A hollow nanosphere used for loading lipase for vanillin preparation through catalytic oxidation

Abstract

A hollow nanosphere used for loading lipase for vanillin preparation through catalytic oxidation is disclosed and relates to the technical field of biological catalysis. The method includes (1) preparing a polymer hollow nanosphere; (2) preparing a polymer-TiO2 composite hollow nanosphere; (3) covering the composite hollow nanosphere with a hard shell; and (4) preparing the disclosed hollow nanosphere. The prepared hollow nanosphere is used for loading the lipase. The lipase loading amount in each gram of the hollow nanosphere is 15 mg or above. A prepared lipase catalyst is specially used for vanillin preparation through catalytic oxidation, coniferyl aldehyde is adopted as a raw material, hydrogen peroxide-urea is adopted as an oxygen source of the lipase, the yield of prepared vanillin is 93% or above, and purity of the prepared vanillin is 98% or above.

Description

[0001] Technical field:

[0002] The invention relates to the technical field of biocatalysis, in particular to a hollow nanosphere for lipase loading of vanillin prepared by a catalytic oxidation method.

[0003] Background technique:

[0004] Vanillin, also known as vanillin and vanillin, is the main component of vanilla beans. It is one of the largest and most versatile spices in the world. It can be used not only as a flavor enhancer, but also in food. As preservatives, preservatives, antioxidants, flavoring agents or fixatives, but also as pharmaceutical intermediates for drug synthesis.

[0005] At present, the production methods of vanillin mainly include plant extraction, chemical synthesis and biosynthesis. The plant extraction method is limited by the supply of raw material vanilla pods, which makes the natural vanillin only extracted from vanilla far from meeting the demand of the world market. At present, the vast majority of vanillin products on the market are ob...