Preparation method of antibacterial composite bacterial cellulose membrane

The technology of bacterial cellulose film and bacterial cellulose is applied in the field of preparation of antibacterial composite bacterial cellulose film, which can solve the problems of affecting the quality of wound healing and recovery, and cannot prevent wound infection, and achieves low cost and good antibacterial effect. Effect

Inactive Publication Date: 2017-12-29
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the prior art, the present invention provides a method for preparing an antibacterial composite bacterial cellulose film, which overcomes the problems in the prior art that bacterial cellulose cannot prevent wound infection and affects the healing and post-healing quality of the wound

Method used

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  • Preparation method of antibacterial composite bacterial cellulose membrane
  • Preparation method of antibacterial composite bacterial cellulose membrane
  • Preparation method of antibacterial composite bacterial cellulose membrane

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: fermentation and processing of bacterial cellulose film

[0028] 1) culture medium

[0029] Seed medium: 10g of glucose, 10g of yeast extract, 1000mL of distilled water, sterilized at 115°C for 20min, and added 3% absolute ethanol (V / V) when cooling to about 50°C.

[0030] Fermentation medium: glucose 20g, yeast extract 10g, peptone 7g, disodium hydrogen phosphate 7.5g, citric acid 0.5g, distilled water 1000mL, sterilized at 115°C for 20min.

[0031] 2) Acetobacter xylinum cellulose fermentation

[0032] Pick Acetobacter xylinus (Gluconacetobacter xylinus) TJU-S8 from the slant and inoculate it in 100 mL of seed medium, culture at 30° C. with shaking at 120 r / min for 18 hours, and prepare seed liquid.

[0033] The seed solution was inserted into the fermentation medium at a ratio of 10%, and cultured statically at 30° C. for 7 days to obtain the bacterial cellulose membrane.

[0034] 3) Extraction of bacterial cellulose

[0035] The bacterial cellulose...

Embodiment 2

[0036] Embodiment 2: bacterial cellulose composite film preparation

[0037] The dried bacterial cellulose membrane was cut into discs with a diameter of 10 mm. Place in 0.6% chitosan / carboxymethyl chitosan-citric acid aqueous solution, stir and incubate at room temperature for 24 hours, take it out for vacuum freeze-drying for 12 hours, and store in a dehumidifier. In this example, a bacterial cellulose composite membrane with an ultrafine network structure and evenly distributed pores was obtained.

Embodiment 3

[0038] Embodiment 3: Bacterial cellulose composite membrane structural characteristic analysis

[0039] 1) Scanning electron microscope analysis

[0040] Cut the treated bacterial cellulose into 5mm 2 The small squares on the left and right are sprayed with gold coating on the ion gold plating instrument. The microstructure of the coated samples was observed with a scanning electron microscope.

[0041] 2) Fourier transform infrared spectroscopic analysis

[0042] The infrared absorption spectrum of bacterial cellulose was measured by Fourier transform infrared spectrometer. Mix the dried bacterial cellulose sample with KBr and grind it, and press it into thin slices for detection, the range is 4000-500cm -1 .

[0043] 2) X-ray diffraction analysis

[0044] Use an X-ray diffractometer to fix the dried bacterial cellulose on the frame, keep it flat, copper target, test voltage 40KV, test current 15mA, rate 10° / min, cloth width 0.02°, 2θ 10-80 ° large-scale scanning; then...

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Abstract

The invention discloses a preparation method of an antibacterial composite bacterial cellulose membrane. The preparation method comprises the following steps: by adopting gluconacetobacter xylinus TJU-S8 as a starting strain, standing for culture to generate bacterial cellulose, and by adopting the bacterial cellulose as a material and combining with chitosan / carboxymethyl chitosan, preparing the composite bacterial cellulose membrane with an antibacterial property and compact fiber structure. The preparation method disclosed by the invention has the advantages that the defect of no antibacterial property of the bacterial cellulose is solved, a preparation process of an antibacterial composite membrane capable of inhibiting growth of escherichia coli and staphylococcus aureus is formed, the antibacterial activity of the bacterial cellulose composite membrane is improved, and a new path is provided for expanding the application field of bacterial cellulose. The antibacterial composite bacterial cellulose membrane obtained by the method is economic and environment-friendly, and is good in antibacterial effect.

Description

technical field [0001] The invention relates to a preparation method of an antibacterial composite bacterial cellulose film. Background technique [0002] Bacterial Cellulose (BC for short) is a polysaccharide composed of D-glucopyranose connected by β-1,4 glycosidic bonds, also known as β-1,4 glucan. At present, there are nine genera of microorganisms capable of synthesizing bacterial cellulose: Acetobacter, Azotobacter, Achromobacter, Aerobacter, and Sarcina , Agrobacterium (Agrobacterium), Alcaligenes (Alcaligcncs), Rhizobium (Rhizobium), Pseudomonas (Pseudomonas). Among them, Gluconacetobacter xylinus is currently the most commonly studied in experiments and production, and it is the genus most widely used in the production of bacterial cellulose. Bacterial cellulose has been widely used in various fields due to its good water holding capacity, mechanical strength, biological adaptability, and degradability. Related products in the commercial field include tires, audi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08J7/00C08J5/18C08L1/02
CPCC08J5/18C08J7/00C08J9/26C08J2201/0504C08J2301/02
Inventor 周志江杜仁鹏袁望舒殷楠郭尧婷赵芳坤韩烨
Owner TIANJIN UNIV
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