Conductive collagen heart patch preparation method

A collagen and original protein technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of poor toughness and electronic conduction, achieve easy biodegradability, solve toughness and poor electronic conduction, and enhance pH responsiveness Effect

Inactive Publication Date: 2018-02-27
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems in the prior art, the present invention provides a method for preparing a conductive collagen heart patch. The heart patch prepared by the method has high mechanical strength and strong electronic conductivity, thereby solving the problem of the common heart repair material in the process of using. Limitations such as toughness and poor electronic conductivity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method of a conductive collagen heart patch, the steps are as follows:

[0034] (1) Preparation of carbon nanotube dispersion: add 0.01g single-walled carbon nanotubes to 20mL ultrapure water to obtain 0.5g / L carbon nanotube ultrapure aqueous solution; 0.2g chitosan (Mw=200,000) Add the chitosan solution that obtains 2% (weight / volume) in 10mL acetic acid solution; Then get 10mL carbon nanotube ultrapure aqueous solution, add 10mL 2% (weight / volume) chitosan solution wherein, this mixture is in Ultrasonic treatment for 2 hours at 40% intensity with 400W sonic horn.

[0035] (2) Collagen cross-linking:

[0036] Add 0.5g collagen to 100mL acetic acid with a concentration of 0.1mol / L, stir at room temperature for 3 hours to obtain a 0.5% (W / V) collagen solution; mix 20mL of the above carbon nanotube dispersion with 100mL collagen solution and 10mL 2mol / L NaOH Mix the solution well; then add 10mL 0.5mol / L 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydroch...

Embodiment 2

[0044] A preparation method of a conductive collagen heart patch, the steps are as follows:

[0045](1) Preparation of carbon nanotube dispersion: add 0.04g single-walled carbon nanotubes to 40mL ultrapure water to obtain 1.0g / L carbon nanotube ultrapure aqueous solution; 0.4g chitosan (Mw=250,000) Add in 20mL acetic acid solution and obtain 2% (weight / volume) chitosan solution; Then get 20mL carbon nanotube ultrapure aqueous solution, add 20mL 2% (weight / volume) chitosan solution wherein. The mixture was sonicated for 4 hours with a 400W sonic horn at 40% intensity.

[0046] (2) Collagen cross-linking:

[0047] Add 1.0 g of collagen to 100 mL of 0.2 mol / L acetic acid, and stir at room temperature for 4 hours to obtain a 1.0% (W / V) collagen solution. Mix 40mL of the above-mentioned carbon nanotube dispersion with 100mL of collagen solution and 20mL of 2-(N-morpholino)ethanesulfonic acid (MES) buffer solution whose pH is 5.5; then mix 15mL of 0.5mol / L 1-( 3-Dimethylaminoprop...

Embodiment 3

[0051] A preparation method of a conductive collagen heart patch, the steps are as follows:

[0052] (1) Preparation of carbon nanotube dispersion: add 0.01g single-walled carbon nanotubes to 50mL ultrapure water to obtain 0.2g / L carbon nanotube ultrapure aqueous solution; 0.1g chitosan (Mw=200,000) Add in 10mL acetic acid solution and obtain 1% (weight / volume) chitosan solution; Then get 10mL carbon nanotube ultrapure aqueous solution, add 10mL 1% (weight / volume) chitosan solution wherein, this mixture is in Ultrasonic treatment for 2 hours at 40% intensity with 400W sonic horn.

[0053] (2) Collagen cross-linking:

[0054] Add 0.1g collagen to 100mL acetic acid with a concentration of 0.1mol / L, stir at room temperature for 3 hours to obtain a 0.1% (W / V) collagen solution; mix 20mL of the above carbon nanotube dispersion with 100mL collagen solution and 10mL 2mol / L NaOH Mix the solution well; then add 10mL 0.5mol / L 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride...

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Abstract

The invention discloses a conductive collagen heart patch preparation method. The preparation method comprises the steps of preparing carbon nano tube dispersion liquid, crosslinking collagen and preparing a final patch. The method is simple and effective; the prepared conductive collagent heart patch has high mechanical property and strong electron conductivity and further has good biocompatibility, safety and easiness in biodegradation; the limitation that an ordinary heart patching material has poor toughness and electron conductivity and the like in a use process is solved; the conductivecollagen heart patch has a wide application prospect in the field of biological medical materials of tissue engineering and the like.

Description

technical field [0001] The invention relates to the technical field of heart implant materials, in particular to a preparation method of a conductive collagen heart patch. Background technique [0002] Cardiovascular disease (CVD), including myocardial infarction (MI), is associated with high morbidity and mortality worldwide, with 31% of global mortality directly attributable to CVD. Myocardial infarction, the interruption of blood supply to part of the heart leading to cell death and the formation of fibrotic scar tissue, is the leading cause of CVD and is responsible for these deaths. The formation of fibrotic scar tissue on the myocardium leads to weakening of the ventricular wall and causes an increase in diastolic volume caused by collagen slippage and scar thinning and cardiomyocytes located in the myocardium adjacent to the scar affected by new microinfarcts, This process, known as ventricular remodeling, causes dramatic changes in the geometry and shape of the vent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/52A61L27/58
CPCA61L27/443A61L27/52A61L27/58A61L2430/20C08L89/00
Inventor 黄建祥许杉杉孟庆怡
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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