A kind of DNA tissue sample preservation solution and its application

Abstract

The invention discloses a DNA tissue sample preservation solution and its application, belonging to the field of biotechnology. In the preservation solution, ammonium oxalate saturated solution, disodium edetate 0.05-2mM, trihydroxyaminomethane 10-50mM, polyethylene glycol 0.2-0.5% of diol octyl phenyl ether, 0.5-1.5% of sodium chloride, and the pH value is controlled between 7.2-8.1. The composition of the preservation solution is simple, safe, non-toxic, low-cost, stable in chemical properties, not volatile, and there is no need to replenish the preservation solution after one preservation; the preservation solution has no cutting effect on DNA, and large fragments of DNA can be extracted; in the later stage of DNA extraction No additional treatment is required, and the DNA extraction is simple; the high-salt environment of the preservation solution can dehydrate the tissue, and even if the tissue is preserved for a long time, it will not cause the tissue to "loose and crisp", so the tissue will not be reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the fixation and preservation of biological tissues, in particular to a DNA tissue sample preservation solution and its application. Background technique [0002] The long-term preservation of animal tissues is an important prerequisite for DNA sequencing, molecular genetics research, and forensic identification. The quality of animal tissue preservation directly affects the yield and quality of later DNA extraction. At present, the commonly used animal tissue preservation methods include cryogenic freezing, formaldehyde immersion, paraffin embedding and ethanol immersion, etc. Different preservation methods have their own characteristics, advantages and disadvantages. [0003] The cryogenic freezing method utilizes low temperature (-20°C ordinary refrigerator, -80°C ultra-low temperature freezer or -196°C liquid nitrogen) to inhibit the activity of DNase to preserve tissue DNA for a lo...

AI Technical Summary

Problems solved by technology

[0012] However, there are still many problems in the existing preservation methods, such as long-term preservation of the tissue resulting in "loose crisping" of the tissue and reducing the tissue, or although the DNA can be preserved, it is impossible to extract large fragments of DNA, or the preservation process requires Multiple replacement or replenishment of the preservation solution, and the preservation of the sample can be achieved, but it is unfavorable for subsequent DNA extraction, etc.

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