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A method for simultaneously detecting multivitamin content in nutrient soft capsules

A vitamin and soft capsule technology, applied in the field of drug analysis, can solve the problems of simultaneous detection of multiple components, large consumption of reagents, and high detection costs, and achieves protection from impurities, short detection time, and accurate and stable results. Effect

Active Publication Date: 2020-04-17
FERGUSON WUHAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, this method can only realize the detection of one or two components, resulting in a large consumption of reagents in the whole process, a long detection cycle, high detection costs, and the accuracy and sensitivity of existing detection methods are relatively poor.
[0003] CN 1582756A discloses a nutrient soft capsule for pregnant women, which is composed of vitamin C, nicotinamide, vitamin E, pantothenic acid, vitamin B6, vitamin B1, vitamin B2, β-carotene, folic acid, vitamin D2, vitamin B12, anhydrous hydrogen phosphate Calcium, heavy magnesium oxide and other vitamins and minerals. At present, the vitamin components can only be detected in multiple times. There is no literature report on the simultaneous detection of multiple components.

Method used

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  • A method for simultaneously detecting multivitamin content in nutrient soft capsules
  • A method for simultaneously detecting multivitamin content in nutrient soft capsules
  • A method for simultaneously detecting multivitamin content in nutrient soft capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The selection of embodiment 1 sample solution preparation method

[0033] 1. Take 1g of the contents of the capsule, put it in a 50ml volumetric flask, add an appropriate amount of mobile phase, ultrasonicate at 45°C for 30 minutes, cool to room temperature, dilute to the mark with the mobile phase, shake well, take 10ml and centrifuge, take the supernatant with a 0.45μm Membrane filtration, discard the initial filtrate, and take the subsequent filtrate as the test solution.

[0034] 2. Take 1g of the contents of the capsule, put it in a 50ml volumetric flask, add an appropriate amount of water, ultrasonicate at 45°C for 30min, cool to room temperature, dilute with water to the mark, shake well, take 10ml and centrifuge, and take the supernatant through a 0.45μm filter membrane Filter, discard the initial filtrate, and take the subsequent filtrate as the test solution.

[0035] 3. Take 1g of the contents of the capsule, put it in a 50ml volumetric flask, add an appropr...

Embodiment 2

[0045] The selection of embodiment 2 detection wavelength

[0046] Preparation of sample solution: Take 1g of the contents of the capsule, put it in a 50ml volumetric flask, add an appropriate amount of mobile phase, ultrasonicate at 45°C for 30min, cool to room temperature, dilute to the mark with mobile phase, shake well, take 10ml of centrifuge, and take the supernatant for use Filter through a 0.45 μm filter membrane, discard the initial filtrate, and take the subsequent filtrate as the test solution.

[0047] Detection wavelength: 260nm, 290nm

[0048] Other conditions are identical with embodiment 1.

[0049] Result: from figure 1 ﹑ Figure 4 It can be seen that when the wavelength is 290nm, vitamin B1 (17.652min) and vitamin B1 (18.539min) cannot be detected effectively, and an extra 3.304min interferes with the determination of vitamin C; when the wavelength is 260nm, vitamin C (2.830min), smoke Amide (4.262min), vitamin B6 (6.962min), folic acid (14.438min), vitam...

Embodiment 3

[0050] The selection of embodiment 3 mobile phases

[0051] (1) A is a buffered saline solution containing 50mM disodium hydrogen phosphate, adjusted to pH 3 with phosphoric acid; B is acetonitrile

[0052] (2) A is a buffered saline solution containing 2mM sodium heptanesulfonate, adjusted to pH 3 with phosphoric acid; B is acetonitrile

[0053] (3) A is a buffered saline solution containing 2mM sodium heptanesulfonate and 50mM disodium hydrogen phosphate, and the pH is adjusted to 5.0 with phosphoric acid; B is acetonitrile

[0054] (4) A is a buffered saline solution containing 2mM sodium heptanesulfonate and 50mM disodium hydrogen phosphate, and the pH is adjusted to 3.0 with phosphoric acid; B is acetonitrile

[0055] The mobile phase volume ratio is 90:10;

[0056] Other conditions are identical with embodiment 1.

[0057] Result: by figure 1 , Figure 5-7 It can be seen that Figure 5 Incomplete separation of each chromatographic peak, and severe tailing; Figure 6...

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Abstract

The invention discloses a method for detecting content of multivitamins in a soft nutrient capsule simultaneously. The method comprises steps as follows: a to-be-detected sample is extracted and detected by an HPLC (high performance liquid chromatograph), the HPLC is provided with an ultraviolet detector, set detection wavelength of the ultraviolet detector is 250-280 nm, a chromatographic columnof the HPLC is C18 chromatographic column, a moving phase is a mixed solution of a buffer solution and acetonitrile, constant-speed elution is performed, and the column temperature is 25-35 DEG C. Compared with the prior art, content determination of six vitamins can be executed once, a detection result is high in precision and is more accurate and more stable, and the operating process is safer and simpler.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for simultaneously detecting the contents of multiple vitamins in nutrient soft capsules. Background technique [0002] With the development of society and the improvement of nutritional needs, more and more products containing multivitamins appear on the market. Excessive supplementation of vitamins is also harmful to the body. Therefore, it is necessary to monitor the vitamin content in the products, which requires a A rapid determination method, high performance liquid chromatography is a feasible method for the determination of vitamin content. However, at present, this method can only detect one or two components, resulting in a large consumption of reagents in the whole process, a long detection cycle, high detection costs, and the accuracy and sensitivity of the existing detection methods are relatively poor. [0003] CN 1582756A discloses a nutrient soft capsule for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/74
Inventor 洪继兵赵小伟欧阳康乐
Owner FERGUSON WUHAN BIOTECH
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