owner RINGPU TIANJIN BIOLOGICAL PHARMA
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