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Water-soluble microbial agent as well as preparation method and application thereof

A microbial inoculum and water-soluble technology, applied in the field of water-soluble microbial inoculants, can solve the problems of excessive fertilizer and pesticide residues, obstacles to continuous cropping, etc., and achieve the effects of inhibiting the breeding of miscellaneous bacteria, promoting decomposition, and improving the micro-ecological environment.

Inactive Publication Date: 2017-05-31
北京平安福生物技术研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first technical problem to be solved by the present invention is to provide a water-soluble microbial bacterial agent, which can significantly improve the problems of excessive fertilizer and pesticide residues in the soil of facility greenhouses and serious obstacles to repeated cropping, and can improve the soil ecological environment. , and the effect of improving crop yield or quality is remarkable;

Method used

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  • Water-soluble microbial agent as well as preparation method and application thereof
  • Water-soluble microbial agent as well as preparation method and application thereof
  • Water-soluble microbial agent as well as preparation method and application thereof

Examples

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preparation example Construction

[0027] Preparation of Preliminary Example 1 Bacillus subtilis bacterial agent

[0028] The bacillus subtilis solid microbial agent can be prepared according to conventional methods, for example, the bacillus subtilis bacterial strain that buys is inoculated on the seed culture and cultivated to obtain the seed culture; the seed culture is inoculated into the fermentation medium in the fermenter to ferment After the fermentation is completed, fillers and pulverized adsorbents are added to obtain the solid bacterial agent of Bacillus subtilis; specifically, it can be prepared by referring to the following method:

[0029] (1) After inoculating Bacillus subtilis (Bacillus subtilis) (strain number AS 1.11086) on a plate and culturing twice, culture it in a 250 ml eggplant bottle with nutrient agar medium at a culture temperature of 37°C for 36 hours When the growth is good, wash it with sterilized water and use it as the inoculum of the seed tank;

[0030] (2) Put in the medium a...

Embodiment 2

[0032] Preparation of Preliminary Example 2 Lactobacillus plantarum inoculum

[0033] Inoculate Lactobacillus plantarum strains in beef extract-peptone slant medium for culture (3 grams of beef extract, 5-10 grams of peptone, 5 grams of NaCl, dissolve the above ingredients in distilled water and adjust the volume to 1000 ml, adjust the pH value to 7), Add mineral oil to the slant seed strain of beef juice peptone medium and store it in a refrigerator at 4°C. Take it out and activate it twice before use, and transfer the activated strain into a 250 ml eggplant bottle for cultivation at a temperature of 28°C for 48 hours. When the strain grows well, it is washed with sterilized physiological saline, and the eluate is the inoculum of the seed tank. Add culture medium to a 1 ton fermenter, and its composition is: corn flour 20kg, bean cake powder 20kg, K 2 HPO 4 7.2kg, KH 2 PO 4 5.3kg, MgSO 4 0.5kg, peanut oil 2kg, water 45kg, mix the above components evenly, adjust the pH...

Embodiment 1

[0041] The preparation of embodiment 1 water-soluble microbial bacterial agent

[0042] Weigh each component according to the following weight (unit: kg):

[0043] Fulvic acid (purchased from Shanghai Tong Microorganism Technology Co., Ltd., trade name "fulvic acid") 60, amino acid powder (purchased from Sichuan Mianzhu Xinhua Chemical Co., Ltd., trade name: "fertilizer grade amino acid powder") 30, acetic acid 5, Molasses 5, Lactobacillus plantarum 1, Bacillus lateralis 1, Bacillus subtilis 1, Bacillus licheniformis 1, Bacillus amyloliquefaciens 1, water 96.

[0044] Preparation method: Mix all components evenly, and anaerobically ferment at 30°C for 70 hours to obtain the product.

[0045] The preparation of embodiment 2 water-soluble microbial bacterial agents

[0046] Weigh each component according to the following weight (unit: kg):

[0047] Fulvic acid 60, amino acid 40, acetic acid 10, molasses 10, Lactobacillus plantarum 1, Bacillus lateralis 1, Bacillus subtilis 1,...

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Abstract

The invention discloses a water-soluble microbial agent as well as a preparation method and application thereof. The invention first discloses a water-soluble microbial agent which comprises a bacillus subtilis agent, a bacillus licheniformis agent, a bacillus amyloliquefaciens agent, fulvic acid, amino acid, acetic acid and molasses. The water-soluble microbial agent is further added with a lactobacillus plantarum agent and a bacillus laterosporus agent to realize the effects of better improving the micro-ecological environment of soil and improving the crop yield and quality. The invention also discloses a method for preparing the water-soluble microbial agent, which comprises the following steps: weighing the components according to the weight parts; adding water and mixing uniformly; and performing anaerobic fermentation to obtain the water-soluble microbial agent. In the water-soluble microbial agent disclosed by the invention, the agents therein are reasonably matched to realize an obvious synergistic effect, the soil structure can be effectively regulated, the continuous cropping obstacle is relieved, the water-fertilizer retaining ability of soil is enhanced, and the crop yield and quality are improved.

Description

technical field [0001] The invention relates to a water-soluble microbial bacterial agent, and also relates to a preparation method of the water-soluble microbial bacterial agent and its application in facility greenhouse soil, belonging to the field of preparation and application of microbial bacterial agents. Background technique [0002] Due to the excessive application of chemical fertilizers and pesticides for many years in facility greenhouses, the soil is compacted and degraded, and repeated cropping obstacles often occur, resulting in frequent occurrence of crop diseases and insect pests. The microbial agent is irrigated into the soil with water, and through the proliferation of beneficial microorganisms, it can promote the transformation of nutrients in organic materials, supplement and balance the effective nutrients in the soil, promote the decomposition of organic matter, transform into high-quality organic fertilizers, reduce soil bulk density, and promote soil g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C05G3/04A01B79/02C12R1/125C12R1/10C12R1/07C05G3/80
CPCA01B79/02C05C11/00C05G3/00C05G3/80C12N1/20C05F5/00C05F11/08
Inventor 殷汝新岳寿松
Owner 北京平安福生物技术研究所有限公司
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