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Preparation process and structure sequence of highland barley beta-glucan

A preparation process and glucan technology are applied to the preparation process of highland barley β-glucan and the field of its structure sequence, and can solve the problems of unclear medicinal properties, complex sequence of highland barley β-glucan, and difficulty in extracting purity. , to achieve the effect of good hypoglycemic and hypolipidemic function, high purity and simple preparation process

Inactive Publication Date: 2017-09-29
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation process of highland barley β-glucan and the structural sequence of highland barley β-glucan, so as to solve the problem of difficult extraction of highland barley β-glucan with high purity, complex sequence of highland barley β-glucan, Its medicinal properties are not clear, etc.

Method used

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  • Preparation process and structure sequence of highland barley beta-glucan
  • Preparation process and structure sequence of highland barley beta-glucan
  • Preparation process and structure sequence of highland barley beta-glucan

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preparation example Construction

[0043] The invention provides a preparation process of highland barley β-glucan, comprising the following steps:

[0044] (1) Highland barley crushing: after the highland barley is ground into powder, pass through a 40-80 mesh sieve to obtain highland barley powder;

[0045] (2) Enzyme inactivation: add high-concentration ethanol to the highland barley powder, and heat to reflux;

[0046] (3) Extraction and starch removal: add water to the highland barley powder processed in step (2), adjust the pH value to 7-11, then add high-temperature amylase, mix well, and then stir and reflux at 85-90°C 1-10h;

[0047] (4) Protein removal: adjust the pH value of the solution obtained in step (3) to 2-4.5, and after standing still, the protein precipitates out, and the precipitate is removed by centrifugation to obtain an extract;

[0048] (5) Preparation of crude polysaccharide: Concentrate the extract by 1-5 times, add ammonium sulfate, and precipitate the crude polysaccharide after s...

Embodiment 1

[0074] This implementation case shows a preparation process of highland barley β-glucan according to the following steps:

[0075] Grind the highland barley with bran and pass through a 60-mesh sieve; after collecting the highland barley powder, weigh 30 g of the highland barley powder, add 150 mL of 80% ethanol solution, and reflux at 75°C for 2 hours. Centrifuge to remove ethanol solution and pigment, and dry the precipitate in a drying oven. Add water and stir, according to the ratio of material to water is 1:15, add 450mL aqueous solution to 30g highland barley flour, use 20%Na 2 CO 3 Adjust the pH to 10, add 4.5mL (about 135U) of high-temperature-resistant amylase, stir and reflux at 90°C (85-95°C is acceptable) for 2h, 4500r / min, 15min, collect the supernatant; cool to room temperature, 4500r / min, 15min, after separation, collect the supernatant, then add an appropriate amount of high-temperature-resistant amylase, keep at 85°C for 1-2h, use 0.1mol / L iodine solution as...

Embodiment 2

[0123] This implementation case shows a preparation process of highland barley β-glucan according to the following steps:

[0124] Grind the highland barley with bran and pass through a 40-mesh sieve; after collecting the highland barley powder, weigh 80 g of the highland barley powder, add 400 mL of 80% ethanol solution, and reflux at 75°C for 2 hours. Centrifuge to remove ethanol solution and pigment, and dry the precipitate in a drying oven. Add water and stir, according to the ratio of material to water is 1:20, add 1600mL aqueous solution to 80g highland barley powder, use 20%Na 2 CO 3 Adjust the pH to 10, add 12mL (about 359U) of high temperature resistant amylase, stir and reflux at 85°C for 2h, 4500r / min, 15min, collect the supernatant; cool to room temperature, 4500r / min, 15min, collect after separation Add an appropriate amount of high-temperature-resistant amylase to the supernatant, keep at 85°C for 1-2 hours, and use 0.1mol / L iodine solution as an indicator unti...

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Abstract

The invention discloses a preparation process and a structure sequence of highland barley beta-glucan. The preparation process of the highland barley beta-glucan comprises the following steps: crushing highland barley, performing enzyme deactivation, removing starch, removing proteins, preparing crude polysaccharides, removing pigments, removing small molecules, and drying, thereby obtaining the highland barley beta-glucan. As the novel highland barley beta-glucan is separated from the highland barley, the structure and sequence of the beta-glucan are analyzed by using ion chromatography, an ampere pulse detector and multiple methods with on-line high resolution mass spectrum (HPAEC-PAD / QTOF-MS) as main means, in-vivo tests show that the highland barley beta-glucan which is prepared by using the method and has specific structures and sequences has blood sugar and blood fat reduction activity, and a base is made for developing new medicines for treating hyperlipidemia and diabetes.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a preparation process of highland barley β-glucan and its structural sequence. Background technique [0002] Highland barley, belonging to the genus Barley, is one of the staple foods of the people in the Qinghai-Tibet Plateau region, as well as livestock feed and industrial raw material crops. According to monitoring, the content of β-glucan in highland barley is the crop with the highest content among all grains. β-glucans in cereals are linear polysaccharides formed by glucopyranose linked by β-(1→3) or β-(1→4) glycosidic bonds. In recent years, highland barley β-glucan has received great attention in the field of food science, and many important physiological functions and physiological activities of β-glucan have gradually been recognized. It is reported that highland barley β-glucan can be used as an intrinsic The modulator of immunity and acquired immunity has a goo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0024
Inventor 张真庆戎银秀徐乃玉李笃信
Owner SUZHOU UNIV
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