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Accelerator for anaerobic fermentation of antibiotic antibacterial residue and preparation and application of accelerator

An antibiotic slag, anaerobic fermentation technology, applied in fermentation, biochemical equipment and methods, bacteria and other directions, can solve the mismatch between processing capacity and production, low organic load of anaerobic fermentation of bacterial slag, gas production efficiency and comprehensive problems such as poor efficiency, to achieve the effect of promoting sustainable development, promoting the growth of methane bacteria, and improving gas production efficiency

Active Publication Date: 2017-05-31
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some scientific research institutes and pharmaceutical companies have carried out anaerobic fermentation treatment of medicinal dregs and achieved initial results, there are many problems, one of the key problems is that antibiotic dregs are mainly mycelium, which is difficult to break through Treatment, anaerobic fermentation of bacterial residues has low organic load, poor gas production efficiency and comprehensive benefits, unstable system operation, serious mismatch between processing capacity and production volume, far from meeting processing needs

Method used

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  • Accelerator for anaerobic fermentation of antibiotic antibacterial residue and preparation and application of accelerator
  • Accelerator for anaerobic fermentation of antibiotic antibacterial residue and preparation and application of accelerator
  • Accelerator for anaerobic fermentation of antibiotic antibacterial residue and preparation and application of accelerator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Preparation of Anaerobic Fermentation Accelerator

[0016] (1) Functional microbial culture

[0017] Both Y10 and Z13 were cultured in LB medium with shaking at 30 °C for 72 h in an anaerobic bottle. It was detected that 80% of the bacteria in the fermentation liquid formed spores, and they were placed at 4 °C for later use. Centrifuge at 8000 rpm / min for 15 min before use, collect the bacteria separately, dilute with a small amount of sterile water, and adjust the concentration of spores to (1.0-5.0)×10 11 cfu / mL.

[0018] LB medium: 5.0 g of yeast extract, 10.0 g of tryptone, 10.0 g of sodium chloride, 15.0 g of agar, 1000 mL of water, pH7.0-7.4.

[0019] (2) Trace element stock solution configuration: FeSO 4 ·7H 2 O, NiCl 2 ·6H 2 O and CoCl 2 ·6H 2 O is prepared to a relatively high concentration, such as a stock solution of 1-10 mol / L for later use.

[0020] (3) Take an appropriate amount of biochar, dry it in an oven, and pulverize it. Mix func...

Embodiment 2

[0021] Example 2 Anaerobic fermentation of cephalosporin C bacteria residue

[0022] A 250 mL anaerobic bottle was used, with cephalosporin C residue as raw material, and a medium-temperature full-mix anaerobic fermentation process. Two treatments were set up: treatment one: blank control; treatment two: adding 1.5% of the present invention accelerators. The organic load TS is 2%, (TS is also called the dry matter concentration, which refers to placing a certain amount of fermentation feed liquid in an oven at 100~105 °C and drying it to a constant weight, and the percentage of the dried material to the total weight). The biogas slurry in the fermentation biogas digester was used as the inoculum, the inoculum amount was 20-30% (volume ratio), the fermentation temperature was 35±2°C, the fermentation time was 30 days, and 5 parallels were used for each treatment. The specific results are shown in Table 1.

[0023] Table 1 Biogas production situation

[0024]

[0025] As c...

Embodiment 3

[0026] Embodiment 3 Batch type anaerobic fermentation experiment

[0027] A 250 mL anaerobic bottle was used, with penicillin residue as raw material, and a medium-temperature full-mix anaerobic fermentation process. Seven treatments were set up: treatment 1: blank control; treatment 2: adding biochar with a mass fraction of 1%; treatment 3: Add Bacillus amyloliquefaciens Y10 1.0 x 10 7 cfu / mL; treatment four: add bienzyme Clostridium Z-13 1.0×10 7 cfu / mL; treatment five: add mixed functional microorganisms, in which Bacillus amyloliquefaciens Y10 and Clostridium bienzyme Z-13 are mixed at a ratio of 1:1, and the dosage is 1.0×10 7 cfu / mL; treatment six: mixed trace elements, of which Fe 2+ 300 mg / L, Ni 2+ 0.5 mg / L, Co 2+ 50 mg / L. Treatment seven: Add the accelerator prepared by the present invention with a mass fraction of 1% (after adding, the fermentation system will contain Bacillus amyloliquefaciens Y100.5×10 7 cfu / mL, Bienzyme Clostridium Z-130.5×10 7 cfu / ...

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Abstract

The invention discloses an accelerator for anaerobic fermentation of an antibiotic bacterial residue. The accelerator comprises functional microbes, a microelement and a carrier, wherein the functional microbes are bacillus amyloliquefaciens Y10 and clostridium bifermentans Z-13;charcoal is used as a carrier, the microelement comprises Fe<2+>, Co<2+> and Ni<2+>. The preparation method comprises the steps of mixing various components evenly, drying and then crushing or granulating. The accelerator is used for anaerobic fermentation of a high-protein waste to produce a biogas, especially anaerobic fermentation of the antibiotic antibacterial residue to produce the biogas; organic loads of the antibiotic antibacterial residue can be improved to 4.5-5.5kg / m<3>d from 2.5kg / m<3>d; and the yield of the biogas is improved by more than or equal to 40%.

Description

technical field [0001] The invention relates to the technical field of high-efficiency resource treatment of antibiotic residues, in particular to an anaerobic fermentation accelerator for antibiotic residues and its preparation and application. Background technique [0002] my country is the world's largest producer and exporter of antibiotic raw materials. The production of 1 ton of antibiotics produces about 8 to 10 tons of wet fungal residues. Therefore, the annual discharge of antibiotic residues reaches millions of tons. The residual culture medium and antibiotics in antibiotic residues The parent body and its degradation products are potentially harmful to the ecological environment and human health. The country has clearly listed antibiotic dregs as hazardous waste and prohibited them from being used in feed and fertilizer. The disposal method approved by the current policy is mainly incineration, and the incineration of 1 ton of medicinal residues costs about 1,500 t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C12P5/02C12R1/07C12R1/145
CPCC12N1/20C12N11/14C12P5/023Y02E50/30
Inventor 程辉彩何强张丽萍习彦花崔冠慧史延茂赵燕肖张飞燕杨继业刘倩倩刘洪伟尹淑丽
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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