Method for controlling rice blast of rice

A technology of rice blast and rice, applied in the field of biological control of rice pests and diseases, can solve the problems of reducing control efficiency, polluting the environment, and easy variation, and achieves a good effect of biological control

Inactive Publication Date: 2017-12-29
安徽省中日农业环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex genetic background of the rice blast fungus, it is easy to mutate, and it is difficult for the disease-resistant varieties to keep up with the variation speed of the physiological races of the pathogenic bacteria, which often leads to the loss of resistance of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for controlling rice blast, comprising the following steps:

[0023] Step 1: Select healthy rice leaves, cut them into 5cm leaf segments, put them into a triangular flask filled with 75% ethanol, shake and wash twice, pour off the ethanol, add sterile water to soak for 30 minutes, and then make a gradient dilution to obtain a gradient dilution liquid;

[0024] Step 2: Prepare NA solid medium, use peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, agar 15.0g, glucose 2.5g, add distilled water to dissolve, adjust the volume to 1L, adjust the pH to 7±0.1, divide Sterilize at 121°C for 20 minutes after loading;

[0025] Step 3: Draw the gradient dilution and spread it on the NA solid medium. After drying, culture it upside down in a constant temperature incubator at 30°C for 48 hours, pick a single colony, and streak on the NA solid medium to obtain a single colony again. Bacterial colonies, the obtained single colonies are preserved in the slant of NA solid ...

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PUM

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Abstract

The invention discloses a method for controlling rice blast of rice, and relates to the technical field of biological control of rice pest and disease damage. The preparation method comprises the steps of gradient diluent preparation, NA solid culture medium preparation, bacillus separation, PDA culture medium preparation, spore solution preparation and rice colonization. Accordingly, bacilli obtained through separation can secrete an antibacterial material to inhibit pathogenic bacteria growth and induce a plant defence system to resist pathogenic bacteria invasion, supplied experimental strains have the significant inhibiting effect on germination of magaporthe grisea conidia, extension of germ tubes and formation of appressoria, the strain active substances have the high inhibiting effect on magaporthe grisea, and the magaporthe grisea mycelial growth inhibition rate reaches 80% or above; by means of silicon addition treatment, the rice seedling rice blast morbidity and disease index can be significantly lowered, the morbidity decreasing range can reach 30% or above, and the disease index is lowered by 40% or above. The resistance of the rice blast and the silicon content of different organs can be significantly increased through silicon treatment.

Description

technical field [0001] The invention relates to the technical field of biological control of rice diseases and insect pests, in particular to a method for preventing and treating rice blast. Background technique [0002] Rice is China's largest grain crop, and its output accounts for about 40% of China's grain output. The three major diseases of rice include rice blast, bacterial blight and rice sheath blight. Among them, rice blast is one of the most threatening diseases in rice production. It is caused by the fungus Pyrospora fungus and can occur throughout the rice development period. Due to the rapid prevalence of the disease, under suitable environmental conditions, it can cause a pandemic in a short period of time and bring huge losses to rice production. At present, in agriculture, disease-resistant breeding and chemical agents are mainly used to gradually control rice blast. However, due to the complex genetic background of Magnaporthe grisea, it is easy to mutate,...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01N63/00A01N59/00A01P3/00A01P21/00
CPCA01G13/00A01N59/00A01N63/00
Inventor 章云张贝尼
Owner 安徽省中日农业环保科技有限公司
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