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A hollow nanosphere used for loading lipase for vanillin preparation through catalytic oxidation

A hollow nano, catalytic oxidation technology, applied in the direction of microsphere preparation, biochemical equipment and methods, enzymes, etc., can solve the problems of single product aroma, environmental pollution, and inability to meet the needs of the world market, and achieve high activity effects

Inactive Publication Date: 2017-07-14
MUDANJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plant extraction method is limited by the supply of raw material vanilla pods, which makes the natural vanillin extracted only from vanilla far from meeting the demand of the world market
At present, the vast majority of vanillin products on the market are obtained through chemical synthesis, such as the condensation process of guaiacol and glyoxylic acid. Although the process equipment is simple and the product purity is high, there are serious environmental pollution defects and the product has a single aroma. , and the safety of preparing spices has also been questioned

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of polymer hollow nanospheres: add 40 parts of glucose methacrylate, 25 parts of maleic rosin ethylene glycol acrylate, 15 parts of nanoporogens and 1 part of benzoyl peroxide to 350 parts of water, Heat up to 60-65°C and mix for 15 minutes, then microwave at a microwave frequency of 2450MHz and an output power of 700W for 5 minutes, and stand at 0-5°C for 30 minutes. Stand in an environment of ℃ for 15 minutes, mix well and microwave again for 10 minutes, the resulting mixture is cooled to 45-50 ℃ at a cooling rate of 5 ℃ / min, after solid-liquid separation, the obtained spheres are washed with cold water at 10-15 ℃ three times, and finally Vacuum drying at 60-65°C to obtain polymer hollow nanospheres;

[0028] (2) Polymer-TiO 2 Preparation of composite hollow nanospheres: Add 15 parts of nano-titanium dioxide, 3 parts of microcrystalline cellulose cross-linked polyacrylamide and 1 part of hydrogenated rosin pentaerythritol ester to 25 parts of β-pinene...

Embodiment 2

[0034] (1) Preparation of polymer hollow nanospheres: add 50 parts of glucose methacrylate, 30 parts of maleic rosin ethylene glycol acrylate, 20 parts of nano-porogen and 1 part of azobisisobutyronitrile to 350 parts of water , heat up to 60-65°C and mix for 15 minutes, then microwave at a microwave frequency of 2450MHz and an output power of 700W for 10 minutes, and stand at 0-5°C for 30 minutes, mix well and continue to microwave for 5 minutes, and then in -5- Stand at 0°C for 15 minutes, mix well and microwave again for 10 minutes, cool the mixture to 45-50°C at a cooling rate of 5°C / min, wash the obtained spheres with 10-15°C cold water three times after solid-liquid separation, Finally, vacuum-dry at 60-65°C to obtain polymer hollow nanospheres;

[0035] (2) Polymer-TiO 2 Preparation of composite hollow nanospheres: Add 15 parts of nano-titanium dioxide, 3 parts of microcrystalline cellulose cross-linked polyacrylamide and 1 part of hydrogenated rosin pentaerythritol es...

Embodiment 3

[0058] The hollow nanospheres made by the same amount of Example 1, Example 2, Comparative Example 1, Comparative Example 2 and Comparative Example 3 are made into a lipase catalyst loaded with lipase Novozyme435 through the same loading process, and the loading capacity of lipase Novozyme435 is measured , and the prepared lipase catalyst was used for the catalytic oxidation synthesis of vanillin, and the yield and purity of the prepared vanillin were measured, and the results are shown in Table 1.

[0059] Preparation of lipase catalyst: 5 mL of lipase Novozyme435 buffer was added to 1 g of hollow nanospheres, shaken at 35° C. for 24 hours, centrifuged, and the spheres were taken and washed with water to obtain the lipase catalyst.

[0060] Catalytic oxidation synthesis of vanillin: Add 20mL toluene, 0.1mol coniferaldehyde and 0.5g lipase catalyst to the reaction flask, add 0.15mol oxalic acid under stirring, then add 0.15mol urea hydrogen peroxide in 5 batches, and finish add...

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PUM

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Abstract

A hollow nanosphere used for loading lipase for vanillin preparation through catalytic oxidation is disclosed and relates to the technical field of biological catalysis. The method includes (1) preparing a polymer hollow nanosphere; (2) preparing a polymer-TiO2 composite hollow nanosphere; (3) covering the composite hollow nanosphere with a hard shell; and (4) preparing the disclosed hollow nanosphere. The prepared hollow nanosphere is used for loading the lipase. The lipase loading amount in each gram of the hollow nanosphere is 15 mg or above. A prepared lipase catalyst is specially used for vanillin preparation through catalytic oxidation, coniferyl aldehyde is adopted as a raw material, hydrogen peroxide-urea is adopted as an oxygen source of the lipase, the yield of prepared vanillin is 93% or above, and purity of the prepared vanillin is 98% or above.

Description

[0001] Technical field: [0002] The invention relates to the technical field of biocatalysis, in particular to a hollow nanosphere for lipase loading of vanillin prepared by a catalytic oxidation method. [0003] Background technique: [0004] Vanillin, also known as vanillin and vanillin, is the main component of vanilla beans. It is one of the largest and most versatile spices in the world. It can be used not only as a flavor enhancer, but also in food. As preservatives, preservatives, antioxidants, flavoring agents or fixatives, but also as pharmaceutical intermediates for drug synthesis. [0005] At present, the production methods of vanillin mainly include plant extraction, chemical synthesis and biosynthesis. The plant extraction method is limited by the supply of raw material vanilla pods, which makes the natural vanillin only extracted from vanilla far from meeting the demand of the world market. At present, the vast majority of vanillin products on the market are ob...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/08B01J13/04B01J13/20C08F220/28C08F220/40
CPCB01J13/043B01J13/206C08F220/28C12N9/20C12N11/08C12N11/14C12Y301/01003C08F220/282C08F220/40
Inventor 孙凯徐红纳闫磊王妍桂金秋蔡克瑞刘春玲崔荣军韩彦龙晁岳刚
Owner MUDANJIANG MEDICAL UNIV
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