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Penicillium oxalicum as well as preparation method and application thereof

A technology of Penicillium oxalate and Penicillium oxalate, applied in the field of microorganisms, can solve problems such as unreported prevention research and application, and achieve the effects of less pollution, wide antibacterial spectrum and less dosage

Inactive Publication Date: 2018-02-16
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the control research and application of Penicillium oxalicum on kiwifruit canker, bacterial perforator, apple ring spot and peach brown rot, which are the main diseases of fruits and vegetables.
Pine pollen is the dry pollen of Pinus massoniana Lamb., Pinus tabuliformis Carr. or several plants of the same genus. It is rich in protein, lipid, amino acid, various trace elements, vitamins, enzymes, pigments and antibiotics, etc. , using pine pollen as a nutrient to cultivate Penicillium oxalicum to enhance the bactericidal ability of the study has not been reported

Method used

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  • Penicillium oxalicum as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0045] The recheck identification of embodiment 1 Penicillium oxalicum

[0046] 1. Morphological identification

[0047] The colonies were inoculated on carboxymethylcellulose sodium (CMC-Na) differential solid medium for colony morphology observation and microscopic examination. After the strain was cultured on carboxymethylcellulose sodium (CMC-Na) differential solid medium, the colonies were grayish green at first, evenly distributed on the surface of the medium, in the shape of rings, the edges were colorless and translucent, and the back of the colonies was turquoise or dark green. Under the microscope, it can be seen that the hyphae are colorless, and the conidiophores are scattered, growing vertically from the mycelium, the conidiophores are long columnar, and the lower part of the conidiophores is not branched; there is a diaphragm, and the tops of the conidiophores are arranged in broom-like Interbranches, the top layer of interbranches is a small stalk, the small s...

Embodiment 2

[0067] The medium screening of embodiment 2 Penicillium oxalicum

[0068] Penicillium oxalicum uses an 8mm hole puncher under aseptic operation conditions, selects a single and neat place to punch holes, and obtains 5 bacteria cakes of the same size, which are added to 100mL flavored PDA liquid medium, and 180r / m at 25°C. Cultivate on a shaker for 7 days, transfer it to the next batch of flavored PDA liquid medium at a 10% inoculum size, continue to cultivate for 7 days, and then transfer it to the next batch of flavored PDA at a 10% inoculum size In the culture medium, continue to cultivate for 7 days. After transferring 5 times according to this method, take 0.1 mL of the culture solution and transfer it to a flavored PDA solid culture dish, spread the plate, and place it in a constant temperature incubator at 25°C for 48 hours. Select a single colony Neat single colonies were inoculated on flavored PDA solid medium.

[0069] At the same time, repeat the above operation wit...

Embodiment 3

[0070] Embodiment 3: Penicillium oxalicum and multiple phytopathogenic bacterium plate confrontation test (common PDA medium)

[0071] Prepare pathogenic bacteria cakes and Penicillium oxalicum cakes with a diameter of 8 mm, and place the pathogenic bacteria cakes (8 mm) and antagonistic bacteria cakes (8 mm) equidistantly on both sides of the center of the ordinary PDA medium medium to form a straight line; no antagonistic bacteria are used as blank controls . Each treatment was repeated 5 times, and cultured in a constant temperature incubator at 25°C. After 5 days, the size of the inhibition zone was observed, and the diameter of the colony was measured by the cross method. The results are shown in Table 1. The results showed that the antagonism of Penicillium oxalicum on peach brown rot, apple ring rot, potato dry rot and bacterial perforator was between 35-52%, which was significantly lower than the inhibition rate of medium plus pine pollen.

[0072] Table 1 The confro...

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Abstract

The invention provides a preparation method of penicillium oxalicum. The preparation method comprises the steps: seed culture, amplification culture, liquid membrane extraction and bactericide preparation. The penicillium oxalicum is capable of preventing and controlling various fruit and vegetable diseases by single strain, wide in antibacterial spectrum and capable of effectively reducing the preventing and controlling costs; and the bacterium inhibiting rate for various fruit and vegetable diseases such as kiwifruit bacterial canker, bacterial leaf shot hole, apple ring rot, potato dry rotand peach brown rot reaches up to 90% or above.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a penicillium oxalic acid fungicide, a preparation method thereof and an application in preventing and treating main diseases of fruits and vegetables. Background technique [0002] Plant pathogenic fungi such as peach brown rot, bacterial perforator, kiwifruit canker, and apple ring spot do serious damage to crops and are difficult to control. At present, chemical pesticides are an effective means to prevent and treat plant diseases, and play a huge role in ensuring high and stable crop yields. However, the long-term unreasonable use of large quantities of traditional chemical pesticides caused residues, resistance and re-emergence, which has aroused widespread concern in the society. It is more and more urgent to seek and develop new pesticides that are harmful to human health and ecological safety. [0003] Liquid membrane extraction has two technical characteristics of solvent...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P1/00A01P3/00C12N1/14C12R1/80
CPCA01N63/30C12N1/14
Inventor 葛喜珍李朋钰杨涛李映田平芳
Owner BEIJING UNION UNIVERSITY
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