Method for extracting levodopa from mucuna pruriens by continuous countercurrent ultrasonic technique

Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a method for extracting levodopa from mucuna pruriens by a continuous countercurrent ultrasonic technique. The method comprises carrying out extraction on mucuna pruriens medicinal materials by adopting the continuous countercurrent ultrasonic method, carrying out filtering by an acid membrane, and carrying out recrystallization to obtain a levodopa crystal. Through detection, the purity of the levodopa product obtained by adopting the method is 99% or above, and the yield is 5.0% or above. And meanwhile, the protein in the mucuna pruriens dregs can be extracted by using alkali liquor. According to the method provided by the invention, levodopa can be rapidly and efficientlyextracted from the mucuna pruriens in a large scale, and after the filtering, the filtrate directly flows back and is used as an extraction liquid for recycling. The product has a short production period, low energy consumption and less environmental pollution.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and relates to a method for extracting and preparing medicinal materials, in particular to a method for extracting levodopa from cat beans by using continuous countercurrent ultrasonic extraction equipment. Background technique [0002] Cat bean is a traditional Chinese herbal medicine plant resource in my country. Utilis) are mainly distributed in Guangxi, Guizhou and other places in my country. Bean shells, bean leaves, bean vines and bean seeds are rich in crude fat and crude protein, starch, various vitamins and other nutrients. They can be used as livestock feed and excellent additives in the food industry. In terms of medicinal value, cat bean is one of the important raw materials for extracting levodopa. [0003] Levodopa (English name Levodopa, referred to as L-DOPA) is an important biologically active substance in the body, an important intermediate product in the biochemical metabol...

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Problems solved by technology

[0008] ①The announcement number is CN102267919A. The method uses cat beans as raw material and acid aqueous solution as extraction solvent. After extraction, water-soluble organic solvent, salt solution and polymer water-soluble polymer form a two-phase system in proportion. The average concentration in the solution reaches 20-40% distribution balance, then extracting with an extractant immiscible with the two water phases, removing impurities respectively, and the remaining part is 99.9% levodopa product with a mass fraction of 99.9% through decolorization, crystallization and drying; in this method A huge amount of ethanol is required, which brings inconvenience to subsequent treatment, purification, recycling and reuse

[0010] ③ The patent with the publication number CN103467328 proposes subcritical extraction under acidic conditions. After the cat beans are crushed, subcritical extraction is carried out with hydrochloric acid with a mass fraction of 1‰~3‰. After the extract is concentrated, it is left to stand overnight and crystallized , to obtain the crude extract; after the crude extract is dissolved with dilute hydrochloric acid, add activated carbon to reflux, then filter to remove the activated carbon, adjust the pH of the filtrate to 3.0-4.0 with an alkaline agent, leave it to stand overnight, crystallize, and dry to obtain the finished product; the final product The yield is 2.8%~3.0%, and the content can reach 98%. Although the extraction process does not add a large amount of inorganic salts and other substances, avoiding excessive heavy metals, etc., the product yield is low and the purity is not high.

Moreover, the extraction time is relatively long, and the high pressure is maintained for a long time, and the non-continuous production is not conducive to improving the industrial production cycle.

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