Method for preparing high-efficiency straw fermentation agent

Abstract

The invention discloses a method for preparing a high-efficiency straw fermentation agent and belongs to the technical field of preparation of fermentation agents. Two kinds of cellulomonas used in the invention refer to a category of bacteria capable of decomposing cellulose and partial xylogen, belong to facultative anaerobes, can be released after autocytolysis and have high degradability on the cellulose under anaerobic conditions. A synergistic effect is achieved between a bacterium decomposition effect and a decomposition effect of enzymes produced in cells. The third added bacillus subtilis can produce various enzymes needed by animals, the bacterium culture medium is subjected to high-temperature or high-pressure steam treatment, sterilized and disinfected, and subjected to viablebacteria inoculation, the inoculated bacillus subtilis produces lots of spores in the reproductive process, many bacteria die during fermentation due to poor heat resistance, the bacterium death rateis reduced, the viable bacteria reach a higher concentration, and the influence on bacterial reproduction subjected to acid stress is reduced. Therefore, the aim of improving the viable bacteria countis achieved.

Description

technical field

[0001] The invention discloses a preparation method of a high-efficiency straw starter, which belongs to the technical field of starter preparation. Background technique

[0002] Starter refers to cultures of bacteria and other microorganisms used in the production of yogurt, kefir, cream, cheese, natto and other fermented products. Many microorganisms can be used as leavening agents, including some bacteria of the genera Lactobacillus, Micrococcus, Staphylococcus, Pediococcus, Streptococcus, Lactococcus and Bacillus, as well as yeasts, molds and actinomycetes Some fungi can be used as starters.

[0003] Straw starter: under the action of high-efficiency organisms (factors of various decomposing enzymes, a variety of microbial live bacteria), the crude fiber (cellulose, hemicellulose), lignin, xylan long molecular chain, woody The ester bond of the compound is enzymatically hydrolyzed, and the high-molecular carbohydrates that animals cannot absorb are conv...

Examples

example 1

[0025] In terms of parts by weight, 20 parts of corn stalks were pulverized in a pulverizer, passed through a 100-mesh sieve to obtain corn stalk powder, and 20 parts of agar, 2 parts of yeast powder, 5 parts of wheat bran, and 20 parts of corn stalk powder were weighed and placed in In a beaker, heat it in a water bath to 90°C, and keep it warm for 10 minutes to obtain a slant preservation medium; in parts by weight, put 10 parts of peptone, 8 parts of glucose, 10 parts of yeast extract, and 50 parts of 0.9% saline in the culture medium. In a dish, stir and mix for 3 minutes to obtain a liquid activated medium, put the liquid activated medium in a high-pressure steam with a temperature of 120°C and an air pressure of 0.14MPa for 15 minutes to obtain a sterilized liquid activated medium; dinitrogen The bacterial strains of Cellulomonas, Cellulomonas luteum and Bacillus subtilis are mixed according to the mass ratio of 1:1:2 to obtain the mixed strains, and a mixture of 5% of th...

example 2

[0027] In terms of parts by weight, 25 parts of corn stalks were pulverized in a pulverizer, passed through a 100-mesh sieve to obtain corn stalk powder, weighed 25 parts of agar, 3 parts of yeast powder, 6 parts of wheat bran, and 22 parts of corn stalk powder In a beaker, heat it in a water bath to 95°C, and keep it warm for 12 minutes to obtain a slant preservation medium; in parts by weight, put 11 parts of peptone, 9 parts of glucose, 12 parts of yeast extract, and 52 parts of 0.9% normal saline in the culture medium. In a dish, stir and mix for 4 minutes to obtain a liquid activated medium, put the liquid activated medium in a high-pressure steam with a temperature of 122°C and an air pressure of 0.15 MPa for 17 minutes to obtain a sterilized liquid activated medium; dinitrogen Cellulomonas, Cellulomonas luteum and Bacillus subtilis are mixed according to the mass ratio of 1:1:2 to obtain the mixed strains, and the mixture of 6% of the mass of the slant preservation medium ...

example 3

[0029] In terms of parts by weight, 30 parts of corn stalks were pulverized in a grinder, passed through a 100-mesh sieve to obtain corn stalk powder, 30 parts of agar, 4 parts of yeast powder, 7 parts of wheat bran, and 25 parts of corn stalk powder were weighed and placed in In a beaker, heat it in a water bath to 100°C and keep it warm for 15 minutes to obtain a slant preservation medium; in parts by weight, put 12 parts of peptone, 10 parts of glucose, 15 parts of yeast extract, and 55 parts of 0.9% normal saline in the culture medium. In a dish, stir and mix for 5 minutes to obtain a liquid activated medium, put the liquid activated medium in high-pressure steam with a temperature of 125°C and an air pressure of 0.16MPa for 30 minutes to obtain a sterilized liquid activated medium; dinitrogen The strains of Cellulomonas, Cellulomonas flavinum and Bacillus subtilis are mixed according to the mass ratio of 1:1:2 to obtain the mixed strains, and a mixture of 8% of the mass of...

technical field

[0001] The invention discloses a preparation method of a high-efficiency straw starter, which belongs to the technical field of starter preparation. Background technique

[0002] Starter refers to cultures of bacteria and other microorganisms used in the production of yogurt, kefir, cream, cheese, natto and other fermented products. Many microorganisms can be used as leavening agents, including some bacteria of the genera Lactobacillus, Micrococcus, Staphylococcus, Pediococcus, Streptococcus, Lactococcus and Bacillus, as well as yeasts, molds and actinomycetes Some fungi can be used as starters.

[0003] Straw starter: under the action of high-efficiency organisms (factors of various decomposing enzymes, a variety of microbial live bacteria), the crude fiber (cellulose, hemicellulose), lignin, xylan long molecular chain, woody The ester bond of the compound is enzymatically hydrolyzed, and the high-molecular carbohydrates that animals cannot absorb are conv...

Examples

example 1

[0025] In terms of parts by weight, 20 parts of corn stalks were pulverized in a pulverizer, passed through a 100-mesh sieve to obtain corn stalk powder, and 20 parts of agar, 2 parts of yeast powder, 5 parts of wheat bran, and 20 parts of corn stalk powder were weighed and placed in In a beaker, heat it in a water bath to 90°C, and keep it warm for 10 minutes to obtain a slant preservation medium; in parts by weight, put 10 parts of peptone, 8 parts of glucose, 10 parts of yeast extract, and 50 parts of 0.9% saline in the culture medium. In a dish, stir and mix for 3 minutes to obtain a liquid activated medium, put the liquid activated medium in a high-pressure steam with a temperature of 120°C and an air pressure of 0.14MPa for 15 minutes to obtain a sterilized liquid activated medium; dinitrogen The bacterial strains of Cellulomonas, Cellulomonas luteum and Bacillus subtilis are mixed according to the mass ratio of 1:1:2 to obtain the mixed strains, and a mixture of 5% of th...

example 2

[0027] In terms of parts by weight, 25 parts of corn stalks were pulverized in a pulverizer, passed through a 100-mesh sieve to obtain corn stalk powder, weighed 25 parts of agar, 3 parts of yeast powder, 6 parts of wheat bran, and 22 parts of corn stalk powder In a beaker, heat it in a water bath to 95°C, and keep it warm for 12 minutes to obtain a slant preservation medium; in parts by weight, put 11 parts of peptone, 9 parts of glucose, 12 parts of yeast extract, and 52 parts of 0.9% normal saline in the culture medium. In a dish, stir and mix for 4 minutes to obtain a liquid activated medium, put the liquid activated medium in a high-pressure steam with a temperature of 122°C and an air pressure of 0.15 MPa for 17 minutes to obtain a sterilized liquid activated medium; dinitrogen Cellulomonas, Cellulomonas luteum and Bacillus subtilis are mixed according to the mass ratio of 1:1:2 to obtain the mixed strains, and the mixture of 6% of the mass of the slant preservation medium ...

example 3

[0029] In terms of parts by weight, 30 parts of corn stalks were pulverized in a grinder, passed through a 100-mesh sieve to obtain corn stalk powder, 30 parts of agar, 4 parts of yeast powder, 7 parts of wheat bran, and 25 parts of corn stalk powder were weighed and placed in In a beaker, heat it in a water bath to 100°C and keep it warm for 15 minutes to obtain a slant preservation medium; in parts by weight, put 12 parts of peptone, 10 parts of glucose, 15 parts of yeast extract, and 55 parts of 0.9% normal saline in the culture medium. In a dish, stir and mix for 5 minutes to obtain a liquid activated medium, put the liquid activated medium in high-pressure steam with a temperature of 125°C and an air pressure of 0.16MPa for 30 minutes to obtain a sterilized liquid activated medium; dinitrogen The strains of Cellulomonas, Cellulomonas flavinum and Bacillus subtilis are mixed according to the mass ratio of 1:1:2 to obtain the mixed strains, and a mixture of 8% of the mass of...