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Rhodopseudomonas palustris RP1 and application thereof

A swamp rhodopseudomonas and strain technology, applied in the field of environmental microorganisms, can solve the problems of many types of activated sludge microorganisms and unclear composition, etc.

Active Publication Date: 2018-02-09
YUNNAN WAN KUI BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The soil of cultivated plants and the sludge of paddy fields, swamps, lakes, and rivers and seas are rich in microorganisms, some of which have a mechanism of formaldehyde metabolism, so many studies have tried to use potted plant soil or sludge from water to assemble bioreactors. The research results It is confirmed that these bioreactors are very effective in removing and degrading various organic pollutants, but there are many types of microorganisms in activated sludge, the composition is not clear, and some pathogenic bacteria may be contained

Method used

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  • Rhodopseudomonas palustris RP1 and application thereof
  • Rhodopseudomonas palustris RP1 and application thereof
  • Rhodopseudomonas palustris RP1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Screening, identification and detection of embodiment 1 bacterial strain

[0019] Rhodopseudomonas palustris of the present invention is a kind of photosynthetic bacterium that is obtained by using formaldehyde to domesticate microorganisms in sewage ditch muddy water as material, and obtains with the inorganic salt culture medium of photosynthetic bacteria, and its specific screening steps are:

[0020] 1. Domestication of activated sludge

[0021] Collect river sludge water, filter it with gauze to remove large particles, add formaldehyde to the filtrate to domesticate the microorganisms in the sludge water, the concentration of formaldehyde increases from the initial 0.5mM to the final 10mM day by day, and the treatment lasts for 20 days .

[0022] 2. Isolation of Photosynthetic Bacteria

[0023] After the acclimatization treatment, an appropriate amount of sludge aqueous solution was inoculated into 500 mL photosynthetic bacteria culture medium (1L culture medium ...

Embodiment 2

[0027] Example 2 Analysis of RP1's resistance to formaldehyde and efficiency of absorbing formaldehyde

[0028] Patent No. 201710038574.1 discloses a strain of Rhodopseudomonas palustris ( Rhodopseudanonas palustris ) DC-2, whose preservation number in the China Center for Type Culture Collection is CCTCC NO: M2016677, this strain has the ability to remove formaldehyde, and can be used to treat formaldehyde-contaminated sewage. In order to compare the physiological and biochemical characteristics of the strain, RP1 and DC-2 were inserted into an anaerobic tube containing 25 ml of photosynthetic bacteria liquid medium, and 4 mM and 6 mM formaldehyde were added when the OD375 was 0.5-0.6, 23 Cultivate under light at -25°C, measure the OD375 of the bacteria at 1, 2, 3, 4, 5, 6, 7, and 8 days after inoculation, and observe the growth of the bacteria. The results showed that the OD375 of RP1 and DC-2 had no significant difference in the 1-2 days, showing a gradually increasing tren...

Embodiment 3

[0031] 1. Comparison of RP1 and DC-2 metabolic conversion mechanism of formaldehyde

[0032] Collect 2 g of DC-2 and RP1 bacterial cells by centrifugation, and use 4 mM H 13 CHO solution (5 ml, containing 5 mM NaHCO 3 , 0.1% MES (2-N-Morpholino ethanesulfonic acid, W / V) was treated for 24 h under 60W incandescent light, and the untreated sample was used as the background control. After the treatment, the bacteria were collected by centrifugation, suspended in 3ml of sterile water, and ultrasonically crushed to extract soluble metabolites. 13 C-NMR nuclear magnetic resonance analysis H 13 CHO metabolic profile ( Figure 4 ). Passed and Known Compounds 13 The C-NMR spectra were compared to deduce the assignment of the resonance peaks. The target resonance peak was integrated with formamide as an internal reference, and the relative content of each metabolite in different samples was calculated. The results indicated that DC-2 and RP1 had different mechanism of formaldehyd...

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Abstract

The invention discloses a rhodopseudomonas palustris strain RP1, which is preserved in China Center for Type Culture Collection with preservation number of CCTCC: M2017324. The rhodopseudomonas palustris is a photosynthetic bacterium which is obtained by conducting isolation on microorganisms in formaldehyde domesticated bilge muddy water by virtue of an inorganic salt medium of photosynthetic bacteria; the rhodopseudomonas palustris belongs to gram negative bacteria; a bacterium solution of the rhodopseudomonas palustris is represented in red; and carotenoids and bacteriochlorophyll a exist in bacterium cells. The rhodopseudomonas palustris, when exposed to illumination, can achieve continuous growth in a liquid inorganic salt medium which contains 4.6mM of formaldehyde, and a relativelygood removal rate can be achieved on the formaldehyde added to the medium. The formaldehyde can be assimilated into various amino acids such as glutamic acid, glycine, alanine, phosphoenolpyruvic acid, acetic acid, ethanol and the like as well as organic acids; the formaldehyde can be assimilated into the various amino acids and organic acids on the basis of a major mechanism of formaldehyde detoxification; and through the mechanism, the RP1 photosynthetic bacterium shows relatively strong resistance and absorbing capacity on the formaldehyde.

Description

technical field [0001] The invention belongs to the field of environmental microbes, and in particular relates to a Rhodopseudomonas syringae which has a special formaldehyde metabolism pathway and can purify formaldehyde pollution. Background technique [0002] Formaldehyde is a colorless gas with a strong pungent smell. Formaldehyde is used in many industrial production processes, including chemical synthesis, industrial manufacturing, and pharmaceutical synthesis. Therefore, a large amount of waste water containing formaldehyde will be produced in these industrial production processes. These waste water are directly Emissions not only do not meet environmental protection requirements, but also have hazards to animals, plants and human health. On the other hand, with the continuous improvement of people's quality of life, decoration has become a part of modern people's life, and the materials used for decoration, such as plywood, blockboard, medium density fiberboard and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/01
CPCB01D53/72B01D53/84B01D2257/70C12N1/205C12R2001/01Y02A50/20
Inventor 李昆志陈丽梅雷玉珠冯永
Owner YUNNAN WAN KUI BIOLOGICAL SCI & TECH CO LTD