Immortalized rabbit small intestine epithelium cell line and construction method thereof

A small intestinal epithelial cell and construction method technology, applied in the field of immortalized rabbit small intestinal epithelial cell line and its construction, can solve the problems of restricting the development of technology in the field and the lack of scientific tools
CN107217040APending Publication Date: 2017-09-29CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2017-09-29

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Abstract

The invention relates to the field of molecular biology and specifically discloses an immortalized rabbit small intestine epithelium cell line and a construction method thereof. The small intestines of a newly born rabbit are collected, the enzymatic digestion juice containing collagenase and neutral protease is utilized to digest, and after the digestion, a method for re-suspending centrifuging and differential adherence is adopted for purifying the pit cell cluster, so that high-purity primary pit epithelium cells can be obtained. The pit epithelium cells can participate in culture and passage. The obtained pit epithelium cells are used for performing liposome transfection expression on the SV40 big T antigenic plasmid, the passage is continued, and the immortalized rabbit small intestine epithelium cell line is finally screened. The immortalized rabbit small intestine epithelium cell line can simultaneously express intestine epithelium surface marker molecules and pit basic stem cell surface molecules. The intestine epithelium surface marker molecules include but not limited to keratin 8, E-calcitonin and ki67; and the pit basic stem cell surface molecules include but not limited to Lgr5 and sox9. The immortalized rabbit small intestine epithelium cell line can be used for establishing a rabbit small intestine epithelium in vitro model and can be used for researching a nutrition absorbing mechanism, cellular pathway excavation and pathogen in vitro culture.
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Description

technical field

[0001] The invention relates to the field of molecular biology, in particular to an immortalized rabbit small intestinal epithelial cell line and a construction method thereof. Background technique

[0002] Rabbit is an important source of meat and fur, and plays an important role in livestock production, catering, public health and other fields. At present, major epidemic diseases of rabbits such as rabbit plague, rabbit coccidiosis, pasteurellosis, etc. have been paid more and more attention. Most of these pathogens attack the digestive tract. However, in the current research, scientific tools to study these diseases are seriously lacking, which restricts the technological development in related fields. At present, there are few cell lines derived from rabbits, so obtaining more cell lines derived from rabbits will promote the research on the prevention and control of major rabbit diseases.

[0003] Cell lines derived from the small intestine are reliabl...

Claims

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