Mixed strain fermented natto chewable tablets and preparation method thereof
A technology of mixed strains and chewable tablets, which is applied in the field of mixed strain fermented natto chewable tablets and its preparation, can solve the problems of difficult popularization and application by the whole people, no taste of natto, and high production cost, so as to improve bioavailability, shorten production cycle, Quickly dissolves and absorbs
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Embodiment 1
[0047] A mixed strain fermented natto chewable tablet of the present invention consists of the following formula in terms of mass percentage:
[0048] Mixed strain fermented natto 52%, magnesium stearate 1%, microcrystalline cellulose 2%, maltodextrin 10%, silicon dioxide 1%, milk powder 18%, citric acid 1%, white sugar 15%.
[0049] The preparation method of mixed strain fermented natto chewable tablet of the present invention comprises the steps:
[0050] (1) Pretreatment: weigh high-quality soybeans with similar shapes and sizes, wash and soak them for 15 hours; sterilize soybeans at 121°C for 15 minutes in an autoclave, cool to room temperature, and set aside;
[0051] (2) Preparation of seed solution:
[0052] Bacillus natto was inoculated into LB liquid medium, and aerobically cultured at 35°C for 18 hours; Pediococcus pentosaceus was inoculated into MRS liquid medium, and anaerobically cultured at 30°C for 18h;
[0053] (3) Preparation of mixed strain fermented natto: i...
Embodiment 2
[0056] A mixed strain fermented natto chewable tablet of the present invention consists of the following formula in terms of mass percentage:
[0057] Mixed strain fermented natto 50.4%, microcrystalline cellulose 1.2%, isomaltooligosaccharide 1%, silicon dioxide 1.2%, carrageenan 2%, milk powder 26.4%, citric acid 2%, white sugar 15.8%.
[0058] The preparation method of mixed strain fermented natto chewable tablet of the present invention comprises the steps:
[0059] (1) Pretreatment: weigh high-quality black beans with similar shapes and sizes, wash and soak for 16 hours; sterilize the black beans in an autoclave at 121°C for 30 minutes, cool to room temperature, and set aside;
[0060] (2) Preparation of seed liquid: Bacillus natto was inoculated into LB medium by 2% inoculum amount, cultivated in a shaker at 36° C. 150 r / min for 19 hours, and Bifidobacterium was inoculated into MRS medium by 2% inoculum amount cultured at 32°C for 19 hours; the fungus was inoculated on ...
Embodiment 3
[0064] A mixed strain fermented natto chewable tablet of the present invention consists of the following formula in terms of mass percentage:
[0065] Mixed strain fermented natto 51.5%, magnesium stearate 0.7%, microcrystalline cellulose 1.4%, maltodextrin 7%, carrageenan 3%, milk powder 18.8%, citric acid 4.6%, white sugar 13%.
[0066] The preparation method of mixed strain fermented natto chewable tablet of the present invention comprises the steps:
[0067] (1) Pretreatment: weigh high-quality soybeans with similar shapes and sizes, wash and soak them for 17 hours; sterilize soybeans in an autoclave at 121°C for 25 minutes, cool to room temperature, and set aside;
[0068] (2) Preparation of seed liquid: Bacillus natto was inoculated into LB medium according to 2% inoculum amount, cultivated in a shaker at 37° C. 150 r / min for 20 h, and Lactobacillus bulgaricus was inoculated into MRS medium according to 2% inoculum amount. Cultivate at 35° C. for 20 h; inoculate Aspergi...
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