Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of lipopeptide biosurfactant

A biological surface and active agent technology, applied in the biological field, can solve the problems of hindering industrialization, low output, high production cost, etc., and achieve the effect of reducing operation steps, reducing production cost and saving pretreatment cost

Active Publication Date: 2017-06-16
NANJING UNIV OF TECH
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the low yield of lipopeptide biosurfactants at the present stage, the high production cost hinders its industrialization and other defects, the purpose of the present invention is to provide a preparation method of lipopeptide biosurfactants with high efficiency utilization of xylose

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of lipopeptide biosurfactant
  • Preparation method of lipopeptide biosurfactant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example demonstrates that Bacillus subtilis Bacillus subtilis G-34 utilizes xylose to produce lipopeptide biosurfactants.

[0027] (1) Preparation of seed medium: yeast extract powder 5g / L, peptone 10g / L, sodium chloride 10g / L; sterilize at 121°C for 20min, cool down for later use.

[0028] (2) Fermentation medium preparation: xylose 20-30g / L, magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.02g / L, potassium dihydrogen phosphate 10g / L, nitrogen source: feather hydrolysis Waste liquid, MSG fermentation waste liquid, or yeast extract powder (N content of nitrogen source is 50-100mmol / L), use NaOH solution (6mol / L) to adjust the pH to 7.5. Sterilize at 115°C for 20min. Let cool and set aside.

[0029] Feather hydrolysis waste liquid is provided by Jiangsu New Hanling Bioengineering Co., Ltd. (Jiangsu, Xinyi). The ingredients contain 18-20% of total amino acids, NH 4 Cl 20-25%, small peptide and ash 2%.

[0030] The monosodium glutamate ferme...

Embodiment 2

[0038] This example illustrates and common Ca(OH) 2Compared with neutralization and detoxification, NaOH neutralization is a better method for pretreatment of corncob / corn stalk acid hydrolysis solution.

[0039] (1) The seed medium is the same as that in Example 1, and the cultivation method adopts the method adopted in Example 1 for cultivation.

[0040] (2) Fermentation medium: corn cob acid hydrolysis solution or corn stalk acid hydrolysis solution (containing 20g / L of total sugar), feather hydrolysis waste liquid (containing N content of 50mmol / L), magnesium sulfate heptahydrate 0.2g / L, Ferrous sulfate heptahydrate 0.02g / L, potassium dihydrogen phosphate 10g / L, use NaOH solution (6mol / L) to adjust the pH to 8.0. Sterilize at 115°C for 20min. Let cool and set aside.

[0041] The preparation method of corn cob / corn stalk acid hydrolysis solution is: crush corn cob or corn stalk into granules, mix corn cob or corn stalk granules with dilute sulfuric acid with a concentrati...

Embodiment 3

[0047] This example shows that Bacillus subtilis G-34 has strong tolerance to various inhibitory components in corncob acid hydrolysis solution.

[0048] (1) The seed medium is the same as that in Example 1, and the cultivation method adopts the method adopted in Example 1 for cultivation.

[0049] (2) Fermentation medium: corn cob acid hydrolysis solution (total sugar 30g / L), magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.02g / L, potassium dihydrogen phosphate 10g / L, nitrogen source is Feather hydrolysis waste liquid (N content of nitrogen source is 50mmol / L), using NaOH solution (6mol / L) to adjust the pH to 7.8. Sterilize at 115°C for 20min. Let cool and set aside. Formic acid (65mmol / L), acetic acid (45mmol / L), furfural (12mmol / L), 5-hydroxymethylfurfural (12mmol / L) and other biomass produced in the dilute acid hydrolysis process were added to experimental groups 1-4. Inhibit ingredients.

[0050] The sample in Example 1 was used as a control group....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a lipopeptide biosurfactant. Bacillus subtilis G-34 is taken as a fermentation strain, and xylose is taken as a main carbon source to be fermented to prepare the biosurfactant; the registration collection number of the strain is CCTCC NO: M2014003, wherein the carbon source is xylose, a xylose mother liquor or an acidolysis solution of agricultural by-products; a fermented nitrogen source selects cheap biomass waste such as feather hydrolysis waste liquid and monosodium glutamate fermentation waste liquid; acetic acid is added to a fermentation medium to promote the strain fermentation so as to prepare the biosurfactant. The method disclosed by the invention efficiently utilizes cheap agricultural waste as a raw material to produce the lipopeptide biosurfactant so as to change the agricultural waste into valuables, so that the production cost of the lipopeptide biosurfactant is greatly lowered to provide technical support for industrial production of the lipopeptide biosurfactant.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of a lipopeptide biosurfactant. Background technique [0002] Lipopeptide biosurfactants are secondary metabolites of Bacillus subtilis, originally discovered by Arima in the fermentation broth of Bacillus subtilis. The lipopeptide biosurfactant molecule is mainly composed of two parts, one part is a hydrophobic fatty acid chain, and the other part is a hydrophilic peptide ring. This molecular structure makes lipopeptide biosurfactants have good surface activity. According to the literature It has been reported that lipopeptide biosurfactants are one of the best surfactants reported so far. As early as the 1940s, Zobell pointed out that the surfactant produced by microorganisms is one of the main mechanisms of microbial flooding. With the increasing shortage of oil resources and the strengthening of people's awareness of environmental protection, pe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/00C12R1/125
CPCC12P21/00
Inventor 李霜陈晨易高斌宋萍
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com