A kind of immunoenhancing anti-tumor collagen composition, preparation method and application thereof

A technology of immune enhancement and composition, which is applied in the direction of antineoplastic drugs, drug combinations, and cancer antigen components, and can solve problems such as severe reactions, difficulties in achieving uniform standards in the production process, contact allergies, and granulomatous inflammation, and achieve effective results Good, retain active growth factors, mild decellularization process

Active Publication Date: 2021-01-05
BEIJING BIOSIS HEALING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the only enhancer approved by the FDA for human immunity is aluminum salt adjuvant, and due to the high heterogeneity of the material, it is difficult to achieve a unified standard in the production process of the adjuvant, and subcutaneous injection is likely to cause serious reactions (such as erythema, subcutaneous nodules, etc.) knots, contact allergy and granulomatous inflammation)

Method used

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  • A kind of immunoenhancing anti-tumor collagen composition, preparation method and application thereof
  • A kind of immunoenhancing anti-tumor collagen composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The immunoenhancing anti-tumor collagen composition of this example is prepared through the following steps:

[0058] (1) Organization pre-processing:

[0059] The submucosal tissue material of the small intestine was divided into the specified size of 10cm in width and 15cm in length, the lymphoid tissue was removed, rinsed with tap water 3 times, and then rinsed with purified water until the surface was free of stains, and then placed on a stainless steel filter and other water filtration devices, static Leave for 5 minutes or more and drain the water.

[0060] (2) Virus inactivation:

[0061] Use peracetic acid-ethanol solution to soak small intestinal submucosa tissue material for virus inactivation, and this process can be carried out in a stainless steel bucket. The volume percentage concentration of peracetic acid in peracetic acid-ethanol solution is 1%, the volume percentage concentration of ethanol is 24%, the volume ratio of peracetic acid-ethanol solution ...

Embodiment 2

[0077] Carry out performance test to sample in embodiment 1, test item and result are as follows:

[0078] 1) Identification of collagen subtypes: Immunohistochemical staining was used to detect collagen types I, III, IV and VI, serial sections with a thickness of 3 μm were dewaxed in xylene, and dehydrated in graded ethanol. Move the slices into a rice cooker water bath (containing 0.01mol / L, pH 6.0 trisodium citrate buffer solution), keep the temperature at 95-100°C, cook for 20min for antigen retrieval, take it out and let it cool naturally at room temperature. Phosphate buffered saline (PBS) washing, 5min×3 times. Two-step method for immunohistochemistry: Add the primary monoclonal antibodies to collagen types I, III, IV, and VI dropwise at a concentration of 1:100, incubate at 4°C for 60 minutes at room temperature overnight, and wash three times with PBS. Add Envision reaction solution dropwise and incubate at room temperature for 30 min. Wash 3 times with PBS. 0.05% ...

Embodiment 3

[0089] According to Example 1, the inactivated lung cancer cell compound immune enhancer sheet material was obtained after the mouse lung cancer cells were inactivated, and 0.1 ml was subcutaneously injected into the flanks of 3-month-old healthy experimental mice. 6 A lung cancer cell was used to create a tumor model. When the tumor was formed, tumor resection was performed to retain the tumor base. The animals were randomly divided into control group, SIS group, and inactivated lung cancer cell compound immune enhancer sheet material group, and the material was covered on the wound surface. The tumor was removed and fixed in the control group. After 3 weeks, the tumor recurrence, tumor weight, and tumor metastasis were observed on the wound after tumor resection. It was found that the recurrence rate of swelling and pain was 100%. It can slow down the tumor growth rate, but cannot control the tumor metastasis. However, after compounding the inactivated lung cancer cells and S...

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Abstract

The invention provides an immune-enhancing antineoplastic collagen composition as well as a preparation method and an application thereof. The composition is characterized by consisting of antineoplastic factors, collagen, polysaccharide substances and active factors. The composition is applicable to, but not limited in, cells for treating breast cancer, colorectal cancer, ovarian cancer, pancreatic cancer, prostatic cancer, bladder cancer, melanoma, lung cancer, stomach cancer, liver cancer, head and neck squamous cell carcinoma, carcinoma of uterine cervix, renal cell carcinoma, thyroid cancer, acute myeloid leukemia, myeloma, esophageal squamous cancer, lymphoma and the like.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to an immune-enhancing anti-tumor collagen composition, a preparation method and its application. The composition can be used for tumor prevention, postoperative tumor recurrence prevention or prolonging the survival period of tumor patients. Background technique [0002] Malignant tumors have become the "No. 1 killer" that threatens human health and life. Among the causes of human death, the number of deaths from malignant tumors has risen to the top. About 8.7 million people in the world suffer from tumors every year, and about 6.9 million people die. In my country alone, 1.05 million people die of advanced malignant tumors every year. At present, the methods for treating malignant tumors are very limited, and tumor vaccines are another immunotherapy program following surgical resection, chemotherapy, and radiotherapy, including therapeutic and preventive vaccines. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61L27/38A61L27/36A61L27/54A61P35/00A61K31/728A61K31/737A61K38/17A61K38/18A61K38/39
CPCA61K31/728A61K31/737A61K38/1777A61K38/18A61K38/39A61K39/0011A61K2039/5152A61L27/3629A61L27/3687A61L27/3804A61L27/3895A61L27/54A61L2300/256A61L2300/416A61K2300/00
Inventor 赵博夏磊磊王洪权赵延瑞李学军张晋辉
Owner BEIJING BIOSIS HEALING BIOLOGICAL TECH
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