Clostridium beijerinckii producing electricity, construction method, and application thereof

A technology of Clostridium beijerinckii and Clostridium beijerinckii, which is applied in the field of improving the electricity production of Clostridium beijerinckii, can solve the problems of weak microbial electricity production ability and unclear specific mechanism, and achieve high-efficiency electricity production and increase electricity production. Effect

Active Publication Date: 2017-10-24
南京中环生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Scientific researchers have initially revealed the mechanism of microbial electricity generation, but the specific mechanism is still unclear. The ability o

Method used

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  • Clostridium beijerinckii producing electricity, construction method, and application thereof
  • Clostridium beijerinckii producing electricity, construction method, and application thereof
  • Clostridium beijerinckii producing electricity, construction method, and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: Construction method of Clostridium beijerinckii Cbei_2996 gene inactivation mutant

[0029] (1) Design introns:

[0030] According to the Cbei_2996 gene sequence (as shown in SEQ ID No: 1) of Clostridium beijerinckii included in the NCBI database, that is, the base sequence (nuoA) encoding coenzyme Q oxidoreductase subunit A, a suitable insertion gene site is designed with the help of software point( http: / / www.clostron.com ), through the previous experimental screening and verification, it was selected to be inserted between the 62nd and 63rd bases of the Cbei_2996 gene sequence, and an intron sequence was generated. The synthesized intron was S-62, and its sequence was shown as SEQ ID NO: 2.

[0031] (2) Construction of Cbei_2996 insertion inactivation vector:

[0032] Use XhoI and BsrGI to double-enzyme-cut the vector pWJ (provided by Mr. Yang Sheng, Shanghai Academy of Biological Sciences, its sequence is shown in SEQ ID NO: 5, and the plasmid map ...

Embodiment 2

[0044] This example illustrates the electricity production experiment using 1 g / L glucose by Clostridium beijerinckii as an anode catalyst.

[0045] (1) Construction of microbial fuel electricity:

[0046] In this embodiment, a microbial fuel cell using Clostridium beijerinckii to generate electricity as an anode catalyst is established according to existing technologies and methods, as shown in the attached figure 2 As shown, it includes four parts: anode chamber, cathode chamber, proton exchange membrane and external circuit. The anode electrode and cathode electrode are PAN-based graphite soft felt (5×5cm), with titanium wire as the external circuit, the external resistance is 1000Ω, the proton exchange membrane is DuPont NafionN117, and the data collector is Keithley series.

[0047] (2) Culture medium formula:

[0048] YPS seed medium: yeast powder 3g / L, peptone 5g / L, glucose 1g / L, ammonium acetate 2g / L, sodium chloride 3g / L, magnesium sulfate heptahydrate 3g / L, potass...

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Abstract

The invention discloses a strain of Clostridium Beijerinckii producing electricity. A Cbei-2996 gene in the Clostridium Beijerinckii is inactivated and cannot be normally expressed in the Clostridium Beijerinckii. The invention also discloses a construction method and an application of the high-electricity-producing recombinant Clostridium Beijerinckii. In a 1g/L glucose seed solution with 0.1 g/L of methyl viologen (MV) being an electronic mediator, the recombinant strain 2996 can reach 194 mV in maximum output voltage, while under the same conditions, an original strain 8052 only can reach 85 mV in the maximum output voltage; the maximum output voltage of the recombinant strain 2996 is 2.3 times as that of the original strain 8052. The method has simple operations and low cost, can effectively increase the output voltage of the Clostridium Beijerinckii. The recombinant strain 2996 is an excellent strain for researching and application of microbial fuel cells.

Description

technical field [0001] The invention belongs to the technical field of environment and new energy, and in particular relates to a method for increasing electricity production by Clostridium bejejejerinckii. Background technique [0002] Microbial Fuel Cell (MFC) is a device that uses electrogenic microorganisms as an anode catalyst to directly convert chemical energy in organic matter into electrical energy. (Bioresource Technol., 2009, 101(6):1533–1543). At present, microbial fuel cell (Microbial Fuel Cell, MFC) technology is becoming more and more mature, and has been paid attention to. Microbial fuel cells have potential applications in alternative energy sources, biosensors and wastewater treatment (Microb Ecol., 2004, 48(2): 178-190). [0003] At present, many electrogenic microorganisms have been found, such as Shewanella, Geobacter, Klebsiella, etc. Most of the electrogenic microorganisms are anaerobic microorganisms, and most of them are Gram-negative bacteria. The...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/74H01M8/16C12R1/145
CPCC12N9/0036C12Y106/05003H01M8/16Y02E60/50Y02P70/50
Inventor 郭亭朱刚强李永席梓淇
Owner 南京中环生物科技有限公司
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