Preparation method of silk fibroin/graphene composite nano fiber support material

A technology of graphene composite and silk fibroin, which is applied in the field of preparation of silk fibroin/graphene composite nanofiber scaffold materials, can solve the problems of low conductivity, small dosage, weak electrochemical signal, etc., and achieve a simple and easy preparation method The effect of high efficiency, abundant raw material resources, and good biocompatibility

Inactive Publication Date: 2017-07-04
WUHAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many studies have modified graphene or graphene oxide on silk fibroin, such as patent application number CN201610806923.5: preparation method based on graphene oxide composite nanofiber tissue engineering scaffold, although the addition of graphene oxide can make the composite membrane The mechanical properties have been improved, but the conductivity of this composite scaffold material is low, the electrochemical signal between neurons is weak, and it cannot promote cell proliferation well; patent application number CN201510106514.X: a silk fibroin / graphite The preparation method of ene conductive fiber uses partially reduced graphene oxide to modify silk fibroin, although it can effectively solve the problem of easy agglomeration of graphene and effectively improve the performance of silk fibroin, but it is different from the performance of graphene There is still a certain gap in comparison, mainly because the dosage is small and the dispersion is poor

Method used

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  • Preparation method of silk fibroin/graphene composite nano fiber support material
  • Preparation method of silk fibroin/graphene composite nano fiber support material
  • Preparation method of silk fibroin/graphene composite nano fiber support material

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Embodiment 1

[0033] The present embodiment provides a kind of preparation method of silk fibroin / graphene composite nanofiber support material, described method comprises the following steps:

[0034] First add 30g silkworm cocoons to 1L 0.5% Nɑ 2 CO 3 In an aqueous solution, boil for 40 minutes and keep the temperature at a constant temperature for 2 hours, repeat the treatment three times, fully wash with deionized water, and place it in a drying oven at 50° C. to dry at a constant temperature after washing to obtain degummed silk. Add the dried silk to a 70°C ternary mixed solution of calcium chloride, absolute ethanol and water with a molar ratio of 1:8:2, dissolve it into a silk fibroin solution, and dialyze it with deionized water for 72 hours to remove the Ethanol and calcium chloride were used to prepare an aqueous silk fibroin solution, which was then freeze-dried at -80°C to obtain a pure silk fibroin sample.

[0035] Dissolve 0.32g silk fibroin in 4mL hexafluoroisopropanol, st...

Embodiment 2

[0039] Weigh 0.32 g of silk fibroin prepared in Example 1 and dissolve it in 4 mL of hexafluoroisopropanol, stir until completely dissolved to obtain 0.08 g / mL silk fibroin liquid A, and take 1.6 mL of graphene with a pipette gun Centrifuge the ethanol dispersion at high speed, suck out the ethanol, transfer the graphene in the centrifuge tube to the silk fibroin solution with hexafluoroisopropanol, add a certain amount of hexafluoroisopropanol to make the silk fibroin solution reach 6mL, and ultrasonicate for 60min. Finally, the 0.06g / mL silk fibroin spinning solution B whose mass ratio of graphene and silk fibroin is 1:200 is obtained, and the spinning solution is injected into a 10mL syringe for electrospinning, and the process conditions are: spinning voltage 16kv, pushing speed 0.05mm / min, receiving distance 12cm, roller speed 100r / min. The obtained electrospun membrane was treated with absolute ethanol for 4 hours, and then dried in a vacuum oven at 50° C. for 24 hours t...

Embodiment 3

[0042]Weigh 0.32 g of silk fibroin prepared in Example 1 and dissolve it in 4 mL of hexafluoroisopropanol, stir until completely dissolved to obtain 0.08 g / mL silk fibroin liquid A, and take 3.2 mL of graphene with a pipette gun Centrifuge the ethanol dispersion at high speed, suck out the ethanol, transfer the graphene in the centrifuge tube to the silk fibroin solution with hexafluoroisopropanol, add a certain amount of hexafluoroisopropanol to make the silk fibroin solution reach 6mL, and ultrasonicate for 60min. Finally, the 0.06g / mL silk fibroin spinning solution B whose mass ratio of graphene and silk fibroin is 1:100 is obtained, and the spinning solution is injected into a 10mL syringe for electrospinning, and the process conditions are: spinning voltage 16kv, pushing speed 0.05mm / min, receiving distance 12cm, roller speed 100r / min. The obtained electrospun membrane was treated with absolute ethanol for 4 hours, and then dried in a vacuum oven at 50° C. for 24 hours to...

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Abstract

The invention provides a preparation method of a silk fibroin/graphene composite nano fiber support material. The preparation method comprises the following steps: preparing a silk fibroin sample, subjecting a graphene ethanol dispersion liquid to high speed centrifugation to obtain graphene; adding obtained silk fibroin sample into hexafluoroisopropanol, stirring to completely dissolve the silk fibroin sample so as to obtain a solution A; transferring the obtained graphene into the solution A by using hexafluoroisopropanol, dispersing the graphene by ultrasonic waves to obtain a solution B; transferring the solution B to an injection syringe, carrying out electrostatic spinning by controlling the technical parameters of electrostatic spinning; carrying out a post treatment namely soaking obtained electrostatic spun films into anhydrous ethanol for four hours, and naturally drying to obtain the silk fibroin/graphene composite nano fiber support material. The preparation method is simple and feasible, the raw materials are abundant, and the industrial production can be realized easily. Micro/nano meter level composite fibers can be prepared; and the composite fiber preserves the good biocompatibility of silk fibroin and excellent biological activity and mechanical properties of graphene.

Description

technical field [0001] The invention belongs to the field of preparation of electrospinning composite nanofibers, and in particular relates to a preparation method of a silk fibroin / graphene composite nanofiber support material. Background technique [0002] Silk fibroin is degummed from silkworm cocoons. It is a natural protein with excellent properties such as good biocompatibility, biodegradability, good air permeability, and non-immunogenicity. It is widely used in skin, Repair and regeneration of bone, cartilage, tendon, nerve conduit and other tissues. [0003] The scaffold material prepared by electrospinning technology can effectively mimic the structural and functional characteristics of the human extracellular matrix (ECM), effectively solve the biocompatibility of the material, regulate the conduction of biological signals, and induce the growth of cells and tissues. Promote body regeneration and repair. [0004] Graphene is a carbon material with a two-dimensio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/50D04H1/728
CPCA61L27/443A61L27/50A61L2430/00D04H1/728C08L89/00
Inventor 赵政万玲李泽浩
Owner WUHAN UNIV OF TECH
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