Hollow mesoporous silica nanoparticles, nanocarriers and preparation methods thereof

A mesoporous silica and nano-carrier technology, applied in the direction of silica, silicon oxide, nanotechnology, etc., can solve the problems of limiting the prospect of in vivo application, weak transfection ability of antiserum, and decreased transfection efficiency, achieving Under the cytotoxicity of the vector, the effect of increasing the transfection efficiency and high gene transfection efficiency
CN106698447BActive Publication Date: 2020-06-16GUANGZHOU ZHONGDA NANSHA TECH INNOVATION IND PARK +1
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU ZHONGDA NANSHA TECH INNOVATION IND PARK
Publication Date
2020-06-16

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a hollow mesoporous silicon dioxide nanoparticle and a preparation method thereof. The preparation method comprises the following steps: magnetically stirring and mixing ethanol, deionized water and ammonia water at a certain temperature, adding ethyl orthosilicate for continuous reaction, adding premixed ethyl orthosilicate and trimethoxyoctadecylsilane for continuous reaction, vacuum drying an obtained mixture after being etched by sodium carbonate, calcining at 550 DEG C to obtain the hollow mesoporous silicon dioxide nanoparticle. The nanoparticle is dispersed in ultrapure water, and polymine is added to obtain a hollow mesoporous silicon dioxide nano-carrier after mixing; and the hollow mesoporous silicon dioxide nano-carrier is mixed with a gene to obtain a hollow mesoporous silicon dioxide gene nano-carrier. The hollow mesoporous silicon dioxide nano-carrier prepared by the preparation method disclosed by the invention has the advantages of good dispersity, high gene loading capacity and high transfection efficiency (twice of 25kDa PEI), and has a practical value of clinical application.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of pharmaceutical preparations, in particular to a hollow mesoporous silicon dioxide nanoparticle, a nanocarrier and a preparation method thereof. Background technique

[0002] Gene therapy refers to the transfer of exogenous genes with therapeutic effects into specific cells in a certain way to achieve the purpose of treatment. Among them, how to effectively deliver the target gene to cells and exert curative effect is the key to this therapy. Gene delivery usually requires the help of vectors, and the current gene vectors are often divided into two types: viral vectors and non-viral vectors. Although viral vectors can achieve high transfection efficiency, high immunogenicity, possible carcinogenicity and other safety issues limit the wide application of such vectors. Non-viral vectors have attracted the attention of researchers due to their low immunogenicity, high safety, and convenient preparation. This type of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More