Streptococcus salivarius and its application in the preparation of drugs for removing halitosis

A technology of halitosis and medicine, applied in the field of microorganisms, to achieve the effect of wide application prospects and value

Active Publication Date: 2020-04-03
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The researchers proposed that S. salivarius K12 can be used as an effective adjuvant treatment in the treatment of halitosis, but long-term and periodic intake of preparations containing S. salivarius K12 is required to inhibit oral odor-producing bacteria

Method used

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  • Streptococcus salivarius and its application in the preparation of drugs for removing halitosis
  • Streptococcus salivarius and its application in the preparation of drugs for removing halitosis
  • Streptococcus salivarius and its application in the preparation of drugs for removing halitosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Screening of Example 1 Strain

[0020] Implementation goal: To isolate the dominant oral flora from the saliva of 250 children without halitosis, and to detect the decomposition of sulfur-containing compounds, to find natural bacteria that can inhibit halitosis.

Embodiment approach

[0022] (1) Sampling: 250 normal children aged 4-7 without bad breath and oral diseases, no family genetic diseases, no systemic diseases, and no long-term medication history. Before saliva collection, the purpose and process of the experiment were explained to the school and parents, and consent was obtained. Children should not eat or drink within 2 hours after breakfast. With a 15ml sterile sample collection tube, about 2ml of saliva is naturally collected without external stimulation of saliva secretion. Each sample is divided into three tubes, which are respectively used for PCR identification, bacterial culture and storage.

[0023] (2) Separation and culture: All samples were separated and cultured, diluted with PBS, and evenly spread on the brain heart infusion BHI petri dish, placed at 37°C, 5% CO 2 incubator for cultivation.

[0024] (3) Identification by matrix-assisted laser desorption tandem time-of-flight mass spectrometer (MALDI-TOF MS):

[0025] Sample proces...

Embodiment 2

[0087] Example 2. Use and effect of bacterial strains to prevent halitosis

[0088] Decomposition ability of dominant Streptococcus salivarius to volatile sulfide produced by standard halitosis strains:

[0089] By detecting the concentration of hydrogen sulfide and methyl mercaptan in the gas above the co-culture solution of Clostridium nucleatum and Streptococcus salivarius. Gas chromatography was used to detect the sulfur-containing gas concentration of hydrogen sulfide and methyl mercaptan in the upper gas after co-cultivation of Clostridium nucleatum and Streptococcus salivarius. The results show that there are higher concentrations of hydrogen sulfide and methyl mercaptan ( Figure 4 ( Figure 5 ).

[0090] Table 1 is the specific detection value

[0091] Table 1. HPLC detection of sulfur-containing gas concentrations

[0092]

[0093] These results suggest that the dominant Streptococcus salivarius has a distinct ability to decompose hydrogen sulfide and methyl ...

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Abstract

The invention provides an oral probiotic strain, the preservation number of which is CGMCC NO.12899, the preservation date is August 23, 2016, the preservation classification is named Streptococcus salivarius (Streptococcus Salivarius), and the preservation unit is China Microbiology The General Microbiology Center of the Culture Collection Management Committee is located at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacterial strain can obviously inhibit the production of volatile sulfide of the bad breath standard strain Clostridium nucleatum. The invention also discloses the application of the above bacterial strains in the preparation of medicines for removing halitosis, which has broad application prospects and value in the preparation of microecological preparations for removing halitosis and constructing beneficial oral microecological environment effects.

Description

technical field [0001] The invention relates to a probiotic and its application, belonging to the field of microorganisms. Background technique [0002] Bad breath, a symptom of a foul odor when breathing, is a common disease, with prevalence in Chinese ranging from 22% to more than 50%. Patients with bad breath do not dare to communicate with others in close proximity, resulting in inferiority complex and affecting normal interpersonal and emotional communication, which is very distressing. Bad breath is mainly caused by bacteria in the oral cavity, especially the volatile sulfide (Volatile Sulphur Compound, VSC) produced by the fermentation of Gram-negative anaerobic bacteria accumulated in the posterior 1 / 3 of the tongue. Studies have shown that VSCs are mainly produced by bacteria such as Porphyromonas gingivalis, Stenabacter forsythia, Prevotella intermedia and Fusobacterium nucleatum. Kazor et al. used PCR to analyze the bacteria in the oral cavity of healthy and hal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/744A61P1/02C12R1/46
CPCA61K35/744A23V2002/00C12R2001/46C12N1/205A23V2400/245A23V2200/30
Inventor 孙丽娜徐建国李振军
Owner ICDC CHINA CDC
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