A method for cultivating actinidia anthers into haploid plants

A technology of kiwi fruit and haploid, which is applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of long cycle and low efficiency of hybrid breeding, achieve simple operation, improve selection and breeding materials Effect

Active Publication Date: 2019-08-06
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Actinidia jujube is generally tetraploid, with 116 chromosomes (2n=116, 4X), highly heterozygous genes, and rich genetic diversity. The traditional hybrid breeding cycle is long, the efficiency is low, and there is great uncertainty. Therefore, it is almost impossible to obtain homozygous plants through traditional cross breeding

Method used

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  • A method for cultivating actinidia anthers into haploid plants
  • A method for cultivating actinidia anthers into haploid plants
  • A method for cultivating actinidia anthers into haploid plants

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Effect test

Embodiment 1

[0033] 1) Experimental material: Select 'Kuilu' plants that grow robustly and are free from diseases and insect pests. From 9:00 to 11:00 in the morning on a sunny day, the immature flower buds of "Kuilu" were collected, and the microspore development period was observed by the acetic acid magenta staining method. use;

[0034] 2) Low temperature treatment: put the collected flower buds in an environment of 2°C for pretreatment for 1 day. After the pretreatment, flower buds in the mononuclear marginal stage are selected respectively, and after disinfection and sterilization, the anthers are stripped. Anthers were inoculated on callus induction medium. 5-7 anthers were inoculated in each bottle, 10 bottles were inoculated for each treatment, and repeated 3 times. Cultivate in the dark for 20 days at 23°C;

[0035] 3) Induction of plant regeneration: when the induced callus grows to a diameter of 3-5 mm, it is transferred to the plant differentiation medium of Actinidia jujub...

Embodiment 2

[0039] 1) Experimental material: Select 'Kuilu' plants that grow robustly and are free from diseases and insect pests. From 9:00 to 11:00 in the morning on a sunny day, the immature flower buds of "Kuilu" were collected, and the microspore development period was observed by the acetic acid magenta staining method. use;

[0040] 2) Low temperature treatment: put the collected flower buds in an environment of 4°C for pretreatment, Process 1d, 3d, 5d, 7d, 10d After the pretreatment, the flower buds in the mononuclear stage were selected, rinsed with tap water for 30 minutes, and under aseptic conditions, the flower buds were sterilized with 70wt% alcohol for 30 seconds, rinsed with sterile distilled water for 3 to 4 times, and washed with 0.09wt% HgCl 2 The solution was sterilized for 5 minutes, rinsed with sterile distilled water for 3 to 4 times, and the anthers were stripped off. The anthers were inoculated on callus induction medium (MS+2,4-D 0.5 mg / L+6-BA 1.0 mg / L+sucrose ...

Embodiment 3

[0049] 1) Experimental material: Select 'Kuilu' plants that grow robustly and are free from diseases and insect pests. From 9:00 to 11:00 in the morning on a sunny day, the immature flower buds of "Kuilu" were collected, and the development of microspores was observed by acetic acid magenta staining. Tetrad, single-core early, single-core to the side inflorescence apical bud , collecting the flower buds at the top of the inflorescence where the megaspore development is in the uninucleate marginal stage for several days, and set aside;

[0050] 2) Low-temperature treatment: put the collected flower buds in an environment of 2°C for pretreatment for 5 days. After the pretreatment, select the flower buds in the single-nucleus marginalization stage, wash them with tap water for 30 minutes, and wash the flower buds with 75wt% alcohol under aseptic conditions. Disinfect for 30s, rinse with sterile distilled water 3 to 4 times, and use 0.12wt% HgCl 2 The solution was sterilized f...

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Abstract

The invention provides a method for cultivating actinidia jujube anthers into haploid plants, comprising the following steps: (A) collecting flower buds developed by microspores of actinidia jujubae, performing low-temperature cold storage for 1-10 days, and peeling off the anthers; (B) The anthers are placed in the callus induction medium and cultured in the dark for more than 20 days, then cultured on the plant differentiation medium for more than 40 days, and finally transferred to the rooting medium to cultivate roots. The method for cultivating Actinidia jujube anthers into haploid plants according to the embodiments of the present invention provides a variety of homozygous breeding materials for Actinidia jujube breeding, improves selection and breeding materials, and also provides an ideal foundation for basic theoretical research. acceptor material, and the anther culture method of the present invention is easy to operate compared to isolated microspore culture, somatic cell chromosome elimination, etc., so artificially cultivating isolated anthers is an effective way for Actinidia jujube to obtain haploid plants, which is very It is worth widely popularizing and applying.

Description

technical field [0001] The invention relates to the field of kiwifruit cultivation, in particular to a method for cultivating anthers of kiwifruit into haploid plants. Background technique [0002] Jujube kiwifruit, also known as soft jujube, kiwi pear, vine melon, vine pear, belongs to Actinidiaceae, and kiwifruit is a large deciduous vine plant. The fruit is small, smooth, and the whole fruit is edible. It is one of the most cold-resistant species in the genus Actinidia. one. Jujube kiwifruit enjoys the reputation of "the king of fruits". It is favored by consumers for its excellent quality, flavor, rich nutritional content, high medical and health care effects, and good processing suitability. Actinidia jujube is a promising small berry tree species in many regions of the world, especially in cold regions. The surface of kiwi fruit is smooth and hairless, with a lot of juice and good flavor. The pulp is rich in vitamins and amino acids, and the content of vitamin C is p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 秦红艳王广富艾军孙丹范书田杨义明许培磊刘迎雪赵滢王振兴张宝香李昌禹
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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