A kind of immunoenhancing anti-tumor collagen composition, preparation method and application thereof
A technology of immune enhancement and composition, which is applied in the direction of antineoplastic drugs, drug combinations, and cancer antigen components, and can solve problems such as severe reactions, difficulties in achieving uniform standards in the production process, contact allergies, and granulomatous inflammation, and achieve effective results Good, retain active growth factors, mild decellularization process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0057] The immunoenhancing anti-tumor collagen composition of this example is prepared through the following steps:
[0058] (1) Organization pre-processing:
[0059] The submucosal tissue material of the small intestine was divided into the specified size of 10cm in width and 15cm in length, the lymphoid tissue was removed, rinsed with tap water 3 times, and then rinsed with purified water until the surface was free of stains, and then placed on a stainless steel filter and other water filtration devices, static Leave for 5 minutes or more and drain the water.
[0060] (2) Virus inactivation:
[0061] Use peracetic acid-ethanol solution to soak small intestinal submucosa tissue material for virus inactivation, and this process can be carried out in a stainless steel bucket. The volume percentage concentration of peracetic acid in peracetic acid-ethanol solution is 1%, the volume percentage concentration of ethanol is 24%, the volume ratio of peracetic acid-ethanol solution ...
Embodiment 2
[0077] Carry out performance test to sample in embodiment 1, test item and result are as follows:
[0078] 1) Identification of collagen subtypes: Immunohistochemical staining was used to detect collagen types I, III, IV and VI, serial sections with a thickness of 3 μm were dewaxed in xylene, and dehydrated in graded ethanol. Move the slices into a rice cooker water bath (containing 0.01mol / L, pH 6.0 trisodium citrate buffer solution), keep the temperature at 95-100°C, cook for 20min for antigen retrieval, take it out and let it cool naturally at room temperature. Phosphate buffered saline (PBS) washing, 5min×3 times. Two-step method for immunohistochemistry: Add the primary monoclonal antibodies to collagen types I, III, IV, and VI dropwise at a concentration of 1:100, incubate at 4°C for 60 minutes at room temperature overnight, and wash three times with PBS. Add Envision reaction solution dropwise and incubate at room temperature for 30 min. Wash 3 times with PBS. 0.05% ...
Embodiment 3
[0089] According to Example 1, the inactivated lung cancer cell compound immune enhancer sheet material was obtained after the mouse lung cancer cells were inactivated, and 0.1 ml was subcutaneously injected into the flanks of 3-month-old healthy experimental mice. 6 A lung cancer cell was used to create a tumor model. When the tumor was formed, tumor resection was performed to retain the tumor base. The animals were randomly divided into control group, SIS group, and inactivated lung cancer cell compound immune enhancer sheet material group, and the material was covered on the wound surface. The tumor was removed and fixed in the control group. After 3 weeks, the tumor recurrence, tumor weight, and tumor metastasis were observed on the wound after tumor resection. It was found that the recurrence rate of swelling and pain was 100%. It can slow down the tumor growth rate, but cannot control the tumor metastasis. However, after compounding the inactivated lung cancer cells and S...
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
thickness | aaaaa | aaaaa |
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com