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Photoelectric immunosensor for detecting TNF-alpha, and preparation method and application thereof

An immune sensor and photoelectric technology, applied in the field of biomedical detection, to achieve the effect of sufficient reaction sites, enhanced sensitivity, and easy modification

Active Publication Date: 2017-12-01
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current clinical analysis techniques such as enzyme-linked immunosorbent assay can only detect obvious changes, so that diseases are rarely detected early and intervened in treatment. Therefore, it is particularly important to find a more sensitive and specific method and platform for detecting trace antigens. important

Method used

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  • Photoelectric immunosensor for detecting TNF-alpha, and preparation method and application thereof
  • Photoelectric immunosensor for detecting TNF-alpha, and preparation method and application thereof
  • Photoelectric immunosensor for detecting TNF-alpha, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 GO-PTC-NH 2 preparation of

[0039] Weigh GO and PTC-NH with an analytical balance 2 0.5 mg each, carefully grind them in a precision mortar and mix thoroughly to form a fine powder, transfer to 2ml 1×PBS buffer solution for dissolution. After 30min of ultrasonic dispersion, the mixed solution was continuously stirred at room temperature for 20-24h to obtain 0.25mg / ml GO-PTC-NH 2 solution, the solution was purple, and kept in a refrigerator at 4°C in the dark until use.

Embodiment 2

[0040] The photoelectric activity detection of embodiment 2 signaling complex

[0041] (1) Instrument: Shanghai Chenhua electrochemical workstation (chi660e software), xenon lamp light source, multimeter

[0042] (2) Materials and reagents:

[0043] Electrode: ITO, the specification is 5cm×1cm

[0044] Electrolyte: 50mM Ascorbic Acid in Phosphate Buffer

[0045] Reagent: 0.25mg / ml GO solution (solvent 1×PBS buffer), 0.25mg / ml PTC-NH 2 Solution (solvent 1×PBS buffer), 0.25mg / ml GO-PTC-NH 2 Solution (solvent 1×PBS buffer)

[0046] (3) Method:

[0047] Electrode treatment: The ITO electrode was ultrasonically cleaned twice in absolute ethanol and 50% isopropanol aqueous solution, each time for 15 minutes. During the ultrasonic process, ensure that each surface of the entire electrode is in the treatment solution from beginning to end to prevent adsorption between electrodes. After complete treatment, take out the electrode and dry it in an oven at 37°C, then test the front...

Embodiment 3

[0053] The preparation method of embodiment 3 photoelectric immunosensor

[0054] (1) The signal layer is fixed

[0055] GO-PTC-NH 2 The complex was ultrasonicated for 30min at room temperature to make it a uniform dispersion, GO-PTC-NH 2 The concentration is 0.25mg / mL. Take 10 μL and carefully drop it on the front of the treated ITO electrode, and place it at room temperature for 24 hours to fix it. At this time, a thin and uniform light reddish-brown film can be observed on the surface of the electrode. After the reaction is completed, wash with deionized water to remove impurities on the surface of the electrode.

[0056] (2) Anchor recognition molecules

[0057] Take 10 μl of newly prepared 25 mg / ml glutaraldehyde (GA) solution as a cross-linking agent, by virtue of the aldehyde groups at both ends and the electrode surface material GO-PTC-NH obtained in step (1) respectively 2 The amino group of the amino group and the amino group of the antibody to be anchored form ...

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Abstract

The invention discloses a photoelectric immunosensor for detecting TNF-alpha, and a preparation method and application thereof. The surface of a photic substrate electrode is sequentially modified with a GO-PTC-NH2 solution and an anti-TNF-alpha solution, wherein the substrate electrode is a glassy carbon electrode or indium tin oxide semiconductor electrode. Compared with traditional ELISA and PCR methods or the like, the method of preparing the photoelectric immunosensor for detecting tumor markers from a GO-PTC-NH2 nanometer compound in the invention has the characteristics of simple operation, low technical requirements and rapid response; and an optoelectronic probe used in the invention overcomes the disadvantages of photocorrosion and the like of traditional metal materials, etc., has good stability, great load capacity and considerable functional groups, and is easy to modify.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and in particular relates to a photoelectric immune sensor for detecting TNF-α, a preparation method and application thereof. Background technique [0002] Tumor markers are molecular changes that can be detected in cancer patients' tumors or other body fluids such as blood and urine. Detection of changes in their content can be used as a diagnostic and prognostic indicator for the disease, for curative effect observation and monitoring of recurrence. Most of the current clinical analysis techniques such as enzyme-linked immunosorbent assay can only detect obvious changes, so that diseases are rarely detected early and intervened in treatment. Therefore, it is particularly important to find a more sensitive and specific method and platform for detecting trace antigens. important. Photoelectrochemical sensors have strong application prospects in the field of life analysis based on the conversi...

Claims

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Application Information

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IPC IPC(8): G01N27/403G01N33/53
CPCG01N27/403G01N33/53
Inventor 沈艳飞胡慧祯潘登张明明张袁健
Owner SOUTHEAST UNIV
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