Nitric oxide detection film and preparation method thereof

A technology for nitric oxide and detectors, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve problems such as liquid overflow, inhalation risk, and inconvenience of carrying, and achieve the effects of reaction, high selectivity, and simple film formation

Active Publication Date: 2018-05-01
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with ozone-induced chemiluminescence and gas chromatography-mass spectrometry, which require bulky and expensive instruments, the use of fluorescent probes is a research hotspot. However, small molecule fluorescent probes are all used in liquid phase, and the measured nitric oxide samples It must be compressed and pumped into the liquid, requiring a lot of additional equipment, such as catheters, liquid chambers, pumps, etc., which is inconvenient to carry; and there is a risk of liquid overflow in the liquid chamber, and there is also a risk of inhalation in medical use

Method used

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  • Nitric oxide detection film and preparation method thereof
  • Nitric oxide detection film and preparation method thereof
  • Nitric oxide detection film and preparation method thereof

Examples

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Embodiment 1

[0050] In this example, 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM) was used as the fluorescent probe indicator, and poly(ethylene glycol) diacrylate (PEG-DA) was used as the Polymer dispersant, dimethyl sulfoxide (DMSO) as a dispersion carrier, phosphate buffer solution (PBS) as a buffer, and nylon 6 material as a porous support carrier. The specific preparation is as follows.

[0051] Mix 2 μL of activated 4-amino-5-methylamino-2’,7’-difluorofluorescein, 2 μL of phosphate-buffered saline (PBS, pH 7.4), 2 ml of poly(ethylene glycol) diacrylate , Stir vigorously at room temperature until it is completely dissolved, add 0.5ml of tributyl phosphate and 7ml of dimethyl sulfoxide to it to make an indicator formula solution. Place a square hydrophobic porous membrane matrix material with a side length of 2cm horizontally on a square glass plate with a side length of 5cm, then add 2ml of the indicator formula solution to the center of the porous membrane material dropw...

Embodiment 2

[0053] In this example, 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM) was used as the fluorescent probe indicator, and polyhydroxyethyl methacrylate (Poly-HEMA) was used as the polymer As a dispersant, toluene is used as a dispersion carrier, and polyethylene terephthalate (PET) is used as a porous support carrier. The specific preparation is as follows.

[0054] Mix 7.5 μg of activated 4-amino-5-methylamino-2',7'-difluorofluorescein, 2 mg of polyhydroxyethyl methacrylate, add 0.5 ml of tributyl phosphate and 7 ml of Toluene, made into indicator formula solution. Place a square polyethylene terephthalate porous membrane matrix material with a side length of 2cm horizontally on a square glass plate with a side length of 5cm, then add 2ml of the indicator formula solution dropwise to the center of the porous membrane material, and wait for the solution to automatically After the membrane material is covered, soak for 90 seconds, blot the excess solution, and dry nat...

Embodiment 3

[0056] In this example, 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM) was used as the fluorescent probe indicator, ethyl cellulose was used as the polymer dispersant, and toluene As a dispersion carrier, polyethylene terephthalate (PET) was used as a porous support carrier. The specific preparation is as follows.

[0057]Mix 7.5 μg of activated 4-amino-5-methylamino-2',7'-difluorofluorescein and 2 mg of ethyl cellulose, add 0.5 ml of tributyl phosphate and 7 ml of toluene, and prepare into an indicator formula solution. Place a square polyethylene terephthalate porous membrane matrix material with a side length of 2cm horizontally on a square glass plate with a side length of 5cm, then add 2ml of the indicator formula solution dropwise to the center of the porous membrane material, and wait for the solution to automatically After the membrane material is covered, soak for 90 seconds, blot the excess solution, and dry naturally for 2 hours to obtain the nitric oxid...

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Abstract

The invention provides a nitric oxide detection film, which is characterized by comprising a porous thin film, and a fluorescence probe indicator and a polymeric dispersant attached to the porous thinfilm.

Description

technical field [0001] The invention generally relates to the field of nitric oxide detection, in particular to a nitric oxide fluorescence detection film and a preparation method thereof. Background technique [0002] Nitric oxide (NO) is an important air pollutant, which is mainly produced in the process of fuel combustion, and automobile exhaust and boiler flue gas are the most important sources of NO pollution. Nitric oxide emitted into the air is mostly oxidized into nitrogen dioxide, which is an important cause of acid rain. Nitrogen dioxide can combine with ozone and hydrocarbons in the atmosphere to produce photochemical smog, which can cause major environmental events such as photochemical smog. Therefore, it is very important to detect the content of nitric oxide in pollution sources. From an environmental point of view, nitric oxide is also a source of air pollution, and it is commonly found in industrial smoke, automobile exhaust, and decoration paint. Nitric o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6439
Inventor 洪梅张健陈柱文陈超王彦顶白杉董磊
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL
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