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Low-cost preparation method of specific vitelline fluid additive utilizing high-adsorbability rice hull powder as carrier and specially used for stichopus japonicus

A technology of high adsorption and rice husk powder, applied in the direction of additional food elements, chemical instruments and methods, molding or processing of animal feed, etc., can solve the problem of high cost, achieve low equipment cost, enhance the body's immunity and disease resistance Remarkable effect of power and energy saving

Inactive Publication Date: 2017-05-10
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the current egg yolk antibody products are egg yolk powder and whole egg powder, but the traditional production process needs to be freeze-dried or spray-dried, and the cost is relatively high

Method used

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  • Low-cost preparation method of specific vitelline fluid additive utilizing high-adsorbability rice hull powder as carrier and specially used for stichopus japonicus
  • Low-cost preparation method of specific vitelline fluid additive utilizing high-adsorbability rice hull powder as carrier and specially used for stichopus japonicus
  • Low-cost preparation method of specific vitelline fluid additive utilizing high-adsorbability rice hull powder as carrier and specially used for stichopus japonicus

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Experimental program
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Effect test

Embodiment 1

[0028] The preparation method of sea cucumber specific anti-Shewanella yellow sea specific egg yolk liquid additive comprises the following steps:

[0029] (1) separating the egg yolk and the egg white of the anti-Shewanella yellowsea high-free egg, getting the egg yolk and pouring it into a container, and stirring;

[0030] (2) Stir the egg yolk until the volume expands, the color becomes lighter, and becomes thick, and the mass is weighed;

[0031] (3) Add the highly absorbing rice husk powder of 3 / 7-3 / 2 of the quality of the egg yolk liquid in small amounts for several times, and stir while adding, so that the highly absorbing rice husk powder and the egg yolk liquid are completely mixed;

[0032] (4) Place the homogeneously mixed egg yolk liquid and rice husk powder mixture in a feed pellet machine to press into pellets with a diameter of 2-10mm, and place them in a cool place indoors to dry naturally until the moisture content is reduced to below 10%;

[0033] (5) Place ...

Embodiment 2

[0035] The preparation method of sea cucumber specific anti-Vibrio splendidus specific egg yolk liquid additive comprises the following steps:

[0036] (1) Separate the egg yolk and the egg white of the anti-vibrio brilliant high-free egg, get the egg yolk and beat it into a container, and stir;

[0037] (2) Stir the egg yolk until the volume expands, the color becomes lighter, and becomes thick, and the mass is weighed;

[0038] (3) Add the highly absorbing rice husk powder of 3 / 7-3 / 2 of the quality of the egg yolk liquid in small amounts for several times, and stir while adding, so that the highly absorbing rice husk powder and the egg yolk liquid are completely mixed;

[0039] (4) Place the homogeneously mixed egg yolk liquid and rice husk powder mixture in a feed pellet machine to press into pellets with a diameter of 2-10mm, and place them in a cool place indoors to dry naturally until the moisture content is reduced to below 10%;

[0040] (5) Place the dried particles i...

Embodiment 3

[0042] Determination of IgY content in Apostichopus japonicus special specific egg yolk liquid additive

[0043] The standard IgY coating solution was diluted to a certain gradient (1-0.015625ug / ml), and the specific additive sample was also diluted to a certain gradient, and 100ul was added to each well to coat a 96-well microtiter plate, overnight at 4°C. Wash the ELISA plate 3 times with washing solution, add 100ul of blocking solution to each well, incubate at 37°C, spin dry, wash 3 times, then add 100ul of rabbit anti-chicken IgG-HRP to each well, react at 37°C for 1h; wash with washing solution 5 times in each well, add 100ul TMB substrate solution to each well, develop color at room temperature for 20min, add 50ul stop solution to each well to stop the reaction, measure OD with a microplate reader 450 / 630 . Make a standard curve with the OD value of standard IgY against the concentration of IgY, and find the concentration of the sample to be tested from the standard cu...

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Abstract

The invention discloses a low-cost preparation method for a specific vitelline fluid additive utilizing high-adsorbability rice hull powder as a carrier and specially used for stichopus japonicus, belonging to the technical field of feeds. The low-cost preparation method comprises the steps of processing 30-60-mesh rice hull powder by virtue of a freezing-expanding-peeling technique, and adding the following components in percentage by mass: 40%-70% of vitelline fluid containing specific egg yolk antibodies, 30%-60% of high-adsorbability rice hull powder and 0.05%-0.1% of potassium sorbate. 1%-5% of the specific vitelline fluid additive is uniformly mixed with a stichopus japonicas compound feed, and the mixture is applied to a seedling pond, so that the stichopus japonicus skin ulcer syndrome or other bacteriosis can be controlled, and the organism immunity of the stichopus japonicas can be enhanced. Besides, a production process of the specific vitelline fluid additive is greatly simplified, the production cost is greatly lowered, and the low-cost preparation method is more suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of feed, and relates to a new specific egg yolk feed additive and a preparation method for preventing and treating sea cucumber rot skin syndrome and other bacterial diseases. Background technique [0002] Sea cucumber (Apostichopus japonicas), also known as imitation sea cucumber, belongs to the phylum Echinodermate, the class Holothrioider, the family Stichopodidae, and the genus Apostichopus. In my country, it is mainly produced in the northern coastal areas such as Liaoning Province and Shandong Province. Because of its high nutritional value and medicinal value, it has become one of the most important and precious breeding species in the northern region. With the continuous expansion of the breeding scale, the disease problem of sea cucumber is becoming more and more serious. At present, the common diseases mainly include rot skin syndrome. Symptoms, swelling around the mouth, rotten edges, rotten stom...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/30A23K10/20A23K40/10A23K20/105A61K39/395A61P17/02A61P31/04
CPCA61K2039/505C07K16/02C07K16/12C07K2317/23Y02P60/87
Inventor 徐永平李纪彬杜正江
Owner DALIAN SEM BIOLOGICAL ENG TECH