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Detection method for narcotics in human body fluid based on gold nanorods SERS substrate

A gold nanorod and detection method technology, applied in the field of SERS substrate cleaning, can solve problems such as reducing the background signal of gold nanorods, and achieve the effects of high sensitivity, background signal reduction and reproducibility improvement

Inactive Publication Date: 2017-08-11
安徽中科赛飞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When synthesizing gold nanorods, the protective agent cetyltrimethylammonium bromide (CTAB) needs to be added, and CTAB has a strong SERS peak. Adding sodium borohydride solution to the gold nanorod sol, Au—H will Substituting Au-N, which will greatly reduce the background signal of gold nanorods, thus providing the possibility of detecting low concentrations of drugs

Method used

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  • Detection method for narcotics in human body fluid based on gold nanorods SERS substrate
  • Detection method for narcotics in human body fluid based on gold nanorods SERS substrate
  • Detection method for narcotics in human body fluid based on gold nanorods SERS substrate

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Embodiment 1

[0058] A method for detecting drugs in human body fluids based on a gold nanorod SERS substrate, comprising the following steps:

[0059] (1) Synthesis of SERS-active gold nanorod sols with different aspect ratios;

[0060] (2) The gold nanorod sol was mixed with 0.1 M sodium borohydride, ultrasonically oscillated, and then the mixture was centrifugally cleaned;

[0061] (3) Disperse the cleaned mixture in the stabilizer sodium citrate solution and place it stably, then take the dispersion and wash it by centrifugation, and finally drop it on a clean silicon wafer, and obtain the SERS substrate after drying;

[0062] (4) Take 0.8mL of human urine, add 0.2mL of acetone to it, mix well and centrifuge, and take the supernatant;

[0063] (5) Add sodium hydroxide to the supernatant, shake well, then add pentane, shake and extract; centrifuge the extract, and take its organic layer solution;

[0064] (6) Drop the organic layer solution onto the SERS substrate in step (3), and use ...

Embodiment 2

[0070] (1) Synthesis of SERS-active gold nanorod sols with different aspect ratios;

[0071] (2) Mixing the gold nanorod sol and hydroxylamine hydrochloride, ultrasonically oscillating, and then centrifugally cleaning the mixture;

[0072] (3) Disperse the cleaned mixture in the stabilizer tannic acid solution for stable placement, then take the dispersion and wash it centrifugally, and finally drop it on a clean silicon wafer, and obtain the SERS substrate after drying;

[0073] (4) Take 0.8 mL of human urine, add 0.6 mL of acetone to it, mix well and centrifuge, and take the supernatant;

[0074] (5) Add carbonate buffer solution to the upper supernatant, shake well, then add cyclohexane, shake and extract; after centrifuging the extract, take its organic layer solution;

[0075] (6) Drop the organic layer solution onto the SERS substrate in step (3), and use a Raman spectrometer with a light wavelength of 785 nm for SERS detection.

Embodiment 3

[0077] (1) Synthesis of SERS-active gold nanorod sols with different aspect ratios;

[0078] (2) The gold nanorod sol was mixed with 0.1 M sodium borohydride, ultrasonically oscillated, and then the mixture was centrifugally cleaned;

[0079] (3) Disperse the cleaned mixture in the stabilizer polyethylene glycol for stable placement, then take the dispersion liquid for centrifugal cleaning, and finally drop it on a clean silicon wafer, and obtain the SERS substrate after drying;

[0080] (4) Take 0.8mL of human urine, add 0.4mL of acetone to it, mix well and centrifuge, and take the supernatant;

[0081] (5) Add ammonia water to the supernatant liquid, shake well, then add chloroform, shake and extract; after centrifuging the extract, take its organic layer solution;

[0082] (6) Add the organic layer solution dropwise to the SERS substrate in step (3), and the organic phase can be completely volatilized in about 15 seconds, and the SERS detection is performed using a Raman s...

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Abstract

The invention discloses a detection method for narcotics in human body fluid based on a gold nanorods SERS substrate. The method comprises the steps that gold nanorod sols with different draw ratios and SERS activity are synthesized; the gold nanorod sols and a cleaning agent are mixed, ultrasonically oscillated, centrifugally cleaned and then dispersed into a stabilizing agent, the mixture is dropped on a clean silicon wafer after being centrifugally cleaned, and the SERS substrate is obtained after drying is performed; human urine is taken, an organic solution is added into the human urine to be mixed to be uniform, then, centrifuging is performed, an alkali solution is added into a supernatant, an organic extraction agent is added after uniform shaking, vibration extraction and centrifuging are performed, and then an organic layer solution is taken; the organic layer solution is dropped on the SERS substrate, a raman spectrometer is used for performing SERS detection. After the gold nanorod sols are cleaned with the cleaning agent, the influence of background signals is lowered, and both the detection sensitivity and the reproducibility are improved.

Description

technical field [0001] The invention relates to a method for cleaning a SERS substrate, in particular to a method for detecting drugs in human body fluids based on a gold nanorod SERS substrate. Background technique [0002] Drugs are a hot topic. At present, the situation of drug flooding in the world is severe, and my country is no exception. Especially in recent years, as my country has become the world's largest trading country, commodity circulation has become more frequent, and drugs often enter the drug trading market through various logistics channels. Among them, as the most widely used synthetic drugs, amphetamine drugs represented by ecstasy and methamphetamine, and ketamine are more localized and their production and consumption are increasing year by year. However, in the process of drug trade and drug use, it is often difficult to detect due to relatively concealed or less residual amount, which brings certain obstacles to the fight against drug crimes. The...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28
CPCG01N21/658G01N1/28
Inventor 杨良保毛妹唐祥虎陈诚于博荣孟娟曹晓敏董荣录
Owner 安徽中科赛飞尔科技有限公司
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