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Aptamer sensor for detecting bisphenol A and preparation method of aptamer sensor

An aptamer sensor and aptamer technology, applied in the field of electrochemical biosensors, can solve the problems of not being reached, and achieve the effects of low detection limit, good recovery rate, and strong specificity

Inactive Publication Date: 2017-06-13
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

but not yet below 10 -12 Related methods and reports of mol / L

Method used

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  • Aptamer sensor for detecting bisphenol A and preparation method of aptamer sensor
  • Aptamer sensor for detecting bisphenol A and preparation method of aptamer sensor
  • Aptamer sensor for detecting bisphenol A and preparation method of aptamer sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Preparation of an aptasensor for detecting bisphenol A (1)

[0029] Conductive diamond films (boron doped) were deposited on silicon substrates by microwave plasma chemical vapor deposition equipment. Ion sputtering gold target 6 seconds on diamond surface then, obtain gold film / boron-doped diamond film structure (see figure 2 ), the sample was transferred to a high-temperature tube furnace, annealed at a high temperature for 1 minute, and washed with deionized water to obtain a gold nanoparticle / diamond film structure (see image 3 ). Add 2 μmol / L aptamer dropwise to the surface of gold nanoparticles / boron-doped diamond film, seal it overnight at 37-39 °C, and rinse it with buffer solution; then immerse the obtained electrode in 1 mmol / L mercaptohexanol (6 -Mercaptohexan-1-ol (MCH)) solution was modified for 2 hours to obtain an aptamer sensor for detecting bisphenol A.

[0030]The aptamer is an oligonucleotide sequence or a short polypeptide screened fr...

Embodiment 2

[0032] Example 2: Preparation of an aptasensor for detecting bisphenol A (2)

[0033] The boron-doped conductive diamond film is deposited on the silicon substrate by microwave plasma chemical vapor deposition or / and hot wire chemical vapor deposition. Then sputtering a layer of gold film on the surface of the conductive diamond by means of electron sputtering or magnetron sputtering for 5-8 seconds and with a thickness of 10-30nm. The resulting gold film / diamond film is annealed at 800-850° C. for 1-2 minutes in a high-temperature tube furnace, and cleaned with deionized water to obtain a gold nanoparticle / diamond film structure (see image 3 ). In an airtight container, disperse 2-2.5 μmol / L of mercapto-modified aptamers on the surface of dry gold nanoparticles / diamonds, assemble overnight at 37-39 ° C, soak and wash in deionized water for 2-3 hours, and obtain Bisphenol A aptasensor.

[0034] The thiol-modified aptamer in this example can be selected from the product syn...

Embodiment 3

[0035] Example 3: Preparation of an aptasensor for detecting bisphenol A (3)

[0036] On the basis of Example 1, the tube furnace sintering and high-temperature annealing can be performed for 10-30 seconds; the modification in 6-mercaptohexan-1-ol (MCH) solution can also be soaked for 0.5-1 hour.

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Abstract

The invention provides an aptamer sensor for detecting bisphenol A and a preparation method of the aptamer sensor and belongs to the technical field of electrochemical biosensors. The bisphenol A aptamer sensor is characterized in that gold nanoparticles (2) are deposited on a boron-doped diamond film (1); an aptamer (2) is self-assembled on the gold nanoparticles; mercaptoethanol (4) occupies a blank site left by the aptamer on the gold nanoparticles. A gold film is deposited on the boron-doped diamond film and is annealed in a tubular furnace to obtain the gold nanoparticles; the preparation of the bisphenol A aptamer sensor is finished by utilizing self-assembling of the aptamer and the mercaptoethanol. According to the aptamer sensor provided by the invention, the detection capability of the bisphenol A is easy to improve by selecting boron-doped diamond and taking a reasonable structure design; the novel bisphenol A aptamer sensor has the advantages of high sensitivity, high specificity, good repeatability, low detection limit, rapidness in detection, high efficiency, high stability, simple preparation process and low cost.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors, and relates to a composite sensor made of conductive diamond film, gold nanoparticles, aptamer and mercaptohexanol and a preparation method thereof. Background technique [0002] The existence of organic pollutants such as pesticides and medicines seriously affects the aquatic ecosystem and human health. Over the past few decades, endocrine disruptors such as bisphenol A have attracted widespread attention around the world. Bisphenol A is widely used in the chemical industry, mainly for the manufacture of plastics, such as food packaging, beverage bottles, storage equipment, tableware, feeding bottles, medical equipment, etc. However, the toxicity of BPA can lead to human developmental disorders, miscarriage, coronary heart disease and breast cancer, among others. Many countries have therefore banned BPA in baby bottles and other packaging. The current daily intake announced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30C23C16/27C23C14/35C23C14/34C23C14/18C23C14/58C23C28/00
CPCG01N27/308C23C14/185C23C14/3478C23C14/35C23C14/5806C23C16/271C23C16/274C23C16/276C23C28/322C23C28/34
Inventor 李红东马一博刘钧松王启亮成绍恒
Owner JILIN UNIV
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