Small pet eye drops containing human mesenchymal stem cell excreta and preparation method thereof

A mesenchymal stem cell and secretion technology, which is applied in the field of eye drops containing human mesenchymal stem cell secretion and its preparation, can solve the problems of harsh survival conditions, discounts, and easy-to-death living cells of active cells, so as to shorten the healing time , Eliminate inflammation, promote the effect of repair

Inactive Publication Date: 2017-12-01
TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, due to the harsh survival conditions of active cells, it is easy to die, so the role played by living cells will be greatly reduced.

Method used

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  • Small pet eye drops containing human mesenchymal stem cell excreta and preparation method thereof
  • Small pet eye drops containing human mesenchymal stem cell excreta and preparation method thereof
  • Small pet eye drops containing human mesenchymal stem cell excreta and preparation method thereof

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preparation example Construction

[0020] The preparation method of the small pet eye drops containing human mesenchymal stem cell secretion of the present invention comprises the following steps:

[0021] (1) Mesenchymal stem cell culture: P2-P6 mesenchymal stem cells were selected from the cell bank for resuscitation, the inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was the maximum fusion 50%-60%, 35%-45%, 20%-30% of the temperature; the cells are inoculated and cultured in a carbon dioxide incubator at a temperature of 37°C and a volume ratio of carbon dioxide of 5%;

[0022] (2) When the fusion degree of the first batch of cells reaches 70%-80%, wash the cells twice with normal saline, and perform starvation culture with compound electrolyte injection;

[0023] (3) After 12-24 hours of starvation culture, the first batch of cell confluence reaches 90%-95%, collect the cell culture supernatant, centrifuge at 3000-4500g for 5-8min to ...

Embodiment 1

[0032] (1) Human umbilical cord mesenchymal stem cell culture: P2 human umbilical cord mesenchymal stem cells were selected from the cell bank for recovery, the total number of cells was 4.2*10 7 . The inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was 60% of the maximum fusion degree (the number of inoculated cells per bottle: 9*10 6 , three bottles of cells inoculated), 40% (the number of cells inoculated in each bottle: 6*10 6 , two bottles of inoculated cells), 20% (number of inoculated cells: 3*10 6 , inoculate a bottle of cells), the culture system is 40ml. The complete medium used for culturing the cells is phenol red-free DMEM / DF12 and 10% fetal calf serum by volume, and the bottle for inoculating the cells is a T-175 culture flask. After the cell inoculation is completed, place it in a carbon dioxide incubator for cultivation at a temperature of 37°C and a volume ratio concentration of carbon...

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Abstract

The invention discloses small pet eye drops containing human mesenchymal stem cell excreta and a preparation method thereof. The human mesenchymal stem cells can secrete various kinds of a large amount of cell factors in the cultivating process, the cell factors can penetrate deep into skin base layer to promote differentiation of skin tissue, neovascularization and growth of granulation tissue, promote structural reconstruction and regeneration and repair of injured skin tissue, promote wound surface healing and repair of eye conjunctiva and cornea. The cell factors and immunocompetence substances can adjust the microenvironment, and reduce bacterial infection and inflammatory reaction of eye conjunctiva and cornea tissue. Human mesenchymal stem cell excreta in the pet eye drops is extracted after repeated hunger cultivation, and contains the ingredients such as multiple electrolytes injection. The small pet eye drops can be applied to repairing of conjunctival and corneal ulcers.

Description

technical field [0001] The invention relates to stem cell secretions, in particular to an eye drop containing human mesenchymal stem cell secretions and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. They have the ability of self-renewal, tissue repair, and immune regulation, and can differentiate to the mesoderm lineage, such as adipocytes, bone cells, chondrocytes, etc., and can also differentiate to other germ layer lineage cells, such as epidermal cells \ vascular endothelium Cell Differentiation. [0003] The mesenchymal stem cell culture system contains a variety of active ingredients, such as: stem cell growth factor (SCF), fibroblast growth factor (FGF), endothelial cell growth factor (VEGF), epidermal growth factor (EGF), cell Colony-stimulating factor, interleukin (IL), collagen, hyaluronic acid a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/08A61P27/02A61K31/728
CPCA61K35/28A61K9/0048A61K9/08A61K31/728A61K2300/00
Inventor 秦臻鲁振宇韩洪起张冰晶熊亮高婧彧徐悦闻婧黄文敬
Owner TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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